Genetic characterization of a novel recombinant PRRSV2 from lineage 8, 1 and 3 in China with significant variation in replication efficiency and cytopathic effects

Han, Guangwei; Lei, Kaixia; Xu, Huiling; He, Fang

doi:10.1111/tbed.13491

Cited by 16 publications

(16 citation statements)

References 54 publications

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“…It is suspected that imported breeding pigs from the US were the source of the virus introduction. It was also shown later that NAD30-like PRRSVs underwent recombination with various co-circulating PRRSV2 strains in China including the classical Chinese PRRSV2, the previous dominant HP-PRRSVs [15,64,65] and even the MLV-related strains such as RespPRRS MLV [66] and the Chinese HP-PRRSV JXA1-R based MLV [16].…”

Section: Asfvmentioning

confidence: 95%

Major swine viral diseases: an Asian perspective after the African swine fever introduction

2020

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Asia is a major pig producer of the world, and at present, African swine fever virus (ASFV) continues to significantly impact the Asian pig industry. Since more than 50% of the world's pig population is in Asia, ASFV outbreaks in Asia will affect the global pig industry. Prior to the introduction of ASF, several outbreaks of major swine viruses occurred in Asia over the last two decades, including porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and foot and mouth disease virus (FMDV). The rapid spreading of those viruses throughout Asia involve many factors such as the various pig production systems and supply chains ranging from backyard to intensive industrial farms, animal movement and animal product trading within and among countries, and consumer behaviors. ASF has notoriously been known as a human-driven disease. Travelers and international trading are the major ASFV-carriers for the transboundary transmission and introduction to naïve countries. Globalization puts the entire pig industry at risk for ASF and other infectious diseases arising from Asian countries. Disease control strategies for the various pig production systems in Asia are challenging. In order to ensure future food security in the region and to prevent the deleterious consequences of ASF and other major viral disease outbreaks, disease control strategies and production systems must be improved and modernized.

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Section: Asfvmentioning

confidence: 95%

Major swine viral diseases: an Asian perspective after the African swine fever introduction

2020

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Section: Discussionmentioning

confidence: 99%

“…The current commercial vaccines show limited protection in infected piglets. The extensive recombination phenomenon among NADC30-like PRRSVs is a distinctive characteristic of PRRSV ( Wang et al, 2018 ; Zhou et al, 2018 ; Han et al, 2020 ). In dealing with complex epidemic situations, there is an urgent need to establish a good challenge model to evaluate the current vaccine candidates in preventing infection with a heterologous strain.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Bivalent Live Vectored Vaccine Against Classical Swine Fever and HP-PRRS Revealed Adequate Heterogeneous Protection Against NADC30-Like Strain

Chen

Cao

et al. 2022

Front. Microbiol.

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The recombinant bivalent live vectored vaccine rPRRSV-E2 has been proved to be a favorable genetic engineering vaccine against classical swine fever (CSF) and highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS). NADC30-like strains have recently emerged in China and caused severe disease, and it is necessary to evaluate the vaccine candidate for the currently circulating viruses. This study established a good challenge model to evaluate the candidate rPRRSV-E2 vaccine in preventing infection with a representative NADC30-like strain (ZJqz21). It was shown that the challenge control piglets displayed clinical signs typical of PRRSV, including a persistent fever, dyspnea, moderate interstitial pneumonia, lymph node congestion, and viremia. In contrast, the rPRRSV-E2 vaccination significantly alleviated the clinical signs, yielded a high level of antibodies, provided adequate protection against challenge with ZJqz21, and inhibited viral shedding and the viral load in target tissues. Our results demonstrated that the recombinant bivalent live vectored vaccine strain rPRRSV-E2 can provide efficient protection against the challenge of heterologous circulating NADC30-like strain and could be a promising vaccine candidate for the swine industry.

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“…Additionally, the Japanese strain Jpn5-37 induces moderate symptoms in animals [ 31 ]. Some NADC30-like strains in China show high pathogenicity (JL580, SD17-38, 14LY01-FJ, 14LY02-FJ, 15LY01-FJ, 15LY02-FJ, FJXS15, HBap4-2018, JS18-3) [ 25 , 32 , 33 , 34 , 35 ]; however, most strains show moderate pathogenicity (HNjz15, CHsx1401, SD53-1603, SC-d, TJnh1501, SCN17, HB17A, SCya18, HN201605, FJZ03, FJWQ16, ZJqz21, v2016/ZJ/09-03, FJ1402) [ 26 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Based on cumulative data, recombination may be responsible for the pathogenicity variance of NADC30-like PRRSVs in China, and the pathogenicity tends to be intermediate between those of the parental strains [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets

Zhang

Xiang

et al. 2022

Vaccines

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Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that endangers the swine industry worldwide. Recently, lineage 1 PRRSVs, especially NADC30-like PRRSVs, have become the major endemic strains in many pig-breeding countries. Since 2016, NADC30-like PRRSV has become the predominant strain in China. Unfortunately, current commercial vaccines cannot provide sufficient protection against this strain. Here, an attenuated lineage 1 PRRSV strain, named SD-R, was obtained by passaging an NADC30-like PRRSV strain SD in Marc-145 cells for 125 passages. Four-week-old PRRSV-free piglets were vaccinated intramuscularly with 105.0TCID50 SD-R and then challenged intramuscularly (2 mL) and intranasally (2 mL) with homologous NADC30-like PRRSV SD (1 × 105.0TCID50/mL) and heterologous NADC30-like PRRSV HLJWK108-1711 (1 × 105.0TCID50/mL). The results showed that antibodies against specific PRRSVs in 5 of 5 immunized piglets were positive after a 14-day post-vaccination and did not develop fever or clinical diseases after NADC30-like PRRSV challenges. Additionally, compared with challenge control piglets, vaccinated piglets gained significantly more weight and showed much milder pathological lesions. Furthermore, the viral replication levels of the immunized group were significantly lower than those of the challenge control group. These results demonstrate that lineage 1 PRRSV SD-R is a good candidate for an efficacious vaccine, providing complete clinical protection for piglets against NADC30-like PRRSVs.

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Genetic characterization of a novel recombinant PRRSV2 from lineage 8, 1 and 3 in China with significant variation in replication efficiency and cytopathic effects

Cited by 16 publications

References 54 publications

Major swine viral diseases: an Asian perspective after the African swine fever introduction

Major swine viral diseases: an Asian perspective after the African swine fever introduction

Recombinant Bivalent Live Vectored Vaccine Against Classical Swine Fever and HP-PRRS Revealed Adequate Heterogeneous Protection Against NADC30-Like Strain

Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets

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