Genetic characterization of some Turkish sheep breeds based on the sequencing of the <i>Ovar-DRB1</i> gene in the major histocompatibility complex (MHC) gene region

Abstract

In this research, Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region was surveyed by DNA sequencing in some of the native sheep breeds that are reared in Turkey. A total of 80 samples were collected from eight different Turkish native sheep breeds, and these samples were used for DNA sequencing.The exon 2 region of Ovar-DRB1 in the MHC gene region was polymerase chain reaction (PCR) amplified and sequenced. A total of 25 new alleles were revealed in the Ovar-DRB1 gene in Turkish native sheep breeds with 24 variable sites; only 13 sites were parsimony informative. The average pairwise genetic distance was 0.029 % for the Ovar-DRB1 gene exon 2 region. The sequence variations at eight different positions (7026, 7036, 7040, 7053, 7059, 7069, 7131 and 7214) are found in all of the studied samples. G → C transversion at position 7081 is only seen in Akkaraman sheep breed, whereas T → C transition at position 7097 is only seen in one sample from the Akkaraman breed. Overall, two main groups were detected among the 25 alleles from Turkish native sheep breeds. All Daǧliç and Kivircik alleles and one allele from Karayaka, Malya and Sakiz are grouped together while all the other breeds are grouped in the other branch.

An overview of remote monitoring methods in biodiversity conservation

“…Different restriction sites in DNA represent the genetic divergence between different populations or related species within a population. Özdil et al ( 2018 ) demonstrated genetic diversity among 11 donkey species in Turkey by conducting PCR-RFLP of two genes. The restriction sites of DraII, MboI, and EagI on the lactoferrin gene (LTF) and PstI on the κ-casein gene (CSN3) have been validated to identify the polymorphism among the donkey population (Özdil et al 2018 ).…”

“…Özdil et al ( 2018 ) demonstrated genetic diversity among 11 donkey species in Turkey by conducting PCR-RFLP of two genes. The restriction sites of DraII, MboI, and EagI on the lactoferrin gene (LTF) and PstI on the κ-casein gene (CSN3) have been validated to identify the polymorphism among the donkey population (Özdil et al 2018 ). Meikasari et al ( 2019 ) have also made elucidation of low genetic diversity among the seahorse ( Hippocampus comes ) found in Bintan waters.…”

“…2 Molecular techniques for conservation of biodiversity. A Restriction fragment length polymorphism; the genomic DNA extracted from different organisms is PCR amplified and subjected to restriction digestion using specific restriction enzymes, then DNA fragments are separated by electrophoresis and hybridized with radiolabeled probes (Özdil et al 2018 ; Chaudhary and Maurya 2019 ; Panigrahi et al 2019 ; Haider and Nabulsi 2020 ). B Amplified fragment length polymorphism; the restriction fragments of genomic DNA was ligated with compatible adapters and PCR amplified using selective primers against adapters, and the amplified fragments were separated by electrophoresis for DNA fingerprint analysis (Blears et al 1998 ; Malik et al 2018 ; Wu et al 2019b ; Zimmermann et al 2019 ; Neiber et al 2020 ).…”

Exploring genetic diversity and variation of Ovar-DRB1 gene in Sudan Desert Sheep using targeted next-generation sequencing

“…In recent studies, the genetic diversity of native sheep breeds reared in Turkey and Algeria has been investigated [ 6 , 7 ]. These studies aimed to explore the impact of polymorphisms in the MHC gene region, which has the potential to influence future animal breeding strategies.…”