Genetic and Phytochemical Diversity Assessment Among Eleven<i>Hypericum</i>Accessions via AFLP and HPLC Analyses

Aziz, Naseer; Sauvé, Rémy; Long, Deborah M.; Cherry, Mary

doi:10.1300/j044v12n01_09

Cited by 11 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, a clear specification of the variety or cultivar evaluated is not always provided in the literature, making the results unfortunately prone to misinterpretations and misleading results. A suitable characterization of accessions with genetic markers would be highly valuable; various genetic fingerprinting methods are becoming available and their widespread use should be endorsed [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 ]. Moreover, careful and detailed description of the habitat of collection for wild accessions would be extremely useful to adequately interpret data obtained, but unfortunately is rarely provided.…”

Section: Genetic Variability: Chemotaxonomy Phenotypic Variationsmentioning

confidence: 99%

Factors Affecting Polyphenol Biosynthesis in Wild and Field Grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae)

2009

View full text Add to dashboard Cite

The increasing diffusion of herbal products is posing new questions: why are products so often different in their composition and efficacy? Which approach is more suitable to increase the biochemical productivity of medicinal plants with large-scale, low-cost solutions? Can the phytochemical profile of a medicinal plant be modulated in order to increase the accumulation of its most valuable constituents? Will polyphenol-rich medicinal crops ever be traded as commodities? Providing a proactive answer to such questions is an extremely hard task, due to the large number of variables involved: intraspecific chemodiversity, plant breeding, ontogenetic stage, post-harvest handling, biotic and abiotic factors, to name but a few. An ideal path in this direction should include the definition of optimum pre-harvesting and post-harvesting conditions and the availability of specific Good Agricultural Practices centered on secondary metabolism enhancement. The first steps to be taken are undoubtedly the evaluation and the organization of scattered data regarding the diverse factors involved in the optimization of medicinal plant cultivation, in order to provide an interdisciplinary overview of main possibilities, weaknesses and drawbacks. This review is intended to be a synopsis of the knowledge on this regard focused on Hypericum perforatum L. (Hypericaceae/Guttiferae) secondary metabolites of phenolic origin, with the aim to provide a reference and suggest an evolution towards the maximization of St. John's Wort bioactive constituents. Factors considered emerged not only from in-field agronomic results, but also from physiological, genetical, biotic, abiotic and phytochemical data that could be scaled up to the application level. To increase quality for final beneficiaries, growers’ profits and ultimately transform phenolic-rich medicinal crops into commodities, the emerging trend suggests an integrated and synergic approach. Agronomy and genetics will need to develop their breeding strategies taking account of the suggestions of phytochemistry, biochemistry, pharmacognosy and pharmacology, without losing sight of the economic balance of the production.

show abstract

Section: Genetic Variability: Chemotaxonomy Phenotypic Variationsmentioning

confidence: 99%

Factors Affecting Polyphenol Biosynthesis in Wild and Field Grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae)

2009

View full text Add to dashboard Cite

show abstract

“…Quality control involved with the production and distribution of phytopharmaceutical medicines has not been highly regulated with respect to species of plants being used in the preparation of commercial products and the concentration of bioactive compounds. Moreover, the technology available for identification of H. perforatum plant material in commercially available products is not standardized and thus variation between products is an issue [14]. Because of the importance of H. perforatum to the phytopharmaceutical industry, it is important to develop a reliable marker system that can be used to affordably and accurately identify plant material purported to be H. perforatum in order to aid producers while protecting consumers from potentially adulterated products.…”

Section: Introductionmentioning

confidence: 99%

“…While other studies have utilized a molecular approach to place H. perforatum within a phylogenetic framework [14], [20], [29], [30], this is the first study placing an emphasis on the relationships and diversity between both wild and cultivated accessions of H. perforatum, within the overall phylogenetic framework of the genus Hypericum. This study demonstrates that there is a great deal of genetic diversity among Hypericum species as well as within H. perforatum, and that this diversity is structured phylogenetically and geographically.…”

mentioning

confidence: 99%

Genetic Diversity in Hypericum and AFLP Markers for Species-Specific Identification of H. perforatum L.

Percifield¹,

Hawkins²,

McCoy³

et al. 2007

Planta Med

View full text Add to dashboard Cite

One of the top-selling medicinal products worldwide is Hypericum perforatum (St. John's Wort). Despite its cosmopolitan distribution and utilization, little is known regarding the relationship of the bioactive compounds in H. perforatum to the plants from which they are purportedly derived. In this study, amplified fragment length polymorphism (AFLP) analysis of 56 Hypericum accessions, representing 11 species, was conducted to gain a better understanding of diversity within Hypericum species, especially within cultivated accessions of H. perforatum, and to establish a molecular methodology that will provide breeders and regulators with a simple, affordable, and accurate tool with which to identify purported H. perforatum material. Utilizing four primer combinations, a total of 298 polymorphic markers were generated, of which 17 were present in all H. perforatum accessions and 2 were specific to only H. perforatum. This study demonstrates that AFLP can be utilized not only to determine the relationships of closely related Hypericum accessions, but as a tool to authenticate material in herbal remedies through the use of genetic fingerprinting.

show abstract

“…As a matter of fact, hypericin was never detected in the whole plant as well (Kitanov, 2001 ). Regarding hyperforin, other studies confirmed its absence from H. androsaemum (Aziz et al, 2006 ).…”

Section: Resultsmentioning

confidence: 80%

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

et al. 2016

View full text Add to dashboard Cite

Hypericum androsaemum, also known as Tutsan, is a small evergreen shrub common in the Mediterranean basin where it is traditionally used as diuretic and hepatoprotective herbal drug. This plant possesses the peculiarity to produce fleshy and berry-like fruits that ripen from red to shiny black. In the present work, the chemical constituents of methanolic extracts and infusions of red and black fruits were analyzed by HPLC, and correlated with their antioxidant properties which were evaluated by the DPPH, β-Carotene/linoleic acid, and hypochlorous acid tests. In addition, the red pigment of the fruit was isolated by column chromatography and structurally elucidated by NMR. Results showed that H. androsaemum fruits contain high amounts of shikimic and chlorogenic acids, while their color was given by a tetraoxygenated-type xanthone, reported for the first time in Hypericum species. The red berries infusion gave the highest content of total phenolic compounds, DPPH, and hypochlorous acid scavenging activity, and β-carotene bleaching. Cytotoxicity of the berries extracts on three human tumor cell lines (malignant melanoma, breast adenocarcinoma, and colon carcinoma) was evaluated by MTT assay, and relevant inhibition on colon carcinoma cells (IC50 value of 8.4 μg/mL) was found. Finally, the effects of red berries extract on the immune system were evaluated by peripheral blood mononuclear cell (PBMC) proliferation assay that revealed a strong stimulation on lymphocytes at low doses (0.4–6 μg/mL).

show abstract

Genetic and Phytochemical Diversity Assessment Among ElevenHypericumAccessions via AFLP and HPLC Analyses

Cited by 11 publications

References 12 publications

Factors Affecting Polyphenol Biosynthesis in Wild and Field Grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae)

Factors Affecting Polyphenol Biosynthesis in Wild and Field Grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae)

Genetic Diversity in Hypericum and AFLP Markers for Species-Specific Identification of H. perforatum L.

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

Contact Info

Product

Resources

About