Genetic and histological characterization of a novel recessive genic male sterile line of Brassica napus derived from a cross with Capsella bursa-pastoris

Abstract

In a previously made cross Brassica napus cv. Oro (2n = 38) 9 Capsella bursa-pastoris (2n = 4x = 32), one F 1 hybrid with 2n = 38 was totally male sterile. The hybrid contained no complete chromosomes from C. bursa-pastoris, but some specific AFLP (amplified fragment length polymorphism) bands of C. bursa-pastoris were detected. The hybrid was morphologically quite similar to 'Oro' except for smaller flowers with rudimentary stamens but normal pistils, and showed good seed-set after pollination by 'Oro' and other B. napus cultivars. The fertility segregation ratios (3:1, 1:1) in its progenies indicated that the male sterility was controlled by a single recessive gene. In the pollen mother cells of the male sterile hybrid, chromosome pairing and segregation were normal. Histological sectioning of its anthers showed that the tapetum was multiple layers and was hypertrophic from the stage of sporogenic cells, and that the tetrads were compressed by the vacuolated and disaggregated tapetum and no mature pollen grains were formed in anther sacs, thus resulting in male sterility. The possible mechanisms for the production of the male sterile hybrid and its potential in breeding are discussed.

The Frustration with Utilization: Why Have Improvements in Internal Phosphorus Utilization Efficiency in Crops Remained so Elusive?

“…Several similar studies have reported genotypic differences in PUE and PAE across multiple crop species (see recent reviews by Shenoy and Kalagudi, 2005; Wang et al, 2010). In addition, a limited number of studies have reported Quantitative Trait Loci (QTL) for PUE (Wissuwa et al, 1998; Su et al, 2006; Chen et al, 2009). …”

Quantitative trait loci analysis of stem strength and related traits in soybean

“…The PCR reaction was performed at 94°C for 5 min, followed by 35 cycles of 94°C for 30 s, 47°C for 30 s and 72°C for 45 s, with a final extension for 10 min at 72°C. The PCR products were analyzed by electrophoresis in 6% polyacrylamide gels, and DNA fragments were visualized by silver staining (Bassam et al 1991;Chen et al 2009). …”

Molecular regulation ofBna1205ams1required for male fertility and development of a recessive genic male-sterility system inBrassica napus

“…The epistatic suppressor gene was widespread in Brassiceae. A tri-genetic inheritance model, later proposed to interpret sterility inheritance in 9012AB, suggests that male sterility is controlled by the interaction of two recessive male-sterile genes (Bnms3 and Bnms4) with an epistatic suppressor gene (BnRf or BnEsp) (Chen et al, 1998). In other reports on B. napus (Song et al, 2006) and B.…”

“…The copyright holder for this preprint this version posted February 26, 2024. ; https://doi.org/10.1101/2024.02.25.581914 doi: bioRxiv preprint male-sterile genes (Bnms3 and Bnms4) with an epistatic suppressor gene (BnRf or BnEsp) (Chen et al, 1998). In other reports on B. napus (Song et al, 2006) andB.…”