Genetic and biochemical characterization of hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus

Abstract

Hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus, is a Bsa variant produced by S. hyicus 3682, a strain isolated from bovine milk. Hyicin 3682 is found in the culture supernatant, is bactericidal and its producing strain exhibits a much broader spectrum of antimicrobial activity than the producing strain of Bsa against several Gram-positive bacteria, which include foodborne pathogens, food-spoilage microorganisms and bacterial species of medical and veterinary importance. Sequencing of the genome of S. hyicus 3682 provided the nucleotide sequence of the entire gene cluster involved in hyicin 3682 production, which seems to be located on pRJ109, the single plasmid carried by this strain. This gene cluster is expressed and consists of 8525bp and of eight genes (hyiA, hyiB, hyiC, hyiD, hyiP, hyiF, hyiE and hyiG) encoded on the same DNA strand. The mature lantibiotic exhibits 91% identity to Bsa and its molecular mass was found to be ∼26Da higher due to two amino acid substitutions. S. hyicus 3682 proved to be only partially immune to its cognate bacteriocin up to 1024 AU/ml. Therefore, hyicin 3682, the first Bsa variant reported in coagulase-negative staphylococci, does exhibit antimicrobial and siblicidal activities.

Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens

“…The Staphylococcus genus has been widely described in the literature as a bacteriocin producer. Several studies have described staphylococcins in strains from food products, such as milk (Hyicin 3682, Aureocin A70, and Aureocin A53) ( Netz et al, 2001 ; Netz et al, 2002 ; Carlin Fagundes et al, 2017 ), fermented food (Micrococcin P1 and Nukacin ISK-1) ( Cundliffe and Thompson, 1981 ; Sashihara et al, 2000 ), fish (Nukacin KQU-131) ( Wilaipun et al, 2008 ), livestock (BacCH91 and Gallidermin) ( Kellner et al, 1988 ; Wladyka et al, 2013 ), and also in strains from pets (BacSp222) ( Wladyka et al, 2015 ). However, very few studies have reported bacteriocins produced by staphylococci from wild animals, except for some studies on animals from the marine environment ( Prichula et al, 2021 ).…”

A Review on Enterocin DD14, the Leaderless Two-Peptide Bacteriocin with Multiple Biological Functions and Unusual Transport Pathway

“…The purification of bacteriocins, including leaderless ones, is usually carried out with a multistep procedure including ammonium sulfate precipitation, followed by one or more chromatography-based methods [66,67]. This strategy was largely utilized to purify bacteriocins devoid of leader peptide [38,68], such as enterocins L50A and L50B [20], A5-11A and A5-11B [37], and 62-6 [69]. Nonetheless, this strategy is time-consuming, expensive, and leads to low yields of purified peptides [67,70].…”

Comprehensive Approaches for the Search and Characterization of Staphylococcins

“…Staphylococcin-producing isolates in food have been widely reported in the literature, especially in milk and fermented food (Hyicin 3682 [ 81 ], Aureocin A70 [ 71 , 72 ], Aureocin A53 [ 74 ], Gallidermin [ 86 , 87 , 88 , 89 , 90 ], Micrococcin P1 [ 85 ], Nukacin ISK-1 [ 95 , 96 , 97 , 98 , 99 ], Nukacin KQU-131 [ 91 ], Warnericin RB4 [ 94 ] and the recently discovered Homicorcin [ 92 ]) ( Table 1 and Table 2 ). Moreover, recent works have reported the presence of bacteriocin-producing CoNS isolates in chicken-derived food that can act as protectors against other contaminant or pathogenic bacteria.…”