“…However, genotyping with a single marker does not allow identification of nonclonal strains, and to determine more precisely the presence of polymorphisms in the population, application of multilocus PCR-RFLP and microsatellite analysis of multiple markers is necessary (Ajzenberg et al, 2005;Su et al, 2006). Although in our experience the GRA6 gene was, due to a small amount of T. gondii DNA, the only amplified marker in a blood sample of a neonate suspected of congenital toxoplasmosis (Table 3), that clearly indicated the presence of type I, in our laboratory genotyping is regularly performed using SAG1, SAG2, GRA6 and GRA7 as markers (Miller et al, 2004;Dubey et al, 2007;Prestrud et al, 2008;Richomme et al, 2009;Aubert et al, 2010).…”