Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens

Fernández-Fernández, Rosa; Lozano, Carmen; Eguizábal, Paula; Ruiz-Ripa, Laura; Martínez-Álvarez, Sandra; Abdullahi, Idris Nasir; Zarazaga, Myriam; Torres, Carmén

doi:10.3389/fmicb.2022.870510

Cited by 10 publications

(11 citation statements)

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“…However, studies reporting the frequency of antimicrobial activity in bacteria of wildlife and livestock animals are limitated ( Poeta et al, 2007 ; Almeida et al, 2011 ), and those focused on Staphylococcus are even scarcer ( Fernández-Fernández et al, 2022 ), and storks have never been studied in this respect. The current work represents to the best of our knowledge, the largest study of antimicrobial activity, as well as the presence of bacteriocin structural genes in nasotracheal Staphylococcus isolates recovered from healthy wild storks.…”

Section: Discussionmentioning

confidence: 99%

“…The AP isolates did not show antimicrobial activity against Gram-negative bacteria, such as E. coli and P. aeruginosa . Recent studies have detected Staphylococcus with antimicrobial activity among staphylococci of wild mammals and birds (excluding storks) ( Fernández-Fernández et al, 2022 ), human nares ( Janek et al, 2016 ), and those recovered from food ( Van der Veken et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriocin gene detection. The presence of 22 bacteriocin structural genes was tested by PCR and sequencing in all the AP-isolates detected in this study ( aurA, aucA, epiA, sacaA/sacbA, gdmA, bacSp222, nsj, hyiA, hycS, bacCH91, bsaA2, acIA, ale-1, lss, nukA, nkqA, eciA, pepA, elxA, elkA, ecdA, orf4 ), as well as three bacteriocin gene families described elsewhere (BS, GEST, and NUK) ( Fernández-Fernández et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation

et al. 2023

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A collection of 259 staphylococci of 13 different species [212 coagulase-negative (CoNS) and 47 coagulase-positive (CoPS)] recovered from nasotracheal samples of 87 healthy nestling white storks was tested by the spot-on-lawn method for antimicrobial-activity (AA) against 14 indicator bacteria. Moreover, extracts of AP isolates were obtained [cell-free-supernatants (CFS) both crude and concentrated and butanol extracts] and tested against the 14 indicator bacteria. The microbiota modulation capacity of AP isolates was tested considering: (a) intra-sample AA, against all Gram-positive bacteria recovered in the same stork nasotracheal sample; (b) inter-sample AA against a selection of representative Gram-positive bacteria of the nasotracheal microbiota of all the storks (30 isolates of 29 different species and nine genera). In addition, enzymatic susceptibility test was carried out in selected AP isolates and bacteriocin encoding genes was studied by PCR/sequencing. In this respect, nine isolates (3.5%; seven CoNS and two CoPS) showed AA against at least one indicator bacteria and were considered antimicrobial-producing (AP) isolates. The AP isolates showed AA only for Gram-positive bacteria. Three of these AP isolates (S. hominis X3764, S. sciuri X4000, and S. chromogenes X4620) revealed AA on all extract conditions; other four AP isolates only showed activity in extracts after concentration; the remaining two AP isolates did not show AA in any of extract conditions. As for the microbiota modulation evaluation, three of the nine AP-isolates revealed intra-sample AA. It is to highlight the potent inter-sample AA of the X3764 isolate inhibiting 73% of the 29 representative Gram-positive species of the nasotracheal stork microbiota population. On the other hand, enzymatic analysis carried out in the two highest AP isolates (X3764 and X4000) verified the proteinaceous nature of the antimicrobial compound and PCR analysis revealed the presence of lantibiotic-like encoding genes in the nine AP isolates. In conclusion, these results show that nasotracheal staphylococci of healthy storks, and especially CoNS, produce antimicrobial substances that could be important in the modulations of their nasal microbiota.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation

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“…As for S. hominis, it is a normal skin commensal coagulase negative staphylococci (CoNS) described as a bacteriocin producer such as hominicin [29] and nukacin KQU-131 [30], among others. Moreover, recent studies have detected bacteriocin-like-producing staphylococci of environmental origin, including S. hominis [31]. Due to their high tolerance to an acidic environment, the resistance to bile, and the capacity to adhering to an epithelial cell line, S. hominis has been proposed as a good candidate for probiotic treatments against S. aureus [32].…”

