Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number

Kubo, Fumika Clara; Yasui, Yukihiko; Kumamaru, Toshihiro; Satō, Yutaka; Hirano, Hideki

doi:10.1266/ggs.16-00018

Cited by 10 publications

(3 citation statements)

References 24 publications

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“…1a). About six NARROW LEAF (NAL) genes controlling leaf width have been reported in rice [30][31][32][33][34][35] , and among them, NAL1 was shown to have the strongest effect on leaf width. We identi ed 13 NAL genes in the three Saccharum genomes (Fig.…”

Section: Genes Related To the Key Characteristics Of Saccharummentioning

confidence: 99%

Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum

Zhang

Pan

et al. 2022

Nat Genet

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S. spontaneum is a founding Saccharum species that contributes stress resistance to the genetic background of modern sugarcane cultivars. Here, we have assembled the autopolyploid S. spontaneum Np-X genome with ancestral form into 40 pseudo-chromosomes in 10 homologous groups, revealing the recent chromosome reduction and polyploidization that occurred in Saccharum. The paleo-duplicated chromosomal pairs exhibit functional redundancy in Saccharum and underwent ssion followed by fusion accompanied by centromeric spreading around 0.80 million years ago (Mya) before evolving into their current forms with basic chromosome numbers x = 9 and x = 8 in S. spontaneum, likely in a stepwise manner. WGDs occurred independently in Saccharum species around 1.5 Mya. Highly diverse chromatin structures exist among homologous chromosomes despite their high collinearity, and the re-structuring of NpChr5 and NpChr8 might have suppressed switching of chromatin structure from inactive to active. Resequencing of 116 sugarcane accessions elucidated that the S. spontaneum originated from North India and that the basic chromosome numbers x = 8, x = 9, and x = 10 originated independently, indicating that recent chromosome reduction rather than polyploidization has driven the adaptive evolution of Saccharum. Our study provides genomic resources and suggests new directions for accelerating sugarcane improvement and advances our knowledge of the evolution of auto-polyploids.

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Section: Genes Related To the Key Characteristics Of Saccharummentioning

confidence: 99%

Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum

Zhang

Pan

et al. 2022

Nat Genet

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“…For example, OsEXPA8-OX plants showed more cells in many small veins [61]. Vascular bundles act as channels for water and nutrients and for mechanical support in plants [68]. An increase in the number of large veins with bigger and more numerous cells would contribute to enhanced growth in OsEXPA7-OX plants.…”

Section: Osexpa7 Overexpression Increased Cell Size and Enhanced Planmentioning

confidence: 99%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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Expansins are key regulators of cell-wall extension and are also involved in the abiotic stress response. In this study, we evaluated the function of OsEXPA7 involved in salt stress tolerance. Phenotypic analysis showed that OsEXPA7 overexpression remarkably enhanced tolerance to salt stress. OsEXPA7 was highly expressed in the shoot apical meristem, root, and the leaf sheath. Promoter activity of OsEXPA7:GUS was mainly observed in vascular tissues of roots and leaves. Morphological analysis revealed structural alterations in the root and leaf vasculature of OsEXPA7 overexpressing (OX) lines. OsEXPA7 overexpression resulted in decreased sodium ion (Na+) and accumulated potassium ion (K+) in the leaves and roots. Under salt stress, higher antioxidant activity was also observed in the OsEXPA7-OX lines, as indicated by lower reactive oxygen species (ROS) accumulation and increased antioxidant activity, when compared with the wild-type (WT) plants. In addition, transcriptional analysis using RNA-seq and RT-PCR revealed that genes involved in cation exchange, auxin signaling, cell-wall modification, and transcription were differentially expressed between the OX and WT lines. Notably, salt overly sensitive 1, which is a sodium transporter, was highly upregulated in the OX lines. These results suggest that OsEXPA7 plays an important role in increasing salt stress tolerance by coordinating sodium transport, ROS scavenging, and cell-wall loosening.

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“…In contrast, slight defects in abaxial identity produce rolled leaves, in which other characteristics are largely normal (Yan et al, 2008;Zhang et al, 2009). Mutations in genes for auxin synthesis and for some transcription factors cause a narrow leaf phenotype, together with a reduction in the number of vascular bundles (Fujino et al, 2008;Cho et al, 2013;Ishiwata et al, 2013;Kubo et al, 2016). To understand the genetic mechanisms underlying rice leaf morphogenesis in more detail, we focused on the half-pipe-like leaf1 (hal1) mutant, which has curled leaves, in this paper.…”

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confidence: 99%

Characterization of a <i>half-pipe-like leaf1</i> mutant that exhibits a curled leaf phenotype

et al. 2017

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Leaf forms are diverse in angiosperms, and different types of cells are differentiated depending on the species. Rice leaves are composed of a leaf blade, a leaf sheath and the junction region between them. Cells with characteristic features, such as bulliform cells and sclerenchyma cells, are differentiated in the leaf blade, together with standard epidermal and mesophyll cells. To understand the genetic mechanism underlying leaf morphogenesis in rice, we focused on a mutant, half-pipe-like leaf1 (hal1), whose leaves are adaxially curled. Histological observation revealed that the bulliform cells, which are responsible for leaf rolling under dry conditions, were small in size and abnormal in shape in a semidominant hal1-d mutant. Bulliform cell files were often ambiguous in semi-transparent hal1-d leaves cleared by the TOMEI method, suggesting that the bulliform cells were undeveloped. Therefore, a reduction in the growth of the bulliform cells seemed to be a major cause of leaf curling in the hal1-d mutant. The hal1-d mutation also affected the size of the leaf blade and the spikelet.

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Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number

Cited by 10 publications

References 24 publications

Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum

Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Characterization of a <i>half-pipe-like leaf1</i> mutant that exhibits a curled leaf phenotype

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