Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing

Volkov, A. A.; Volkov, I. A.; Plugov, Aleksandr G.; Kulyabina, E. V.; Melkova, Olga N.; Lavrov, Georgii S.; Bocharova, D. V.; Alekseev, Ya. I.

doi:10.32446/0368-1025it.2021-1-60-65

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Sequencing was performed with the same primers on a Nanophore 05 [35] sequencer with a BigDye™ TerminatorKit v.1.1/3.1 (Applied Biosystems, Waltham, MA, USA). Further analysis and alignment of the obtained sequences was carried out with the Geneious bioinformatics software package.…”

Section: Methodsmentioning

confidence: 99%

Biological Characteristics of a Rare and Vulnerable Species (SCHIZOTHORAX ARGENTATUS (Kessler, 1874)) of TOKYRAUYN RIVER and Approbation of Its Artificial Reproduction

et al. 2022

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As a result of the complex impact of anthropogenic factors on the ecosystem of Balkhash Lake, the main commercial species of fish—endemic—Balkhash marinka was on the verge of extinction. Artificial reproduction is becoming increasingly important every year in the complex of works to maintain the commercial stocks of valuable fish species as well as to preserve rare and endangered populations. Despite the fact that attempts at artificial reproduction have been repeatedly made, to date no scientific and methodological basis has been created to study and preserve rare and endangered fish species in the Republic of Kazakhstan and there are no adapted technologies for the formation of repair herds of fish of this category for the fish breeding enterprises of the reproductive complex of Kazakhstan. The presence of a replacement stock of the Balkhash marinka will further contribute to the development and improvement of biotechnical methods for their breeding and cultivation of viable stocking material. The stocking of natural water bodies with viable fish seeds and the maintenance of living collections of rare and endangered fish species will contribute to their conservation in natural conditions and, as a reserve, in the country’s fish farms. The results of the development of effective technologies for artificial reproduction and the formation of a replacement stock of the Balkhash marinka in industrial conditions contribute to solving the fundamental problem associated with the preservation of rare and endemic fish species from complete extinction, genetic diversity and the rational use of the potential of natural populations. An analysis of genetic identification showed that the breeders of marinka, according to the results of sequencing the barcoding gene CO1 of mitochondrial DNA, belong to the species Balkhash marinka S. argentatus with an accuracy of 99–100%.

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Section: Methodsmentioning

confidence: 99%

Biological Characteristics of a Rare and Vulnerable Species (SCHIZOTHORAX ARGENTATUS (Kessler, 1874)) of TOKYRAUYN RIVER and Approbation of Its Artificial Reproduction

et al. 2022

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“…For example, in the human genome of six billion base pairs in size, more than 25000 genes have been found, which contain more than three million single nucleotide substitutions, determining the phenotypic diversity of the species Homo sapiens [5]. The replacement of only one nucleotide can change the function of an organism under study, therefore, the accuracy of DNA sequencing is one of the essential parameters that require careful evaluation [6]. Historically, Sanger's massive genome sequencing began with the introduction of automated DNA sequencers.…”

mentioning

confidence: 99%

Determination of the Maximum Length of DNA in a Polymer Based on Linear Poly(N,N-Dimethylacrylamide) Decoded with an Accuracy of 99% by Capillary Gel Electrophoresis with Laser-Induced Fluorescence

et al. 2021

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DNA sequencing is an essential task of modern molecular genetic research. Its fundamental parameter is the accuracy of decoding. In this work, we determined the maximum lengths of DNA fragments, obtained by the Sanger method and sequenced with an accuracy of 99%, in a polymer based on linear poly(N,N-dimethylacrylamide) PDMA-6 for capillaries 50 cm long to the optical window of the detector of a Nanophore 05 domestic genetic analyzer and an imported GA 3500xL genetic analyzer. The results of DNA sequencing obtained by various analysis algorithms were compared. The parameters of the analysis algorithms were selected, ensuring best results of DNA sequencing in using the PDMA-6 polymer.

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Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing

Cited by 2 publications

References 12 publications

Biological Characteristics of a Rare and Vulnerable Species (SCHIZOTHORAX ARGENTATUS (Kessler, 1874)) of TOKYRAUYN RIVER and Approbation of Its Artificial Reproduction

Biological Characteristics of a Rare and Vulnerable Species (SCHIZOTHORAX ARGENTATUS (Kessler, 1874)) of TOKYRAUYN RIVER and Approbation of Its Artificial Reproduction

Determination of the Maximum Length of DNA in a Polymer Based on Linear Poly(N,N-Dimethylacrylamide) Decoded with an Accuracy of 99% by Capillary Gel Electrophoresis with Laser-Induced Fluorescence

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