Genetic and cellular analysis of cross-incompatibility in Zea mays

Lu, Yongxian; Kermicle, Jerry L.; Mulenga, Evans

doi:10.1007/s00497-013-0236-5

Cited by 32 publications

(63 citation statements)

References 34 publications

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“…Lu et al . observed that in silk carrying the Tcb , pollen tubes had clustered callose plugs and their growth was slower in comparison to pollen tubes of compatible crosses 37 . Such crossing incompatibility may also exist between commercial maize and Spanish teosinte.…”

Section: Discussionmentioning

confidence: 98%

Teosinte in Europe – Searching for the Origin of a Novel Weed

Trtikova

Lohn

Binimelis

et al. 2017

Sci Rep

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A novel weed has recently emerged, causing serious agronomic damage in one of the most important maize-growing regions of Western Europe, the Northern Provinces of Spain. The weed has morphological similarities to a wild relative of maize and has generally been referred to as teosinte. However, the identity, origin or genetic composition of ‘Spanish teosinte’ was unknown. Here, we present a genome-wide analysis of single-nucleotide polymorphism (SNP) data for Spanish teosinte, sympatric populations of cultivated maize and samples of reference teosinte taxa. Our data are complemented with previously published SNP datasets of cultivated maize and two Mexican teosinte subspecies. Our analyses reveal that Spanish teosinte does not group with any of the currently recognized teosinte taxa. Based on Bayesian clustering analysis and hybridization simulations, we infer that Spanish teosinte is of admixed origin, most likely involving Zea mays ssp. mexicana as one parental taxon, and an unidentified cultivated maize variety as the other. Analyses of plants grown from seeds collected in Spanish maize fields and experimental crosses under controlled conditions reveal that hybridization does occur between Spanish teosinte and cultivated maize in Spain, and that current hybridization is asymmetric, favouring the introgression of Spanish teosinte into cultivated maize, rather than vice versa.

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Section: Discussionmentioning

confidence: 98%

Teosinte in Europe – Searching for the Origin of a Novel Weed

Trtikova

Lohn

Binimelis

et al. 2017

Sci Rep

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“…Collections of teosinte of both mexicana and parviglumis subspecies from the central Mexican plateau carry Tcb1-s ( 6 ). Tcb1-s confers to females the ability to block fertilization by maize ( tcb1 type) pollen by restricting pollen tube growth ( 7 ). In the reciprocal cross, teosinte pollen is able to fertilize maize, although poorly when in competition with maize pollen ( 3 ).…”

Section: Main Textmentioning

confidence: 99%

“…The male and female functions of Tcb1-s are tightly linked but separable by recombination ( 7 ). Thus, there are four functional classes at this locus (Table S1 for gene content and origin): Tcb1-s has both functional male and female genes, Tcb1-m has only the functional male gene ( 6, 7 ), Tcb1-f has only the functional female gene, and the tcb1 haplotype found in almost all maize lines has neither of the two functional genes. In teosinte, Tcb1-f activity in the silks prevents fertilization by maize (tcb1) pollen, while Tcb1-m activity in pollen enables fertilization of Tcb1-f females ( 7 ).…”

Section: Main Textmentioning

confidence: 99%

“…Mixed pollination confirmed this is a Tcb1-male only plant (Fig.S1). Additionally, two independent Tcb1-s lines were isolated in which the barrier gradually lost the strength during ten generations of backcrossing to maize and selection for Tcb1-male function ( 7 ). These two lines were named as tcb1-f::silent lineage1 (tcb1-f::sl1) and tcb1-f::silent lineage2 (tcb1-f::sl2) , based on the “progressive” manner of the barrier weakening.…”

Section: Main Textmentioning

confidence: 99%

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A Silk-Expressed Pectin Methylesterase Confers Cross-Incompatibility Between Wild and Domesticated Strains of Zea mays

