Molecular regulation ofBna1205ams1required for male fertility and development of a recessive genic male-sterility system inBrassica napus

Abstract

Through the comprehensive use of two pollination control systems of cytoplasmic male sterility (CMS) and genic male sterility (GMS), the rapeseed yield was increased by more than 20%. However, more hybrid production systems and detailed mechanisms underlying male sterility are required. Here, we reported a novel two-line hybrid production system of 1205A for GMS and also investigated the underlying mechanism for male sterility. Five co-segregated kompetitive allele specific PCR (KASP) markers were developed and validated, which could be used for transferring the male sterility trait into externalB. napusbreeding lines and developing further two-line hybrid production systems of GMS. Inheritance studies detected one gene locus ofBna1205ams1for regulating the male sterility of 1205A. As a potential candidate gene ofBna1205ams1,BnaC03g27700Dwas fine mapped and narrowed down to a 181.47 kb region on chrC03 and validated by functional analysis. The mutation ofBnaC03g27700Din 1205A resulted in large metabolic fluctuations, most of which were involved in aborted tapetal PCD, which could lead to reduced pollen fertility with abnormal pollen exine. The developed new GMS line of 1205AB provided us with the opportunity to identify a new male sterility gene ofBnaC03g27700DinB. napus. The study ofBnaC03g27700Daims to renew the annotation of the gene and provide new resources for basic research on the genetic control of male sterility.