Abstract:The analysis of 12 X-STR loci included in the Investigator® Argus X-12 kit was performed on a sample of 325 unrelated persons from Vojvodina Province, Republic of Serbia. No significant differences were observed in the allele frequencies in males and females. Heterozygosity values among the studied loci ranged from 67.62 to 94.28%. All loci in female individuals were consistent with the Hardy-Weinberg equilibrium test. The combined power of discrimination values in male and female individuals was 0.9999999994 … Show more
“…Short tandem repeats (STRs) or microsatellites, are present in noncoding intron regions of the human genome and are extensively used for forensic identifications in forensic DNA laboratories all over the world [12][13][14][15][16][17]. STRs usually comprise of tandem repeat motif from 2-6 base pairs.…”
Tibetans are considered an East Asian ethnic group and primarily live in the high Tibetan plateau, the western Sichuan and Yunnan mountains of central and southern China, and areas throughout the Himalayas and around the Tibetan plateau. These people exhibit rare molecular machinery that allows them to adapt to hypoxic environments in the Qinghai-Tibet Plateau and make them a potential candidate for providing insights related to medical genetic, molecular medicine and human population studies. In the current study, we have genotyped 549 individuals with Investigator Argus X-12 Kit. For 12 X-STRs, a total of 174 unique alleles were found, among them DXS10134 and DXS10135 were the most polymorphic loci. All of the loci were in Hardy-Weinberg Equilibrium (HWE). The numbers of observed haplotypes in Highlander Tibetans males were 161,112, 96 and 108, respectively, whereas haplotype diversities (HD) were 0.9959, 0.9880, 0.9809 and 0.9873, respectively. The combined discrimination power for males (PDm) was 0.999 999 99701 and for females (PDf) was 0.999 999 999 999 9958. This study represents an extensive report on X chromosomal STR markers variation in the Highlander Tibetans population for forensic applications and population genetic studies.
“…Short tandem repeats (STRs) or microsatellites, are present in noncoding intron regions of the human genome and are extensively used for forensic identifications in forensic DNA laboratories all over the world [12][13][14][15][16][17]. STRs usually comprise of tandem repeat motif from 2-6 base pairs.…”
Tibetans are considered an East Asian ethnic group and primarily live in the high Tibetan plateau, the western Sichuan and Yunnan mountains of central and southern China, and areas throughout the Himalayas and around the Tibetan plateau. These people exhibit rare molecular machinery that allows them to adapt to hypoxic environments in the Qinghai-Tibet Plateau and make them a potential candidate for providing insights related to medical genetic, molecular medicine and human population studies. In the current study, we have genotyped 549 individuals with Investigator Argus X-12 Kit. For 12 X-STRs, a total of 174 unique alleles were found, among them DXS10134 and DXS10135 were the most polymorphic loci. All of the loci were in Hardy-Weinberg Equilibrium (HWE). The numbers of observed haplotypes in Highlander Tibetans males were 161,112, 96 and 108, respectively, whereas haplotype diversities (HD) were 0.9959, 0.9880, 0.9809 and 0.9873, respectively. The combined discrimination power for males (PDm) was 0.999 999 99701 and for females (PDf) was 0.999 999 999 999 9958. This study represents an extensive report on X chromosomal STR markers variation in the Highlander Tibetans population for forensic applications and population genetic studies.
“…Considering all aforementioned, knowledge about mutation rates and possible mutational processes of different STR loci is important for accurate genetic profiles interpretation (Qian et al, 2015;Hamester et al, 2019). As previously mentioned in the Introduction section, a number of population-genetic studies, focused on evaluating of forensic efficiency parameters of STR markers (Grskovic et al, 2013 2016; Crnjac et al, 2017;Veselinovic et al, 2018;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) indicated a high degree of reliability of these markers important for all kinds of forensic-genetic analyses, including kinship and parentage testing. Finally, results from these two cases clearly demonstrate that X-STR markers have the potential to solve parentage cases not easily solved by standard analysis based on autosomal STR markers.…”
Section: Discussionmentioning
confidence: 99%
“…Besides high heterozygosity, discriminating power, clearly defined repetitive units and precisely determined allelic variants, simple amplification and detection of STR markers make them ideal for forensic analyses (Khalil et al, 2008;Primorac et al, 2014;Gomes et al, 2020). STR markers located on the autosomal, X and Y chromosomes are highly informative, practical and reliable for all kinds of forensic-genetic analyses including parentage testing, what was strongly supported by many population-genetic studies focused on investigating allele frequencies and forensic parameters (such as heterozygosity, power of discrimination, power of exclusion, polymorphic information content, matching probability and typical paternity index) of autosomal STR (Al-Eitan and Tubaishat, 2016;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) and X-STR loci (Grskovic et al, 2013;Crnjac et al, 2017;Veselinovic et al, 2018) in different populations. Generally, parentage testing follows Mendelian inheritance law, according to which child receives one allele from the mother and the other allele from the father .…”
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“…We refer to these data as our primary data set. Additional publications [14][15][16][17][18][19][20][21][22][23][24][25] listed in Supplementary Table 1, with only cluster specific haplotypes were considered for a subset of the analyzes. We refer to these data as our secondary data set.…”
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