et al. 2019

Preprint

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12Despite being members of the same species, some strains of wild teosinte maintain themselves as 13 a distinct breeding population by blocking fertilization by pollen from neighboring maize plants. 14 These teosinte strains may be in the process of evolving into a separate species, since reproductive 15 barriers that block gene flow are critical components in speciation. This trait is conferred by the 16Teosinte crossing barrier1-s (Tcb1-s) haplotype, making Tcb1 a speciation gene candidate. Tcb1-17 s contains a female gene that blocks non-self-type pollen and a male function that enables self-18 type pollen to overcome that block. The Tcb1-female gene encodes a Pectin Methylesterase, 19 implying that modification of the pollen cell wall by the pistil is a key mechanism by which these 20 teosinte females reject foreign (but closely related) pollen. 21 2 22 One sentence summary: 23The Tcb1-female gene encodes a Pectin Methylesterase that in teosinte silks prevents fertilization 24 by maize pollen. 25 Short title: 26Reproductive isolation and mate rejection in Zea 27 Main text: 28Maize (Zea mays ssp mays) was domesticated from annual teosinte (Zea mays ssp 29 parviglumis) in the Balsas River valley of Mexico (1). In some locations, sympatric populations 30 of domesticated maize and annual teosinte grow in intimate associate and flower synchronously, 31 but rarely produce hybrids (2, 3). In sexually reproducing plants, reproductive barriers exist at 32 different stages, including pre-pollination, post-pollination, and post-fertilization. Post-pollination 33 barriers depend on interaction between the pollen grain and the female reproductive organs (stigma, 34 style, and ovule). In Zea mays, haplotypes at three loci, Gametophyte factor1-s (Ga1-s), 35 Gametophyte factor2-s (Ga2-s), and Teosinte crossing barrier1-s (Tcb1-s), confer Unilateral 36Cross-Incompatibility. While Ga1-s and Ga2-s are widespread in domesticated maize, Tcb1-s is 37 almost exclusively found in wild teosinte populations. The Tcb1-s haplotype, like Ga1-s and Ga2-38 s, confers unilateral cross-incompatibility against varieties carrying the tcb1 (or ga1 or ga2) 39 haplotype. Viewed otherwise, Tcb1-s provides a pollen function that overcomes the crossing 40 barrier. The latter view is preferred since pollen containing both Tcb1-s and tcb1 haplotypes 41fertilizes Tcb1-s plants, indicating that Tcb1-s compatibility is not overcome by the Tcb1-s : tcb1 42 mismatch, as is also the case for the Ga1 and Ga2 systems (4, 5). Tcb1-s was first described in 43 311The authors would like to thank Kathy Barton for the support that kept this project alive, 312 encouragement, and insight and members of the Evans and Barton labs past and present for helpful 313 discussions. We would also like to thank Beverly Oashgar for help with the screen for loss-of-314 function mutants and David Heller, Lance Cabalona, Clayton Coker, Amber Glowacki, and 315Hannah Vahldick for help growing plants and making crosses. We would also like to thank Jeffrey 316Ross-Ibarra for help i...

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“…This type of incompatibility is seen in Solanum 12, 13 , as well as in some Brassica species 14 . It is possible that the action of both the male and female may be conferred through the actions of a protein encoded by a single gene, or the male and female functions of a crossing barrier may be provided by separate genes in tight genetic linkage, as has been shown for tcb1 15 .…”

Section: Introductionmentioning

confidence: 99%

Genome wide analysis ofGa1-smodifiers in maize

Hurst

Liang

Smith

et al. 2019

Preprint

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A one way reproductive barrier exists between most popcorn varieties and dent corn varieties grown in the United States. This barrier is predominantly controlled by the ga1 locus. Using data from a diverse population of popcorn accessions pollinated by a dent corn tester, we found that the non-reciprocal pollination barrier conferred by ga1 is more complex than previously described. Individual accessions ranged from 0% to 100% compatible with dent corn pollen. Using conventional genotyping-by-sequencing data from 371 popcorn accessions carrying Ga1-s, seven significant modifiers of dent pollen compatibility were identified on five chromosomes. One locus may either be a nonfunctional ga1 allele present within popcorn, or second necessary gene for the reproductive barrier in genetic linkage with ga1, while the other modifiers are clearly genetically unlinked. The existence of ga1 modifiers segregating in a popcorn genetic background may indicate selective pressure to allow gene flow between populations, which should be incorporated into future models of the impact of genetic incompatibility loci on gene flow in natural and agricultural plant populations.

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Genetic and cellular analysis of cross-incompatibility in Zea mays

Cited by 32 publications

References 34 publications

Teosinte in Europe – Searching for the Origin of a Novel Weed

Teosinte in Europe – Searching for the Origin of a Novel Weed

A Silk-Expressed Pectin Methylesterase Confers Cross-Incompatibility Between Wild and Domesticated Strains of Zea mays

Genome wide analysis ofGa1-smodifiers in maize

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