Genetic control of arbuscular mycorrhizal colonization by Rhizophagus intraradices in Helianthus annuus (L.)

Abstract

Supporting InformationData S1. Root colonization data used for genome-wide association mapping. All metrics reflect percent of root length colonized with arbuscular mycorrhizal fungi. Mean Colonization Rate (LS Mean) is the least squares mean of root colonization for each line (genotype) when corrected for spatial gradients in the greenhouse. Maximum Colonization is the highest colonization rate of the four biological replicates of each line. Range of Colonization represents the difference in colonization rate between the biological replicate with the highest colonization rate and the lowest colonization rate for each line. The Inclusion in GWAS column indicates the lines that were included in genome-wide association analysis, after 27 lines were excluded due to high residual heterozygosity.

Candidate pathway association and genome‐wide association approaches reveal alternative genetic architectures of carotenoid content in cultivated sunflower (Helianthus annuus)

“…(Ueno et al, 2014). However, neither the transcriptome changes induced by arbuscular mycorrhizal fungi nor the results of an LMM GWAS of mycorrhizal associations in cultivated sunflower implicated β‐carotene isomerase or any of the other putative carotenoid‐influencing genes identified in the present study (Vangelisti et al, 2018; Stahlhut et al, 2021). While products of the carotenoid pathway can influence mycorrhizal relationships, other genetic markers, such as potassium ion channels and plant immunity gene variation, are the primary correlates of these associations in sunflower (Vangelisti et al, 2018; Stahlhut et al, 2021).…”

“…However, neither the transcriptome changes induced by arbuscular mycorrhizal fungi nor the results of an LMM GWAS of mycorrhizal associations in cultivated sunflower implicated β‐carotene isomerase or any of the other putative carotenoid‐influencing genes identified in the present study (Vangelisti et al, 2018; Stahlhut et al, 2021). While products of the carotenoid pathway can influence mycorrhizal relationships, other genetic markers, such as potassium ion channels and plant immunity gene variation, are the primary correlates of these associations in sunflower (Vangelisti et al, 2018; Stahlhut et al, 2021). Further integrating our findings, if the presence or absence of carlactones has a minimal impact on mycorrhizal associations in cultivated sunflower, future breeding work to combat parasitic plants may target β‐carotene isomerase to either reduce its enzyme efficiency or reduce its gene expression.…”

Broad diversity in monoterpene–sesquiterpene balance across wild sunflowers: Implications of leaf and floral volatiles for biotic interactions

“…Together, these may be a more fruitful avenue to leveraging crop wild relatives for the improvement of target biotic interactions, permitting a focus on increasing or decreasing the abundance of individual compounds while working wholly within the H. annuus genetic background. Identifying the genetic architecture of variation in the expression of individual terpene compounds within cultivated sunflower is achievable with currently available association‐mapping resources for sunflower (Masalia et al, 2018; Stahlhut et al, 2021; Todesco et al, 2022) and thus may permit more straightforward up‐ or downregulation through either traditional breeding or emerging gene editing methods (Wang et al, 2020; Pan et al, 2022).…”

Exceeding expectations: the genomic basis of nitrogen utilization efficiency and integrated trait plasticity as avenues to improve nutrient stress tolerance in cultivated sunflower (Helianthus annuusL.)

“…The SAM population includes both heterotic groups (i.e., male [RHA] and female [HA] lines) as well as both major market types (i.e., oil and non-oil [confectionery] lines). The SAM population has been used extensively for GWAS studies (Masalia et al ., 2018; Gao et al ., 2019; Temme et al ., 2020; Stahlhut et al ., 2021) because of the substantial genetic/trait diversity contained within the population, relevant commercial uses, and the availability of whole genome re-sequencing data for the entire population.…”