Section: Discussionmentioning

confidence: 99%

Citizen Contribution for Searching for Alternative Antimicrobial Activity Substances in Soil

et al. 2022

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Antimicrobial resistance (AMR) is problematic worldwide, and due to the loss of efficiency of many antibiotics, the pressure to discover alternative antimicrobial molecules has increased. Soil harbors a great biodiversity and biomass of microorganisms, and many antibiotics are produced by soil microbiota. Therefore, soil is a promising reservoir to find new antimicrobial agents. In this respect, novel pedagogical strategies regarding the AMR global crisis have recently been developed in different countries worldwide. Highlighted is the service-learning project “MicroMundo” integrated in a global Citizen Science project called “Tiny Earth”. Hence, the present work aimed at determining the antimicrobial activity of soil bacteria, the biodiversity of the selected isolates as putative antimicrobial producers, and their antibiotic resistance profile. Moreover, through the MicroMundo project, we tried to illustrate the relevant link between science and education and the benefits of implementing service-learning methodologies to raise awareness of the AMR problem and to contribute to the search for new alternatives. A total of 16 teachers, 25 university students and 300 secondary school students participated in the search for antimicrobial activity on a collection of 2600 isolates obtained from a total of 130 soil samples analysed. In total, 132 isolates (5% of total tested) were selected as potential antimicrobial producers when two indicator bacteria were used (Escherichia coli and Staphylococcus epidermidis); the most frequent genus among these isolates was Bacillus, followed by Pseudomonas, Paenibacillus and Serratia. The antimicrobial activity (AA) of the 132 potential antimicrobial producers was studied in a second step against 15 indicator bacteria (of six genera and thirteen species, including relevant pathogens). Of the 132 potentially producing bacteria, 32 were selected for further characterization. In this respect, 18 isolates showed low AA, 12 isolates were considered as medium producers, and 2 highly antimicrobial-producing isolates were found (Brevibacillus laterosporus X7262 and Staphylococcus hominis X7276) showing AA against 80% of the 15 indicators tested. Moreover, 48% of the antimicrobial-producing bacteria were susceptible to all antibiotics tested. Due to citizen science, antimicrobial-producing bacteria of great interest have been isolated, managing to raise awareness about the problem of AMR.

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“…Moreover, the presence of the lug D gene was taken as a reference to identify the genetic cluster associated with the production of the non-ribosomal peptide (NRP), lugdunin (GenBank accession number NC_017353.1). For that, the lug D amplicon (189 pb) was identified using the following primers and PCR conditions: F-TTCGGGAACTACTGGAATGC (Tm = 60.1 °C), R-AAATGCAATGTCCCTCCAAC (Tm = 59.8 °C); 1 cycle at 94 °C for 7 min, 30 cycles at 94 °C for 1 min, 57 °C for 1 min, 72 °C for 1 min, and finally 72 °C for 10 min [ 15 ]. Subsequently, lug D amplicons were confirmed by Sanger sequencing.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals

et al. 2022

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Staphylococcus lugdunensis is a coagulase-negative-staphylococci (CoNS) that lately has gained special attention in public health as a human pathogen and also as a bacteriocin-producer bacteria. In this study, we characterized 56 S. lugdunensis isolates recovered from human samples in two Spanish hospitals. Antimicrobial susceptibility testing was performed and antimicrobial resistance and virulence genotypes were determined. Antimicrobial activity (AA) production was evaluated by the spot-on-lawn method against 37 indicator bacteria, including multidrug-resistant (MDR) isolates, and the presence of the lugD gene coding for lugdunin bacteriocin was analyzed by PCR. The antibiotic resistance detected was as follows (% resistance/genes detected): penicillin (44.6%/blaZ), oxacillin (1.8%/mecA on SCCmec-V), erythromycin-clindamycin inducible (7.1%/erm(C), msrA), tetracycline (5.3%/tetK), gentamicin and/or tobramycin (3.6%/ant(4′)-Ia, acc(6′)-aph(2″)), and fosfomycin (21.4%). A MDR phenotype was detected in 5% of isolates. Twenty-one of the S. lugdunensis isolates showed susceptibility to all 20 antibiotics tested (37.5%). The screening for AA revealed 23 antimicrobial producer (AP) isolates with relevant inhibition against coagulase-positive-staphylococci (CoPS), including both methicillin-susceptible and –resistant S. aureus. The lugD gene was detected in 84% of the 56 S. lugdunensis isolates. All of the AP S. lugdunensis isolates (n = 23) carried the lugD gene and it was also detected in 24 of the non-AP isolates, suggesting different gene expression levels. One of the AP isolates stood out due to its high antimicrobial activity against more than 70% of the indicator bacteria tested, so it will be further characterized at genomic and proteomic level.

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Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens

Cited by 10 publications

References 49 publications

Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation

Detection of antimicrobial producing Staphylococcus from migratory birds: Potential role in nasotracheal microbiota modulation

Citizen Contribution for Searching for Alternative Antimicrobial Activity Substances in Soil

Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals

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