Genetic analysis of resistance to root‐lesion nematode (<i>Pratylenchus thornei</i>) in wheat

Zwart, Rebecca S.; Thompson, John; Godwin, Ian D.

doi:10.1111/j.1439-0523.2004.00986.x

Cited by 33 publications

(31 citation statements)

References 13 publications

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“…Classical examples of traits that are difficult and laborious to measure are cereal cyst nematode and root lesion nematode resistance in wheat (Eastwood et al 1991;Eagles et al 2001;Zwart et al 2004 These advantages can be exploited by breeders to accelerate the breeding process (Ribaut & Hoisington 1998;Morris et al 2003). Target genotypes can be more effectively selected, which may enable certain traits to be 'fast-tracked', resulting in quicker line development and variety release.…”

Section: Overview Of Dna Markers Qtl Mapping and Marker-assisted Sementioning

confidence: 99%

Marker-assisted selection: an approach for precision plant breeding in the twenty-first century

Collard

Mackill

2007

Phil. Trans. R. Soc. B

1,703

1,001

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DNA markers have enormous potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection (MAS). The large number of quantitative trait loci (QTLs) mapping studies for diverse crops species have provided an abundance of DNA marker-trait associations. In this review, we present an overview of the advantages of MAS and its most widely used applications in plant breeding, providing examples from cereal crops. We also consider reasons why MAS has had only a small impact on plant breeding so far and suggest ways in which the potential of MAS can be realized. Finally, we discuss reasons why the greater adoption of MAS in the future is inevitable, although the extent of its use will depend on available resources, especially for orphan crops, and may be delayed in less-developed countries. Achieving a substantial impact on crop improvement by MAS represents the great challenge for agricultural scientists in the next few decades.

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Section: Overview Of Dna Markers Qtl Mapping and Marker-assisted Sementioning

confidence: 99%

Marker-assisted selection: an approach for precision plant breeding in the twenty-first century

Collard

Mackill

2007

Phil. Trans. R. Soc. B

1,703

1,001

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“…All experiments were grown in Latinised row-column designs. Nematode experiments were evaluated following the procedure of Thompson et al (1999) with modifications as described by Zwart et al (2004a).…”

Section: Root-lesion Nematode Screeningmentioning

confidence: 99%

“…The genetic basis for resistance to P. thornei in synthetic hexaploid wheat lines has been shown to be polygenic and additive (Zwart et al 2004a). However, to date, only limited sources of resistance to root-lesion nematodes in wheat have been investigated at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Zwart

Thompson²,

Godwin

2005

Aust. J. Agric. Res.

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Abstract. Pratylenchus thornei and P. neglectus are two species of root-lesion nematode that cause substantial yield losses in wheat. No commercially available wheat variety has resistance to both species. A doubled-haploid population developed from a cross between the synthetic hexaploid wheat line CPI133872 and the bread wheat Janz was used to locate and tag quantitative trait loci (QTLs) associated with resistance to both P. thornei and P. neglectus. Wheat plants were inoculated with both species of nematode in independent replicated glasshouse trials repeated over 2 years. Known locations of wheat microsatellite markers were used to construct a framework map. After an initial single-marker analysis to detect marker-trait linkages, chromosome regions associated with putative QTLs were targetted with microsatellite markers to increase map density in the chromosome regions of interest. In total, 148 wheat microsatellite markers and 21 amplified fragment length polymorphism markers were mapped. The codominant microsatellite marker Xbarc183 on the distal end of chromosome 6DS was allelic for resistance to both P. thornei and P. neglectus. The QTL were designated QRlnt.lrc-6D.1 and QRlnn.lrc-6D.1, for the 2 traits, respectively. The allele inherited from CPI133872 explained 22.0-24.2% of the phenotypic variation for P. thornei resistance, and the allele inherited from Janz accounted for 11.3-14.0% of the phenotypic variation for P. neglectus resistance. Composite interval mapping identified markers that flank a second major QTL on chromosome 6DL (QRlnt.lrc-6D.2) that explained 8.3-13.4% of the phenotypic variation for P. thornei resistance. An additional major QTL associated with P. neglectus resistance was detected on chromosome 4DS (QRlnn.lrc-4D.1) and explained a further 10.3-15.4% of the phenotypic variation. The identification and tagging of nematode resistance genes with molecular markers will allow appropriate allele combinations to be selected, which will aid the successful breeding of wheat with dual nematode resistance.Additional keywords: synthetic hexaploid wheat, doubled haploids, composite interval mapping. IntroductionTwo species of root-lesion nematode, Pratylenchus thornei Sher and Allen and P. neglectus (Rensch) Filipjev and Schuurmans Stekhoven, cause substantial yield losses in cereal crops throughout the world. High populations of P. thornei cause up to 85% yield loss in wheat (Triticum aestivum L.) varieties (Thompson et al. 1993). Similarly, P. neglectus causes up to 27% yield loss in wheat varieties (Taylor et al. 1999). In Australia, annual lost wheat production caused by P. thornei and P. neglectus is estimated at $36 million and $12 million, respectively (Brennan and Murray 1998).Glasshouse screening of wheat lines inoculated with pure cultures of nematodes has suggested that the genetic mechanisms that confer resistance to P. thornei do not confer resistance to P. neglectus (Farsi et al. 1995;Taylor et al.

