Genetic Characterization of <i>Toxoplasma gondii</i> Isolates from Pigs in Jilin Province, Northeastern China

Jiang, Haihai; Wang, Shuchao; Huang, Si-Yang; Zhao, Lei; Wang, Zedong; Zhu, Xing‐Quan; Liu, Quan

doi:10.1089/fpd.2015.2043

Cited by 18 publications

(6 citation statements)

References 33 publications

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“…The prevalence of T. gondii differs greatly among countries and regions within the same country. In the present study, the prevalence of T. gondii was 2.06% in pigs, a value lower than those reported in other studies in China [11–13]. Because of the lack of existing epidemiological data on swine toxoplasmosis in the areas we investigated, we cannot speculate as to why a low toxoplasma prevalence rate was obtained.…”

Section: Discussioncontrasting

confidence: 86%

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

et al. 2017

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Background Toxoplasma gondii, a widely prevalent protozoan parasite, causes serious toxoplasmosis infections in humans and other animals. Among livestock, pigs are susceptible to T. gondii infection. Despite Henan being one of the biggest pig-raising provinces in China, little information exists on the epidemiology of toxoplasmosis in this location. Therefore, we molecularly characterized DNA samples from pigs in Henan. A total of 1647 samples, including 952 from dead piglets, 478 from seriously sick fattening pigs and 217 from abortion sows, were collected from different animal hospitals or pig farms from 10 different cities in Henan (2006–2008). Each pig corresponded to a separate pig farm. DNA was extracted from 3 to 5 g of the most severely affected pig tissue (liver, spleen, lung, hilar lymph nodes and amniotic fluid) after postmortem examination. The presence of the T. gondii B1 gene was detected using nested polymerase chain reactions (PCR). Genotyping was performed directly on DNA from the PCR-positive tissue samples using 11 PCR restriction fragment length polymorphism markers (SAG1, 5′- and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22–8, c29–2, and Apico).ResultsOf all samples, thirty-four were positive for the T. gondii B1 gene (2.06%, 95% CI: 1.86%–2.26%) from four cities, including 31 from NanYang city, one (PgXY 1) from Xinyang City, one (PgZZ 1) from Zhengzhou City and one (PgZK1) from Zhoukou City. The prevalence was found to be highest in piglets than in fattening pigs and sows. And the difference was statistically significant (P<0.01). The following 32 samples were genotyped with complete data: 13 hilar lymph node tissue samples, seven liver tissue samples, seven lung tissue samples, four spleen tissue samples, and one amniotic fluid sample. Only one genotype, belonging to ToxoDB Genotype #9, was identified.ConclusionsThis is the first large-scale survey molecularly characterizing T. gondii from pigs in Henan. The results of the present study revealed that T. gondii infection is present in swine in Henan and is a potential source of foodborne toxoplasmosis in the investigated areas. Implementation of effective control measures for T. gondii to reduce the chance of zoonotic toxoplasmosis spreading from pig farms may be warranted. The results show that the ToxoDB #9 genotype may be the dominant T. gondii lineage in mainland China. These findings strengthen the limited Chinese T. gondii epidemiology database.

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Section: Discussioncontrasting

confidence: 86%

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

et al. 2017

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“…Two T. gondii strains were isolated from chickens and were genotyped as ToxoDB#225 and Type I ( Zhao et al, 2012 ; Wang et al, 2015 ). Forty-five T. gondii strains were isolated from swines and genotyped as ToxoDB#9, ToxoDB#3, and ToxoDB#1 ( Zhou et al, 2009 ; Jiang et al, 2013 , 2015 ; Li et al, 2015 ; Wang et al, 2015 , 2016 ). Seven T. gondii strains were isolated from humans and genotyped as ToxoDB#1, ToxoDB#9, ToxoDB#3, ToxoDB#10, ToxoDB#204, and ToxoDB#4 ( Fu et al, 2015 ).…”

Section: The Molecular Epidemiology Of T Gondii Imentioning

confidence: 99%

Prevalence, Risk Factors, and Genotypes of Toxoplasma gondii in Food Animals and Humans (2000–2017) From China

Dong

et al. 2018

Front. Microbiol.

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Toxoplasma gondii as a food-borne pathogen, the infection of it in food animals has relation with human toxoplasmosis, but the trends and epidemiological features of T. gondii infections in food animals are rarely studied in China. The aimed of this study was to assess the epidemiology and risks of T. gondii in sheep, goats, swines, chickens, yaks, cattle and humans from 2000 to 2017 and to explore prevention and control strategies. The overall seroprevalence of T. gondii infections in food animals is 23.7% (39,194/165,417, 95%CI, 23.49–23.90%), which is significantly higher than that in humans (8.2%, 95%CI, 8.06–8.39%, 8,502/103,383) (P < 0.0001). Compared the prevalence of T. gondii infections in animals and humans sampled from 2000 to 2010, it was significantly increased in the period 2011 to 2017 (P < 0.0001). Compared the food animals from non-Yangtze River, animals from regions of the Yangtze River have high seroprevalence rates for T. gondii (P < 0.0001). Furthermore, samples from the western to eastern regions of the Yellow River showed an increase in seroprevalence for T. gondii (P < 0.0001). It was speculated that T. gondii oocysts may be transmitted by water and annual precipitation possible help the oocyst spread and retain accessible for potential hosts. Effective prevention and control strategies are including water filtration or water boiling, inactivating oocysts from feline’s feces, monitoring birds and rodents. Chinese 1 (ToxoDB#9) is the predominant genotype in food animals from China.

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“…The T. gondii genotypes prevailing in China are ToxoDB #1, ToxoDB #2, ToxoDB #3, ToxoDB #9, ToxoDB #10, ToxoDB #20, ToxoDB #204, ToxoDB #205 and ToxoDB #225 (7,18–20). ToxoDB #9 was identified in pigs in Sichuan Province (14).…”

Section: Discussionmentioning

confidence: 99%

“…Multilocus PCR-RFLP method targeting the 11 genetic markers (i.e., SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) was used to genetically characterize the positive DNA samples as described previously (10, 12–14).…”

Section: Methodsmentioning

confidence: 99%

Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

Wang

Dong

Zhang

et al. 2019

IJPA

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Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

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Genetic Characterization of Toxoplasma gondii Isolates from Pigs in Jilin Province, Northeastern China

Cited by 18 publications

References 33 publications

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

Prevalence, Risk Factors, and Genotypes of Toxoplasma gondii in Food Animals and Humans (2000–2017) From China

Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

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