Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers

Abstract

Nine microsatellite loci for genetic analysis of three populations of the tropical tree Eugenia uniflora L. (pitanga or Brazilian cherry) from fragments of semideciduous forest were developed. We used the technique of building a (GA) n and (CA) n microsatellite-enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 84 mature trees found in three areas (Ribeirão Preto, Tambaú and São José do Rio Pardo), highly impacted by the agricultural practices, in a large region among Pardo river and Mogi-Guaçu river basins, in state of São Paulo, Brazil. All loci were polymorphic, and the number of alleles was high, ranging from 6 to 24, with a mean of 14.4. All stands showed the same high level of genetic diversity (mean H E = 0.83) and a low genetic differentiation (mean F ST = 0.031), indicating that genetic diversity was higher within rather than among populations. Seven of the nine loci were highly variable, and sufficiently informative for E. uniflora. It was concluded that these new SSR markers can be efficiently used for gene flow studies.

Fragmentation effects and genetic diversity of the key semidecidual forest species Metrodorea nigra in Southwestern Brazil

“…The average number of alleles per SSR locus found in this study (14.85) was similar to those observed in other tropical tree species such as Eugenia uniflora (14.4;Ferreira-Ramos et al, 2008) and Euterpe edulis (14.7; Conte et al, 2006). The index of genetic diversity in M. nigra (H E = 0.771) was consistent with those observed in other Brazilian forest species, including Cariniana legalis (0.721; Leal et al, 2014), E. edulis (0.781; Conte et al, 2006.…”

“…The index of genetic diversity in M. nigra (H E = 0.771) was consistent with those observed in other Brazilian forest species, including Cariniana legalis (0.721; Leal et al, 2014), E. edulis (0.781; Conte et al, 2006. ), E. uniflora (0.83;Ferreira-Ramos et al, 2008), and Manilkara huberi (0.86; Azevedo et al, 2007).…”

Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers

“…The mean value for H E in M. nigra is comparable to Psidium guajava (H E = 0.62; Risterucci et al, 2005), but lower than other tropical trees: Cariniana estrellensis (H E = 0.73; Guidugli et al, 2009) and Eugenia uniflora (H E = 0.83; Ferreira-Ramos et al, 2008). No evidence of linkage disequilibrium was found between any locus pairs, indicating physical independence of the polymorphic loci.…”

Progress in Myrtaceae genetics and genomics: Eucalyptus as the pivotal genus

“…A resource of 366 microsatellite markers has been published for Eucalyptus (Brondani et al 1998(Brondani et al , 2006Byrne et al 1996;Ottewell et al 2005;Steane et al 2001;Thamarus et al 2002) and a set of 28 for the related genus Corymbia (Jones et al 2001;Shepherd et al 2006). In the non-eucalypt Myrtaceae, 113 microsatellite markers have been developed from Melaleuca (Miwa et al 2000;Rossetto et al 1999a), 24 for Metrosideros Kaneko et al 2007), nine for Eugenia (Ferreira-Ramos et al 2008), 14 for Myrtus (Albaladejo et al 2010), ten for Calothamnus (Elliott and Byrne 2005), eight for Syzygium (Hillyer et al 2007), and 13 for Acca .…”