Genetic and biochemical analysis of a laboratory‐selected spirodiclofen‐resistant strain of <i>Tetranychus urticae</i> Koch (Acari: Tetranychidae)

Pottelberge, Steven Van; Leeuwen, Thomas Van; Khajehali, Jahangir; Tirry, Luc

doi:10.1002/ps.1698

Cited by 120 publications

(141 citation statements)

References 30 publications

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“…Additionally, the selection provided only moderate to low cross-resistance to spiromesifen in larvae and eggs (RR=30.4 and 4, respectively), as well as low cross-resistance to spirotetramat in larvae (RR=3). Agespecific expression of resistance to spirodiclofen in larvae and eggs (RR=274 and 2, respectively) and moderate level of cross-resistance between this acaricide and spiromesifen (RR=18, larvae), was previously shown in SR-VP strain, a laboratory-selected resistant strain of T. urticae (van Pottelberge et al, 2009b). Spirodiclofen resistance in another strain selected in laboratory, SR-FZ strain of P. citri, was age-specific as well (RR=51.2-157, juvenile stages; RR=4.6, eggs), and the strain was moderate crossresistant to spirotetramat (Yu et al, 2011).…”

Section: Resistance and Cross-resistancementioning

confidence: 69%

“…Spirodiclofen resistance in another strain selected in laboratory, SR-FZ strain of P. citri, was age-specific as well (RR=51.2-157, juvenile stages; RR=4.6, eggs), and the strain was moderate crossresistant to spirotetramat (Yu et al, 2011). In these two strains, the fertility of female adults was not affected after the treatment with spirodiclofen, in contrast to that of females from corresponding susceptible strains (van Pottelberge et al, 2009a, Yu et al, 2011.…”

Section: Resistance and Cross-resistancementioning

confidence: 93%

“…Spirodiclofen and spiromesifen showed high activity against various T. urticae and P. ulmi strains resistant to dicofol, organophosphates, mite growth inhibitors, abamectin, METI acaricides, Nauen and Konanz, 2005;Pree et al, 2005;van Pottelberge et al, 2009b). Similarly, none of B. tabaci strains resistant to endosulfan, organophosphates, carbamates, pyrethroids, neonicotinoids, pyriproxyfen, diafenthiuron, showed any cross-resistance to spiromesifen (Nauen and Konanz, 2005;Prabhaker et al, 2008;Kontsedalov et al, 2009).…”

Section: Resistance and Cross-resistancementioning

confidence: 99%

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Cyclic ketoenols: Acaricides and insecticides with a novel mode of action

Marčić

Perić

Petronijevic

et al. 2011

Pesticidi i fitomedicina

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SUMMARYModern crop protection industry puts great efforts into the investigation and development of pesticides (insecticides, acaricides) with a novel mode of action, primarily because of increasingly important problem of resistance of pest organisms to pesticides. Resistance of many pest arthropod species (insects and mites) to insecticides and/or acaricides became a global phenomenon in the last decades. Therefore, pest management heavily relies on constant introduction of novel active ingredients in use, i.e. on the alternative use of a number of compounds with different modes of action in order to save the longevity of newly developed products. Among pest arthropods, according to the potential for resistance development and a number of documented cases, spider mites (Acari: Tetranychidae), especially species Tetranychus urticae and Panonychus ulmi, as well as certain species of whiteflies and leaf aphids (Homoptra: Aleyrodidae, Aphididae) stand out.In 2002, the company Bayer CropScience introduced spirodiclofen, a tetronic acid derivative and the first cyclic ketoenol, acaricide with a wide spectrum and a novel mode of action -inhibition of acetyl-CoA-carboxylase. Soon after, spiromesifen was introduced, also a derivative of tetronic acid, acaricide and insecticide intended for control of phytophagus mites and whiteflies. Spirotetramat, a tetramic acid derivative, and the third member of the ketoenol group, was recently commercialised as an insecticide efficient against whiteflies, leaf aphids and other harmful Homoptera. In this paper are presented the most significant properties of cyclic ketoenols, aiming at pointing to the possibilities and prospects of their use, but also to potential problems and limitations.

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Section: Resistance and Cross-resistancementioning

confidence: 69%

Section: Resistance and Cross-resistancementioning

confidence: 93%

Section: Resistance and Cross-resistancementioning

confidence: 99%

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Cyclic ketoenols: Acaricides and insecticides with a novel mode of action

Marčić

Perić

Petronijevic

et al. 2011

Pesticidi i fitomedicina

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“…Rauch&Nauen (2003) ve Van Pottelberge et al (2009a), T. urticae'de seleksiyon baskısı sonucu spirodiclofene direnç geliştiğini belirtmişlerdir. Van Pottelberge et al (2009b), spirodiclofenin iki noktalı kırmızıörümceklerde larva döneminde öldürücü etki yaptığını, ergin dönemde işe dişilerde bırakılan yumurta sayısını azalttığını bildirmişlerdir. Ferreira et al (2015), gül ve krizantem üzerinden topladıkları abamectin dirençli T. urticae popülasyonlarında spirodiclofen karşı 390 ve 400 kat çapraz direnç bulmuşlardır.…”

