Genetic characterization of an X-STR decaplex on the population of Pichincha (Ecuador)

Gaviria, A.; Zambrano, Ana Karina; Morejon, G.; Aguirre, V.; Vela, M.; Builes, J.J.; Burgos, Germán

doi:10.1016/j.fsigss.2013.10.075

Cited by 5 publications

(8 citation statements)

References 4 publications

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“…The maximum frequency was of 0.4867 for allele 10 (DXS8378). The frequencies for loci DXS7132, DXS8378, DXS7423 are comparable to the study carried out in the province of Pichincha -Ecuador [6] where DXS8378 has the highest frequency reported of 0.6038 (allele 10). For the Ecuadorian population, the most polymorphic marker was the DXS10135 locus, with the highest heterozygosity observed (Ho = 0.9300) and power of exclusion (PE = 0.8570), polymorphism information content (PIC F = 0.9313), T Table 1 Allele frequencies for twelve X-STR markers of the Ecuadorian population.…”

Section: Resultssupporting

confidence: 82%

“…In addition, due to their high polymorphism information content, they are very informative for population studies of diverse ethnic groups [3]. The present study analyzes different forensic parameters in 12 X-STRs of which there have 8 new markers (DXS10148, DXS10135, DXS10079, DXS10074, DXS10103, DXS10101, DXS10146 and DXS10134) compared to decaplex multiplex system previously used [6].…”

Section: Introductionmentioning

confidence: 99%

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Genetic polymorphisms of 12 X-STRs in the Ecuadorian population

Gaviria¹,

Boada²,

Rodríguez-Pólit³

et al. 2019

Forensic Science International: Genetics Supplement Series

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The X-STR markers represent a powerful tool to complement the information provided by the autosomal and Y chromosome markers, particularly, in cases involving at least one woman. In the present study, allele frequencies, power of exclusion (PE), power of discrimination (PD) and polymorphism information content (PIC) were analyzed for 12 X chromosome loci. A total of 200 unrelated Ecuadorian individuals were selected for the population analysis. The data showed that DXS10135 locus had the highest power of discrimination value (PD = 0.9351), and polymorphism information content (PIC = 0.9313). The heterozygosity observed ranged from 0.9300 to 0.6400. The power of exclusion varied between 0.3417 to 0.8570 for DXS10135 and DXS8378 respectively. No significant departure from Hardy Weinberg equilibrium (HWE) was detected for any of the analyzed markers. The X-STR markers have demonstrated their potential to efficiently complement the analysis to solve complex kinship and forensic cases.

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms of 12 X-STRs in the Ecuadorian population

Gaviria¹,

Boada²,

Rodríguez-Pólit³

et al. 2019

Forensic Science International: Genetics Supplement Series

View full text Add to dashboard Cite

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“…All the samples had different X-STR profiles and the subjects were not closely related through the maternal line. We compared these data to the ethnic groups from Ecuador [ 20 , 26 , 27 ]. None of the resulting profiles had been previously observed in these populations.…”

Section: Resultsmentioning

confidence: 99%

The genetic evidence for human origin of Jivaroan shrunken heads in collections from the Polish museums

et al. 2016

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Advances in forensic identification using molecular genetics are helpful in resolving some historical mysteries. The aim of this study was to confirm the authenticity of shrunken-head artifacts exhibited by two Polish museums. Shrunken heads, known as tsantsas, were headhunting trophies of South American Indians (Jivaroan). A special preparation preserved their hair and facial appearance. However, it was quite common to offer counterfeit shrunken heads of sloths or monkeys to collectors of curiosities. We sampled small skin specimens of four shrunken-head skin from the museum collection from Warsaw and Krakow, Poland. Following genomic DNA isolation, highly polymorphic short tandem repeats were genotyped using a commercial chemistry and DNA sequencing analyzer. Haplogroups of human Y chromosome were identified. We obtained an informative genetic profile of genomic short tandem repeats from all the samples of shrunken heads. Moreover, amplification of amelogenin loci allowed for sex determination. All four studied shrunken heads were of human origin. In two ones, a shared Y-chromosome haplogroup Q characteristic for Indigenous Americans was detected. Another artifact was counterfeited because Y-chromosome haplogroup I2 was found, characteristic for the Southeastern European origin. Commercial genetic methods of identification can be applied successfully in studies on the origin and authenticity of some unusual collection items.

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“…DNA was extracted using FTA buffer reagent according to manufacturer's recommendations (Whatman-GE Healthcare). X-STR amplification was performed in accordance with Gusmão et al [6], including some modifications by Gaviria et al [7]. Alleles were separated and detected using the 3500 Genetic Analyzer (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…Allele frequencies, Heterozygosity, power discrimination (PD), and Polymorphism Information Content (PIC) were calculated with FORSTAT v1.0 [8]; Hardy-Weinberg equilibrium and linkage disequilibrium were tested with GenePop v4.2 [9] and power of exclusion (PE) in trios and duos were calculated according Desmarais et al in Microsoft Excel [10]. Using the platform GenAlEx [11], pairwise population comparison for the 10 X-STRs (F ST genetic distance analysis), and an Analysis of Molecular Variance (AMOVA) were performance between Imbabura, Pichincha province and Ecuador [7,12].…”

Section: Methodsmentioning

confidence: 99%

X-STR decaplex study in the population of Imbabura-Ecuador

Zambrano

Vaca-Pólit²,

Boada³

et al. 2019

Forensic Science International: Genetics Supplement Series

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X chromosome STR markers are a useful tool in population genetics, they provide supplementary information for forensic and kinship analysis where autosomal and Y chromosome information is not enough. A total of 100 unrelated individuals from Imbabura-Ecuador were genotyped using the X-STR Decaplex system (DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423). Allele frequencies and population parameters were calculated for each STR. The most informative marker was DXS6809 and the least informative was DXS7133. High discrimination power values were obtained (greater than 99.99%), as well as high mean exclusion chance in trios (mother / daughter / father) 0.9999948011 and duos (father/daughter) 0.9992880836. The genetic distance (F ST ) obtained comparing the Ecuadorian population and the Pichincha province population show low genetic distances. The data analyzed confirmed the potential of these 10 X-STRs.

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Genetic characterization of an X-STR decaplex on the population of Pichincha (Ecuador)

Cited by 5 publications

References 4 publications

Genetic polymorphisms of 12 X-STRs in the Ecuadorian population

Genetic polymorphisms of 12 X-STRs in the Ecuadorian population

The genetic evidence for human origin of Jivaroan shrunken heads in collections from the Polish museums

X-STR decaplex study in the population of Imbabura-Ecuador

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