Genetic and epigenetic mechanisms in the aetiology of orofacial clefts

Cruz, Lucas Alvizi

doi:10.11606/t.41.2018.tde-12122017-172943

Cited by 1 publication

(1 citation statement)

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“…genetic factors, deficiency of vitamin B6, vitamin B12, folic acid and iron, as well as other factors, such as environmental exposure like organic solvents, air pollution, cigarettes and agricultural chemicals. 4,5 Environmental exposure may have an effect on epigenetic mechanisms, particularly deoxyribonucleic acid (DNA) or histone methylation. 6 Methylation of DNA has an effect of changing gene activity without altering gene sequences and can be inherited.…”

Section: Introductionmentioning

confidence: 99%

Difference in DNA Methylation between Cleft Lip and Cleft Lip and Palate

Ruby Riana Asparini,

David S. Perdanakusuma,

Retno Handajani

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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It is suspected that environmental exposure to non-syndromic oral clefts, which includes cleft lip (CL), cleft lip and palate (CLP) has an effect on epigenetic mechanisms, particularly deoxyribonucleic acid (DNA) methylation. DNA methylation will be expressed during facial morphogenesis and have an impact on facial development. This study aimed to observe differences in DNA methylation between CL and CLP, between CL mothers and CLP mothers, and correlation between CL and CL mothers, CLP and CLP mothers. This observational study used a sample of 13 patients diagnosed with CL and 14 patients diagnosed with CLP and their respective mothers. The test was performed using ELISA MethylFlashTM Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric).The median DNA methylation at CLP was 1.92 (0.23 -14.07) and CL was 1.71 (0.08 -8.47) (p 0.752 > 0.05)). Median DNA methylation in CLP mothers was 0.997 (0.03 -6.14) and in CL mothers 0.72 (0.23 -6.16) (p 0.798 (p> 0.05). Correlation test for DNA methylation of CLP with CLP mother r = -0.259 and (p = 0.394 >0.05). Correlation test for DNA methylation of CL patients with CL mothers revealed r = -0.492 and (p = 0.087 > 0.05).The results of this study showed no difference in methylation between CL and CLP. This study found that DNA methylation between CL mothers and CLP mothers was relatively the same. This study also found no correlation between DNA methylation of CL and CL mothers, and between CLP and CLP mothers.

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Section: Introductionmentioning

confidence: 99%