Genetic association analysis reveals significant effect of β-casein A1/A2 loci on production &amp; reproduction traits in Frieswal crossbred cows

Kumar, Adesh; Singh, Rajbir; Chauhan, Anuj; Ilayakumar, K; Kumar, Sushil; Kumar, Amit; Sonwane, Arvind; Kumar, Subodh; Panigrahi, Manjit; Bhushan, Bharat

doi:10.1080/09291016.2019.1571705

Cited by 16 publications

(17 citation statements)

References 31 publications

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“…The remaining 20% of the protein content is the serum protein (Davoodi et al, 2016). Beta casein's gene (CSN2) belongs to the cluster of four casein genes located on chromosome 6 (Ferretti, Leone, & Sgaramella, 1990;Jann, Ceriotti, Caroli, & Erhardt, 2002;Kumar et al, 2019). It is known that beta casein has 12 genetic variants, (A1, A2, A3, B, C, D, E, F, G, H1, H2 and I) (Kumar et al, 2019;Massella et al, 2017;Sebastiani et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Beta casein's gene (CSN2) belongs to the cluster of four casein genes located on chromosome 6 (Ferretti, Leone, & Sgaramella, 1990;Jann, Ceriotti, Caroli, & Erhardt, 2002;Kumar et al, 2019). It is known that beta casein has 12 genetic variants, (A1, A2, A3, B, C, D, E, F, G, H1, H2 and I) (Kumar et al, 2019;Massella et al, 2017;Sebastiani et al, 2020). Among them, the most frequent genetic variants of CSN2 are A1 and A2 (Balteanu, Vlaic, Suteu, & Carsai, 2010;Cieślińska et al, 2012;Dinc, Ozkan, Koban, & Togan, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The A1 variant differs to the A2 at one amino acid of the amino acid chain. More specifically, proline (codon CCT) on the 67th position (A2 variant) has been replaced by histidine (codon CAT) (A1 variant) (Kumar et al, 2019;Miluchová et al, 2014;Massella et al, 2017). This mutation has an effect on the proteolytic digestion of the primary protein structure, leading to the production of different peptides.…”

Section: Introductionmentioning

confidence: 99%

“…Β-casomorphin is a bioactive peptide produced from β-casein variants A1 and has a significant opioid activity (Brooke-Taylor, Dwyer, Woodford, & Kost, 2017;Massella et al, 2017). BCM-7 is a small molecule that can infiltrate blood circulation more easily, and cause various health problems, such as gastrointestinal disorders, insulin-dependent diabetes, atherosclerosis, ischemic heart diseases and sudden infant death syndrome (Brooke-Taylor et al, 2017;Boztepe, Aytekin, & Şahin, 2018;Elliott, Harris, Hill, Bibby, & Wasmuth, 1999;Kumar et al, 2019;McLachlan, 2001;Sun et al, 2003). To our knowledge, there is a lack of genetic polymorphism of b-casein in cows that are bred in Greece.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Polymorphisms of Gene CSN2 of B Casein in Greek Cow Breeds (Holstein) by Restriction Fragment Length Polymorphism

Vougiouklaki¹,

Antonopoulos²,

Allexeli³

et al. 2020

JAS

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The research focused to detect and identify genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows. For this purpose, DNA was extracted from 120 blood samples of cows. The desired region of exon 7 was amplified by PCR, resulting in a 121 bp product. The PCR product was digested by restriction fragment length polymorphism (RFLP) method. The results suggest that the A1A2 genotype prevails over the others. Specifically, of the 120 cattle, 72 showed triple bands of 121 bp, 86 bp, and 35 bp indicating the A1A2 genotype. The 42 cattle showed a single band at 121 bp, indicating that they carried the A1A1 genotype. The remaining 6 showed only two bands of 86 and 35 bps, indicating that they carried the A2A2 genotype. In the total population of heterozygotes A1A2-0.60 were the most frequent, while homozygotes A2A2-0.06 were the least frequent ones. This suggests a slight superiority of allele A-0.65.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Polymorphisms of Gene CSN2 of B Casein in Greek Cow Breeds (Holstein) by Restriction Fragment Length Polymorphism

Vougiouklaki¹,

Antonopoulos²,

Allexeli³

et al. 2020

JAS

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“…In recent years, Beta-casein has been a popular topic of research in the animal breeding and dairy industry as it is a source for high-quality protein, and that bioactive peptide has numerous health implications (Kumar et al 2019). The polymorphic nature of beta-casein about milk production quality and composition has led in assessing the allelic distribution of beta-casein locus as a potential dairy trait marker (Dai et al 2016).…”

Section: Introductionmentioning

confidence: 99%

PCR Based Genotyping of Lulu Cattle of Nepal for A1, A2 Type Beta-caseins

Gorkhali¹,

Sherpa²,

Budhathoki³

et al. 2020

J. Nep. Agric. Res. Counc.

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Lulu is an indigenous breed of cattle (Bos taurus) found in high altitude regions of western Nepal. Population of Lulu cattle has been declining due to introgression with other exotic breeds to increase milk productivity. Here we aimed at finding potential approach for conserving Lulu cattle and its assets by studying the milk contents and investigating which variant of beta-casein protein is present in this breed. Beta caseins are an abundant protein in cow milk with A1 and A2 being the most common genetic variants of this protein. Consumption of A1 type of milk has numerous health-related complications whereas A2 type of milk has numerous human health promoting factors. We used restriction fragment length polymorphism (RFLP) for determining the A1 and A2 variant of beta casein in Lulu cattle. For performing DNA extraction, we collected (n = 18) blood samples of Lulu from Mustang and (n=17) Nepal Agriculture research council farm. The amplified fragments in 3% agarose at 251bp and 213bp respectively confirmed the presence of both A1 and A2 gene in Lulu; however, A2 was of greater abundance. Our study indicated that Lulu has A2 variant of beta-casein predominantly. The gene frequency of A1A1 is 0, A1A2 is 0.06 and A2A2 is 0.94. We further found that the allele frequency of A1 and A2 is 0.03 and 0.97 respectively. We designed special primer for sequencing CSN2 genes since A2 type beta casein gene was predominantly seen on Lulu. The sequencing result further supports our RFLP result as most of our samples have "C" nucleotide SNP in amplified CSN2 gene sequence. The Chi-square value of the current study is 0.04 which supports Hardy-Weinberg equilibrium inferring that Lulu cattle are still in the pure state, where there is no genetic introgression with the exotic breed for the sake of improvement of productivity.

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Casein (CSN) gene variants and parity affect the milk protein traits in crossbred (Bos taurus x Bos indicus) cows in sub-tropical climate

Mohan

Kumar

Khan

et al. 2021

Trop Anim Health Prod

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Genetic association analysis reveals significant effect of β-casein A1/A2 loci on production & reproduction traits in Frieswal crossbred cows

Cited by 16 publications

References 31 publications

Identification of Polymorphisms of Gene CSN2 of B Casein in Greek Cow Breeds (Holstein) by Restriction Fragment Length Polymorphism

Identification of Polymorphisms of Gene CSN2 of B Casein in Greek Cow Breeds (Holstein) by Restriction Fragment Length Polymorphism

PCR Based Genotyping of Lulu Cattle of Nepal for A1, A2 Type Beta-caseins

Casein (CSN) gene variants and parity affect the milk protein traits in crossbred (Bos taurus x Bos indicus) cows in sub-tropical climate

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