Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus

Lee, Nam-Hyung; Song, Hyun-Jung; Park, Chang-Soo; Kim, Hee-Dai; Park, Kwon-Sam

doi:10.5657/kfas.2011.0597

Cited by 2 publications

(2 citation statements)

References 20 publications

(7 reference statements)

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“…PCR assay, real-time PCR assay LAMP assay (Kim et al, 1999;Kim et al, 2008;Chen and Ge, 2010;Yu et al, 2010;Hossain et al, 2011;No et al, 2011;Liu et al, 2012;Wang et al, 2013). RIMD2210633 -lactamase (VPA0477) 283 31.7 kDa (Makino et al, 2003 (Lee et al, 2011). , -lactamase (VPA0477)…”

Section: 서 론mentioning

confidence: 99%

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Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction

Park¹

2014

Korean Journal of Fisheries and Aquatic Sciences

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In this study, the β-lactamase (VPA0477) gene was used as a new target for the PCR-based detection of Vibrio parahaemolyticus. Primers specific for the β-lactamase (VPA0477) gene of V. parahaemolyticus, were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and non-Vibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The β-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

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Section: 서 론mentioning

confidence: 99%

“…PCR assay 108 67 -lactamase (VPA0477) (Lee et al, 2011;Kim et al, 2014), Pazhani et al (2014) 178 -lactamase (VPA0477) .…”

Section: 서 론unclassified

Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction

Park¹

2014

Korean Journal of Fisheries and Aquatic Sciences

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Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area

김태옥¹,

엄인선²,

조상만³

et al. 2014

Korean Journal of Fisheries and Aquatic Sciences

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Sixty-seven Vibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacillin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibitory concentration (MIC) of the 67 V. parahaemolyticus isolates to ampicillin and oxacillin ranged from 512−2,048 and 64−512 µg/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh. The VPA0477 (β-lactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V. parahaemolyticus.

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Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus

Cited by 2 publications

References 20 publications

Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction

Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction

Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area

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