Section: Araştırma Sonuçları Ve Tartışma Bioassay Test Sonuçlarıunclassified

“…Rauch & Nauen (2003) gül bitkisi üzerinden toplanan spirodiclofen dirençli T. urticae'de spirodiclofen direnç gelişiminde esteraz enziminin rol oynadığını bildirmişlerdir. Pottelberge et al (2009) 274 kat spirodiclofen dirençli T. urticae popülasyonunda esteraz ve enziminin direnç gelişiminde rol oynadığını belirlemişlerdir. Çalışmamızda da T. urticae'nin karanfil seralarından toplanan tarla popülasyonlarında abamectin ve spirodiclofen akarisitlerine karşı gelişen dirençte esteraz enziminin büyük öneme sahip olduğu düşünülmektedir.…”

Section: Esteraz Enzimi Kinetik Okuma Sonuçlarıunclassified

Tetranychus urticae Koch (Acari:Tetranychidae)'nin karanfil popülasyonlarında abamectin ve spirodiclofen'e karşı duyarlılık düzeyleri

Salman¹,

Kocaman²

2017

Türk. entomol. bült.

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SummaryTetranychus urticae is a harmful species which causes important economic losses in cut flowers. Sensitivity levels against abamectin and spirodiclofen has been determined by using petri plate-spray tower method in four T. urticae populations which was collected from clove greenhouses. It was determined that there was 43.53-246.23 fold resistance against abamectin and 30.49-118.78 fold resistance against spirodiclofen in two spotted spider mite populations. Also, polyacrylamide gel electrophoresis and kinetic reading methods have been applied for the purpose of determining the relationship of resistance development with esterase enzyme. The thickest esterase band of two spotted spider mite populations in electrophoresis gels has been determined in K3 population. Also, 4.48 fold esterase enzyme activities has been found in the same population as the result of kinetic reading of esterase enzyme. These findings indicate that esterase enzyme could be effective in abamectin and spirodiclofen resistance development in clove populations of T. urticae. Keywords: Tetranychus urticae, sensitivity, acaricide, esterase ÖzetTetranychus urticae kesme çiçeklerde önemli ekonomik kayıplara neden olan zararlı bir türdür. Karanfil seralarından toplanan dört adet T. urticae popülasyonlarındaabamectin ve spirodiclofen'e karşı duyarlılık düzeyleri petri kabı-ilaçlama kulesi yöntemi kullanılarak belirlenmiştir. İki noktalı kırmızıörümcek popülasyonlarında abamectine karşı 43.53-246.23 kat ve spirodiclofen'e karşı 30.49-118.78 kat direnç belirlenmiştir. Ayrıca direnç gelişiminin esteraz enzimi ile olan ilişkisini belirlemek amacıyla poliakrilamid jel elektroforez ve kinetik okuma yöntemleri uygulanmıştır. İki noktalı kırmızıörümcek popülasyonlarının elektroforez jellerinde en kalın esterazbantı K3 popülasyonunda belirlenmiştir. Ayrıca esteraz enziminin kinetik okuması sonucunda aynı popülasyonda 4.48 kat esteraz enzim aktivitesi bulunmuştur. Bu bulgular T. urticae'nin karanfil popülasyonlarında abamectin ve spirodiclofen direnç gelişiminde esteraz enziminin etkisi olabileceğini göstermektedir.

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MOLECULAR CHARACTERIZATION OF TWO CARBOXYLESTERASE GENES OF THE CITRUS RED MITE, Panonychus citri (ACARI: TETRANYCHIDAE)

Zhang

Niu

Ding

et al. 2013

Arch Insect Biochem Physiol

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The citrus red mite, Panonychus citri, is known for its ability to rapidly evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. To get better insight into the detoxification mechanism of P. citri, two carboxylesterase (CarE) genes, PCE1 and PCE2, were isolated and characterized. PCE1 and PCE2 contained open reading frames of 1,653 and 1,392 nucleotides, encoding proteins of 550 and 463 amino acid residues, respectively. Phylogenetic analyses showed that PCE1 and PCE2 were most closely related to the CarE genes from other phytophagous mites. The transcriptional profiles of two CarE genes among developmental stages (egg, larva, nymph, adult female, and adult male), after exposing to four acaricides (avermectin, azocyclotin, pyridaben, and spirodiclofen) and acid rain were investigated using real-time quantitative PCR (qPCR). The results showed that during development, PCE1 was highly expressed at the egg stage, whereas PCE2 was abundantly expressed at the adult stage of males. The expression levels of PCE1 were highly induced upon exposure to acaricides and acid rain. On the other hand, the expression levels of PCE2 were increased after treatment with avermectin and pyridaben. These results suggest that PCE1 and PCE2 may have distinct roles in different developmental stages and participate in the detoxification of acaricides.

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Genetic and biochemical analysis of a laboratory‐selected spirodiclofen‐resistant strain of Tetranychus urticae Koch (Acari: Tetranychidae)

Cited by 120 publications

References 30 publications

Cyclic ketoenols: Acaricides and insecticides with a novel mode of action

Cyclic ketoenols: Acaricides and insecticides with a novel mode of action

Tetranychus urticae Koch (Acari:Tetranychidae)'nin karanfil popülasyonlarında abamectin ve spirodiclofen'e karşı duyarlılık düzeyleri

MOLECULAR CHARACTERIZATION OF TWO CARBOXYLESTERASE GENES OF THE CITRUS RED MITE, Panonychus citri (ACARI: TETRANYCHIDAE)

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