Genetic Diversity in <i>Hypericum</i> and AFLP Markers for Species-Specific Identification of <i>H. perforatum</i> L.

Percifield, Ryan; Hawkins, Jennifer; McCoy, Joe-Ann; Widrlechner, Mark P.; Wendel, Jonathan F.

doi:10.1055/s-2007-993749

Cited by 33 publications

(33 citation statements)

References 24 publications

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“…Additionally, the contribution of 58% of the total amount of molecular genetic diversity observed by among-population difference is indicative of low levels of gene flow between populations. High among-population variation was previously reported in Hypericum species by Percifield et al (2007), Pilepić et al (2008), and Farooq et al (2014). High differentiation among populations is mostly coupled with limited gene flow among them.…”

Section: Discussionmentioning

confidence: 59%

“…Molecular analyses revealed that all the ecotypes were polyclonal, not being dominated by a single genotype, and characterized by different levels of differentiation among multilocus genotypes. Percifield et al (2007) performed AFLP analysis of 56 Hypericum accessions, representing 11 species, to gain a better understanding of diversity within Hypericum species, especially within cultivated accessions of H. perforatum, and to establish a molecular methodology that will provide breeders and regulators with a simple, affordable, and accurate tool with which to identify purported H. perforatum material.…”

Section: Introductionmentioning

confidence: 99%

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Transferability of barley retrotransposon primers to analyze geneticstructure in Iranian Hypericum perforatum L. populations

Mamaghani¹,

Mohammadi²,

Aharizad³

2015

Turk J Bot

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Transferability of barley retrotransposon primers was investigated to analyze population structure in St. John's wort (Hypericum perforatum L.) based on inter-retrotransposon amplified polymorphism (IRAP). Seven long terminal repeat (LTR) retrotransposon primers derived from the barley genome were used to detect genetic polymorphism in eight Iranian populations and three cultivars (Helos, New Stem, and Topaz) of H. perforatum based on IRAP analysis. Nine possible LTR primers/primer combinations successfully amplified fragments from the H. perforatum genome. In total, 311 bands of 100-3000 base pairs were amplified, of which 244 were polymorphic. The number of polymorphic fragments ranged from 10 (Nikita/5′LTR-2) to 57 (3′LTR), with an average of 27.11. Principal coordinate analysis (PCoA) could clearly differentiate samples of wild populations and cultivars. Based on analysis of molecular variance (AMOVA), among populations variance explained 58% of total molecular variation. This study demonstrates that IRAP markers can be utilized not only to determine the relationships of Hypericum populations and cultivars, but also as a tool for selection of suitable populations for breeding programs.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Transferability of barley retrotransposon primers to analyze geneticstructure in Iranian Hypericum perforatum L. populations

Mamaghani¹,

Mohammadi²,

Aharizad³

2015

Turk J Bot

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“…Thus, AFLP is a useful method for precise identification of genotype within a short period of time. This method also proves to be very important in plant taxonomy for species-specific identification (Lin et al, 1996, soybean;Waugh et al, 1997, barley;Degani et al, 2001, strawberry;Saunders et al, 1999, 2001, marijuana andopium, respectively;Percifield et al, 2007, Hypericum perforatum L.; Misra et al, 2010, Swertia sp).…”

Section: Introductionmentioning

confidence: 99%

Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

Ghosh¹,

Majumder²,

Mandi³

2011

Genet. Mol. Res.

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ABSTRACT. The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic properties, is Zingiber officinale (garden ginger), which is often adulterated with other lesspotent Zingiber sp. There is an existing demand in the herbal drug industry for an authentication system for the Zingiber sp in order to facilitate their commercial use as genuine phytoceuticals. To this end, we used amplified fragment length polymorphism (AFLP) to produce DNA fingerprints for three Zingiber species. Sixteen collections (six of Z. officinale, five of Z. montanum, and five of Z. zerumbet) were used in the study. Seven selective primer pairs were found to be useful for all the accessions. A total of 837 fragments were produced by these primer pairs. Species-specific markers were identified for all three Zingiber species (91 for Z. officinale, 82 for Z. montanum, and 55 for Z. zerumbet). The dendogram analysis generated from AFLP patterns showed that Z. montanum and Z. zerumbet are phylogenetically closer to each other than to Z. officinale. The AFLP fingerprints of the Zingiber species could be AFLP markers for identification of three Zingiber species used to authenticate Zingiber sp-derived drugs and to resolve adulterationrelated problems faced by the commercial users of these herbs.

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“…There are a variety of DNA marker techniques, such as random amplified polymorphic sequence (RAPD) (Juchum et al, 2007;Ro et al, 2007), amplified restriction fragment polymorphism (AFLP) (Percifield, 2007;Yuan et al, 2007) and microsatellite markers (SSR) (Legesse et al, 2007;Tommasini et al, 2003). They are widely applied in species or varieties identification, evaluation of genetic diversity, but few reports are available on systematically comparative analysis of two kinds of testing approaches, such as morphological method and molecular marker method.…”

Section: Introductionmentioning

confidence: 99%

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

Liu¹,

Liu²,

Chen³

et al. 2011

JAS

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The test of distinctiveness, uniformity, and stability (DUS) is a necessary step for variety identification and new variety application. The objective of this study is to provide molecular marker-assisted approach combined with morphological trait-based testing for more convenient and fast DUS test and identification of varieties. Eighteen pairs of SRAP markers and 40 morphological traits for DUS test were applied for genetic diversity analysis of 50 lettuce (Lactuca sativa) varieties. Average polymorphism information content (PIC) of the SRAP markers was 0.80, ranging from 0.39 to 0.97. Cluster analysis using UPGMA of the band patterns amplified by SRAP marker and morphological trait-based clustering separated the varieties into three groups. The correlation coefficient of SRAP marker and morphological traits was 0.5455 reflecting that the two clustering results shared some similarity and consistence. It revealed that the combination of both SRAP marker and morphological trait analysis is more conducive to proper identification and classification of plant varieties, which will undoubtedly bring an alternative choice to DUS testing of plant new varieties and conservation of plant germplasm.

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Genetic Diversity in Hypericum and AFLP Markers for Species-Specific Identification of H. perforatum L.

Cited by 33 publications

References 24 publications

Transferability of barley retrotransposon primers to analyze geneticstructure in Iranian Hypericum perforatum L. populations

Transferability of barley retrotransposon primers to analyze geneticstructure in Iranian Hypericum perforatum L. populations

Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

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