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“…In the first experiment, conducted in 1997, 3 replicates of a subset of 91 RILs were assessed for resistance to P. thornei using the procedure described by Thompson et al (1999). In the second experiment, conducted in 2001, 4 replicates of 129 RILs were assessed for P. thornei resistance based on nematode counts in soil and roots as described by Zwart et al (2004a). Count data were transformed by ln(x + c), where x is nematodes/kg soil and roots sampled, and c is constant.…”

Section: Phenotypic Assessment Of P Thornei Resistancementioning

confidence: 99%

“…durum (Desf.) Husn (2n = 28, AABB) (J. P. Thompson and M. I. Haak, unpublished data), and in synthetic hexaploid wheats produced from their hybridisation (T. aestivum, 2n = 42, AABBDD) (Zwart et al 2004a).…”

Section: Introductionmentioning

confidence: 99%

Mapping quantitative trait loci for resistance to Pratylenchus thornei from synthetic hexaploid wheat in the International Triticeae Mapping Initiative (ITMI) population

Zwart¹,

Thompson²,

Sheedy³

et al. 2006

Aust. J. Agric. Res.

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Abstract. Root-lesion nematode (Pratylenchus thornei) is a serious pathogen of wheat in many countries. The International Triticeae Mapping Initiative (ITMI) population of recombinant inbred lines (RILs) was assessed for resistance to P. thornei to determine the chromosome locations of the resistance genes. The ITMI population is derived from a cross between the resistant synthetic hexaploid wheat W-7984 and a susceptible bread wheat cultivar Opata 85. Two years of phenotypic data for resistance to P. thornei were obtained in replicated glasshouse trials. Quantitative trait locus (QTL) analysis was performed using available segregation and map data for 114 RILs. A QTL on chromosome 6DS showed consistent effects for reduced nematode numbers (partial resistance) across years and accounted for 11% and 23% of the phenotypic variation. A second QTL for P. thornei resistance on chromosome 2BS accounted for an additional 19% and 5%. Restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) markers associated with the QTLs are physically located in regions rich in major genes at the distal ends of the short chromosome arms of 6D and 2B. SSR markers with potential for marker-assisted selection of P. thornei resistance effective in different genetic backgrounds have been identified.

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Genetic analysis of resistance to root‐lesion nematode (Pratylenchus thornei) in wheat

Cited by 33 publications

References 13 publications

Marker-assisted selection: an approach for precision plant breeding in the twenty-first century

Marker-assisted selection: an approach for precision plant breeding in the twenty-first century

Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat

Mapping quantitative trait loci for resistance to Pratylenchus thornei from synthetic hexaploid wheat in the International Triticeae Mapping Initiative (ITMI) population

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