Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice

Sanchez, Alma; Ilag, Lawrence L.; Yang, Donglei; Brar, D. S.; Ausubel, Frederick M.; Khush, G. S.; Yano, Masahiro; Sasaki, Takuji; Li, Z.; Huang, Ning

doi:10.1007/s001220051163

Cited by 56 publications

(31 citation statements)

References 15 publications

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“…This feature also facilitates map-based cloning of genes responsible for specific phenotypes (Danesh et al 1998; Nam et al 1999; Patocchi et al 1999; Sanchez et al 1999). …”

Section: Chapter 1 Overviewmentioning

confidence: 99%

“…Consequently, many interesting and important genes have been isolated (Wang et al 1996;Nakamura et al 1997;Yang et al 1997;Cai et al 1998; Danesh et al 1998; Yang et al 1998;Folkertsma et al 1999; Moullet et al 1999; Nam et al 1999; Patocchi et al 1999; Salimath and Bhattacharyya 1999; Sanchez et al 1999). High-throughput physical mapping already has resulted in the construction of BAC contigs encompassing entire chromosomes and/or complete chromosome sets (Mozo et al 1999).…”

Section: Chapter 1 Overviewmentioning

confidence: 99%

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Diversity and abundance of the abnormal chromosome 10 meiotic drive complex in Zea mays

et al. 2013

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1) CHAPTER 7(a) Recent experiments have shown that the Percoll gradient step is not necessary to get nuclear preparations of sufficient quality. Thus this step has been deleted. (b) EGTA and L-lysine have been made standard components of MEB and MPDB solutions. These compounds greatly reduce DNA damage caused by endogenous nucleases. 2) CHAPTERS 7, 9, and 10 -PVP is no longer added to the lysis buffer or wash buffers (WB-A, WB-B, and WB-C). PVP often precipitates out of solution to form a brown gel in which the agarose/nuclei plugs may get stuck. Lysis buffer is now made 6 mM EGTA and 200 mM L-lysine. 3) CHAPTER 13 -We no longer recommend the use of GELase to isolate BAC insert DNA from plugs. Our experience suggests that the DNA may be damaged by GELase. Electroelution has proven the most effective means of obtaining clonable DNA from agarose plugs. 4) CHAPTER 9 -Mathematical errors in Table 9.1 have been corrected. ABSTRACTBacterial artificial chromosome (BAC) libraries have become invaluable tools in plant genetic research. However, it is difficult for new practitioners to create plant BAC libraries de novo because published protocols are not particularly detailed, and plant cells possess features that make isolation of clean, high molecular weight DNA troublesome. In this document we present an illustrated, step-by-step protocol for constructing plant BAC libraries. This protocol is sufficiently detailed to be of use to both new and experienced investigators. We hope that by reducing the obstacles to BAC cloning in plants, we will foster new and accelerated progress in plant genomics.

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“…This feature also facilitates map-based cloning of genes responsible for specific phenotypes (Danesh et al 1998; Nam et al 1999; Patocchi et al 1999; Sanchez et al 1999). …”

Section: Chapter 1 Overviewmentioning

confidence: 99%

Section: Chapter 1 Overviewmentioning

confidence: 99%

Diversity and abundance of the abnormal chromosome 10 meiotic drive complex in Zea mays

et al. 2013

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“…oryzae strains used in this study included PXO86, PXO79, PXO71, PXO112, PXO99, PXO280, PXO145, PXO87 and PXO124 from the Philippines and ZHE173 from Zhejiang Province of China. Inoculation was performed by the leaf-clipping method described by Kauffman et al [7] and Sanchez et al [8] . Briefly, at the booting stage (about 40 days after transplanting), the uppermost fully expanded leaves were clipped with scissors about 10 mm from the tip then dipped into the inoculum.…”

Section: Pathogen Inoculationmentioning

confidence: 99%

“…Briefly, at the booting stage (about 40 days after transplanting), the uppermost fully expanded leaves were clipped with scissors about 10 mm from the tip then dipped into the inoculum. The strains used for inoculation were grown for 48 h on potato semisynthetic agar [8] and the density was adjusted to 10 9 CFU$mL -1 when suspended in sterile water. Each strain was used to inoculate 4 leaves on each of 10 plants.…”

Section: Pathogen Inoculationmentioning

confidence: 99%

Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana

Wang¹,

Chen²,

Zhou³

et al. 2015

Front. Agr. Sci. Eng.

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Y73 is a progeny of asymmetric somatic hybridization between Oryza sativa cv. Dalixiang and the wild rice species Oryza meyeriana. Inoculation with a range of strains of Xanthomonas oryzae pv. oryzae showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F 2 population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F 2 population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

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“…The most common BAC vector is pBeloBAC11 [9,15]. [18,19]. Many important genes were cloned and functionally confirmed [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Achievement, Challenge and Future Perspective of BAC Library Technology with a Focus on its Application to Soybean Genomics Researches

Wu¹,

Xia²

2015

Clon Transgen

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Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice

Cited by 56 publications

References 15 publications

Diversity and abundance of the abnormal chromosome 10 meiotic drive complex in Zea mays

Diversity and abundance of the abnormal chromosome 10 meiotic drive complex in Zea mays

Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana

Achievement, Challenge and Future Perspective of BAC Library Technology with a Focus on its Application to Soybean Genomics Researches

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