Genetic characterization of subtype H1 avian influenza viruses isolated from live poultry markets in Zhejiang Province, China, in 2011

Abstract

Nine avian influenza A viruses (AIVs), H1N2 (n = 2) and H1N3 (n = 7), were isolated from domestic ducks in live poultry markets in Zhejiang Province, Eastern China, in 2011. All viruses were characterized by whole genome sequencing with subsequent phylogenetic analysis and genetic comparison. Phylogenetic analysis of all eight viral genes showed that the viruses clustered in the Eurasian lineage of influenza A viruses. The hemagglutinin cleavage site of all viruses displayed features of a monobasic cleavage site. Although there was no evidence of re-assortment in subtype H1 AIVs among the avian species and mammalian hosts in this study, continued surveillance is needed considering the important role of the domestic duck in the dissemination and re-assortment of AIVs.

Complete Genomic Sequence of a Novel Reassortant H11N3 Influenza Virus Isolated from Domestic Ducks in Jiangsu, China

“…The remaining seven genes all had the greatest sequence identities (over 99.0%) with the lowpathogenic H1N3 and H7N3 viruses isolated from domestic ducks in Zhejiang Province in 2011 (10,12). In addition, the PB2 and NP genes had highly similar nucleotide sequence identities of 96.3% and 96.1%, respectively, to the previously reported reassortant pathogenic virus A/duck/Zhejiang/2245/2011(H5N1) (11).…”

Characterization of Novel Reassortant Influenza A (H5N2) Viruses Isolated from Poultry in Eastern China, 2015

“…Each swab was eluted with 2.0 mL phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA), 4 × 10 6 U/L penicillin G, and 400 mg/L streptomycin sulfate. A 0.22-μm filter was used to decontaminate the samples, which were then inoculated into the allantoic cavities of 9-day-old specific pathogen-free embryonated eggs as described previously (Wu et al, 2012). After incubation at 37°C for 72 h, the allantoic fluid was harvested and viral titers were determined by hemagglutination (HA) assay using a standard method (Klimov et al, 2012).…”

Novel reassortant H10N7 avian influenza viruses isolated from chickens in Eastern China

“…The viruses were isolated from each sample and was inoculationed into embryonated chicken eggs as described elsewhere [7]. RNA was extracted using the viral RNA mini kit (Qiagen), according to the manufacturer's instructions.…”