Genetic and genomic research for the development of an efficient breeding system in heterostylous self-incompatible common buckwheat (Fagopyrum esculentum)

Matsui, Kenichi; Yasui, Yukihiko

doi:10.1007/s00122-020-03572-6

Cited by 21 publications

(28 citation statements)

References 101 publications

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“…This SI system is controlled by a single genetic locus, S; thrum is heterozygous (Ss) and pin is homozygous recessive (ss). We developed SC buckwheat lines from an interspecific cross between common buckwheat, F. esculentum, and a self-compatible wild relative, F. homotropicum [10]. The SC line has a long homostyle (LH) controlled by a single allele, S h , in the dominance relationship S > S h > s [39].…”

Section: Plant Materialsmentioning

confidence: 99%

“…We developed four PHS-tolerant cultivars/breeding lines-'Harunoibuki', 'NARO-FE-1' (NF1), 'Kyukei 28' (KY28), and 'Kyukei 29' (KY29)-by mass selection of low-PHS individuals [8,9]. To clarify the inheritance of the PHS tolerance of these lines, we performed genetic analysis using segregating populations derived from crosses between KY28 or KY29 and the self-compatible PHS-susceptible 'Kyukei SC7' (KSC7) [8]; KSC7 was developed by the introduction of the self-compatibility (SC) allele of a wild relative, F. homotropicum [10]. In the F 2 progeny derived from KY28 × KSC7, the segregation pattern of the frequency of PHS suggested that several recessive genes regulate PHS tolerance in KY28.…”

Section: Introductionmentioning

confidence: 99%

“…Because buckwheat is an outcrossing plant, it is difficult to fix favorable traits such as PHS tolerance. Although markerassisted selection (MAS) is an efficient way to do so [10], quantitative trait locus (QTL) and selection markers for PHS tolerance in buckwheat have not been reported until now.…”

Section: Introductionmentioning

confidence: 99%

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Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

et al. 2021

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Background Common buckwheat (2n = 2x = 16) is an outcrossing pseudocereal whose seeds contain abundant nutrients and potential antioxidants. As these beneficial compounds are damaged by preharvest sprouting (PHS) and PHS is likely to increase with global warming, it is important to find efficient ways to develop new PHS-tolerant lines. However, genetic loci and selection markers associated with PHS in buckwheat have not been reported. Results By next-generation sequencing (NGS) of whole-genome of parental lines, we developed a genome-wide set of 300 markers. By NGS- based bulked segregant analysis (NGS-BSA), we developed 100 markers linked to PHS tolerance. To confirm the effectiveness of marker development from NGS-BSA data, we developed 100 markers linked to the self-compatibility (SC) trait from previous NGS-BSA data. Using these markers, we developed genetic maps with AmpliSeq technology, which can quickly detect polymorphisms by amplicon-based multiplex targeted NGS, and performed quantitative trait locus (QTL) analysis for PHS tolerance in combination with NGS-BSA. QTL analysis detected two major and two minor QTLs for PHS tolerance in a segregating population developed from a cross between the PHS-tolerant ‘Kyukei 29’ and the self-compatible susceptible ‘Kyukei SC7’. We found different major and minor QTLs in other segregating populations developed from the PHS-tolerant lines ‘Kyukei 28’ and ‘NARO-FE-1’. Candidate markers linked to PHS developed by NGS-BSA were located near these QTL regions. We also investigated the effectiveness of markers linked to these QTLs for selection of PHS-tolerant lines among other segregating populations. Conclusions We efficiently developed genetic maps using a method combined with AmpliSeq technology and NGS-BSA, and detected QTLs associated with preharvest sprouting tolerance in common buckwheat. This is the first report to identify QTLs for PHS tolerance in buckwheat. Our marker development system will accelerate genetic research and breeding in common buckwheat.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

et al. 2021

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“…Breeding of common buckwheat is difficult because of a single gene complex S-locus, which controls self-incompatibility, as it is tied to favorable weather conditions during flowering, which influence the presence of pollinators [78][79][80]. An optimally designed breeding program includes an appropriate initial pin/thrum ratio of parent plants in favor of thrum plants.…”

Section: Buckwheat Breeding: Challenges and Prospects For The Futurementioning

confidence: 99%

Breeding Buckwheat for Increased Levels of Rutin, Quercetin and Other Bioactive Compounds with Potential Antiviral Effects

Luthar

Germ

Likar

et al. 2020

Plants

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Common buckwheat (Fagopyrum esculentum Moench) and Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) are sources of many bioactive compounds, such as rutin, quercetin, emodin, fagopyrin and other (poly)phenolics. In damaged or milled grain under wet conditions, most of the rutin in common and Tartary buckwheat is degraded to quercetin by rutin-degrading enzymes (e.g., rutinosidase). From Tartary buckwheat varieties with low rutinosidase activity it is possible to prepare foods with high levels of rutin, with the preserved initial levels in the grain. The quercetin from rutin degradation in Tartary buckwheat grain is responsible in part for inhibition of α-glucosidase in the intestine, which helps to maintain normal glucose levels in the blood. Rutin and emodin have the potential for antiviral effects. Grain embryos are rich in rutin, so breeding buckwheat with the aim of producing larger embryos may be a promising strategy to increase the levels of rutin in common and Tartary buckwheat grain, and hence to improve its nutritional value.

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“…The grain contains abundant nutrients and bioactive compounds, and much research has been done to improve the yield and eliminate the proteins that cause allergic reactions in some people. Matsui and Yasui (2020) summarized recent developments of buckwheat breeding materials, tools, and methods, including the development of self-compatible lines, genetic maps, and genome database. They also presented strategy for efficient breeding and emerging breeding methods for high-value lines.…”

mentioning

confidence: 99%

Crop genetics research in Asia: improving food security and nutrition

Zhang

Xia

et al. 2020

Theor Appl Genet

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Genetic and genomic research for the development of an efficient breeding system in heterostylous self-incompatible common buckwheat (Fagopyrum esculentum)

Cited by 21 publications

References 101 publications

Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Breeding Buckwheat for Increased Levels of Rutin, Quercetin and Other Bioactive Compounds with Potential Antiviral Effects

Crop genetics research in Asia: improving food security and nutrition

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