Genetic Basis of Natural Variation in Spontaneous Grooming in<i>Drosophila melanogaster</i>

Yanagawa, Aya; Huang, Wen; Yamamoto, Akihiko; Wada‐Katsumata, Ayako; Schal, Coby; Mackay, Trudy F. C.

doi:10.1534/g3.120.401360

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Genetic factors have been implicated in spontaneous (i.e., unstimulated) grooming behavior in Drosophila melanogaster ( Yanagawa et al, 2020 ) and in other drosophilid species ( Hernández et al, 2020 ). Our results demonstrate that this is true for dust-induced grooming as well.…”

Section: Discussionmentioning

confidence: 99%

Variation and Variability in Drosophila Grooming Behavior

Mueller

Zhang

Carlson

et al. 2022

Front. Behav. Neurosci.

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Behavioral differences can be observed between species or populations (variation) or between individuals in a genetically similar population (variability). Here, we investigate genetic differences as a possible source of variation and variability in Drosophila grooming. Grooming confers survival and social benefits. Grooming features of five Drosophila species exposed to a dust irritant were analyzed. Aspects of grooming behavior, such as anterior to posterior progression, were conserved between and within species. However, significant differences in activity levels, proportion of time spent in different cleaning movements, and grooming syntax were identified between species. All species tested showed individual variability in the order and duration of action sequences. Genetic diversity was not found to correlate with grooming variability within a species: melanogaster flies bred to increase or decrease genetic heterogeneity exhibited similar variability in grooming syntax. Individual flies observed on consecutive days also showed grooming sequence variability. Standardization of sensory input using optogenetics reduced but did not eliminate this variability. In aggregate, these data suggest that sequence variability may be a conserved feature of grooming behavior itself. These results also demonstrate that large genetic differences result in distinguishable grooming phenotypes (variation), but that genetic heterogeneity within a population does not necessarily correspond to an increase in the range of grooming behavior (variability).

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Section: Discussionmentioning

confidence: 99%

Variation and Variability in Drosophila Grooming Behavior

Mueller

Zhang

Carlson

et al. 2022

Front. Behav. Neurosci.

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“…One limitation of our study is that we only used male flies in our experiments. We did this for practical reasons given the size of the experiment, it is clear from many studies, including those using the DGRP lines used in this study, that the phenotypic responses of females of a given genotype often do not match those of males of the same genotype [ 56 , 57 ]. These genotype by sex interactions can complicate our understanding of the genetic basis of phenotypic variation, as polymorphisms that contribute to male variation may differ from those affecting the same phenotype in females.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis in Drosophila Reveals the Genetic Basis of Variation in Age-Specific Physical Performance and Response to ACE Inhibition

Gabrawy

Khosravian

Morcos

et al. 2022

Genes

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Despite impressive results in restoring physical performance in rodent models, treatment with renin–angiotensin system (RAS) inhibitors, such as Lisinopril, have highly mixed results in humans, likely, in part, due to genetic variation in human populations. To date, the genetic determinants of responses to drugs, such as RAS inhibitors, remain unknown. Given the complexity of the relationship between physical traits and genetic background, genomic studies which predict genotype- and age-specific responses to drug treatments in humans or vertebrate animals are difficult. Here, using 126 genetically distinct lines of Drosophila melanogaster, we tested the effects of Lisinopril on age-specific climbing speed and endurance. Our data show that functional response and sensitivity to Lisinopril treatment ranges from significant protection against physical decline to increased weakness depending on genotype and age. Furthermore, genome-wide analyses led to identification of evolutionarily conserved genes in the WNT signaling pathway as being significantly associated with variations in physical performance traits and sensitivity to Lisinopril treatment. Genetic knockdown of genes in the WNT signaling pathway, Axin, frizzled, nemo, and wingless, diminished or abolished the effects of Lisinopril treatment on climbing speed traits. Our results implicate these genes as contributors to the genotype- and age-specific effects of Lisinopril treatment and because they have orthologs in humans, they are potential therapeutic targets for improvement of resiliency. Our approach should be widely applicable for identifying genomic variants that predict age- and sex-dependent responses to any type of pharmaceutical treatment.

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“…Genetic factors have been implicated in spontaneous (i.e., unstimulated) grooming behavior in Drosophila melanogaster (36) and in other drosophilid species (31). Our results demonstrate that this is true for dust-induced grooming as well.…”

Section: Discussionmentioning

confidence: 99%

Variation and variability inDrosophilagrooming behavior

Mueller¹,

Zhang

Carlson³

et al. 2020

Preprint

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Behavioral differences can be observed between species or populations (variation) or between individuals in a genetically similar population (variability). Here, we investigate genetic differences as a possible source of variation and variability in Drosophila grooming. Drosophila grooming behavior confers survival and social benefits. Although the leg movements that constitute the grooming sequence are stereotyped, their order is not fixed. Grooming features of five drosophilid species exposed to a dust irritant were analyzed. Components of grooming behavior were conserved between and within species. However, significant differences in grooming syntax were identified, corresponding both to anterior and posterior grooming actions. Genetic heterogeneity was not found to be related to grooming variability, as melanogaster flies bred to increase genetic heterogeneity did not exhibit increased variability in grooming syntax. Likewise, no relationship between decreased heterogeneity and variability was identified. Finally, individual melanogaster flies were observed on consecutive days to determine the degree of variability of grooming behavior within an individual over time. Individual flies were not found to possess strong, stable grooming traits over several recordings. Additionally, standardization of sensory input using optogenetics did not eliminate grooming variability. In aggregate, these data suggest the importance of sensory inputs and other factors such as life history in grooming variability.

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Genetic Basis of Natural Variation in Spontaneous Grooming inDrosophila melanogaster

Cited by 5 publications

References 49 publications

Variation and Variability in Drosophila Grooming Behavior

Variation and Variability in Drosophila Grooming Behavior

Genome-Wide Analysis in Drosophila Reveals the Genetic Basis of Variation in Age-Specific Physical Performance and Response to ACE Inhibition

Variation and variability inDrosophilagrooming behavior

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