Genetic Analysis Method for <i>Staphylococcus chromogenes</i> Associated with Goat Mastitis

Romero, Rocío Angélica Ruiz; Cervantes-Olivares, R A; Watty, Andrés Ducoing; Martínez-Gómez, Daniel; Díaz-Aparicio, Efrén; Méndez-Olvera, Estela T.

doi:10.21307/pjm-2018-019

Cited by 3 publications

(6 citation statements)

References 32 publications

(22 reference statements)

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“…Moreover, we detected the presence of genes responsible for cell adhesion and immune evasion in these S. chromogenes isolates, including atl, capO, capD, adsA and sbi. Notably, genes encoding toxins, such as beta hemolysin (hlb), lipases (lip and geh) and thermonuclease (nuc), were also confirmed (S5B Fig), which have been identified in S. chromogenes-mediated mastitis of bovines and goats [29,30]. Taken together, our results indicate that both S. chromogenes and Av.…”

Section: Extensive Spread Of Multi-drug Resistant Av Paragallinarumsupporting

confidence: 66%

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Resident bacteria contribute to opportunistic infections of the respiratory tract

Wang

Yang

et al. 2021

PLoS Pathog

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Opportunistic pathogens frequently cause volatile infections in hosts with compromised immune systems or a disrupted normal microbiota. The commensalism of diverse microorganisms contributes to colonization resistance, which prevents the expansion of opportunistic pathogens. Following microbiota disruption, pathogens promptly adapt to altered niches and obtain growth advantages. Nevertheless, whether and how resident bacteria modulate the growth dynamics of invasive pathogens and the eventual outcome of such infections are still unclear. Here, we utilized birds as a model animal and observed a resident bacterium exacerbating the invasion of Avibacterium paragallinarum (previously Haemophilus paragallinarum) in the respiratory tract. We first found that negligibly abundant Staphylococcus chromogenes, rather than Staphylococcus aureus, played a dominant role in Av. paragallinarum-associated infectious coryza in poultry based on epidemic investigations and in vitro analyses. Furthermore, we determined that S. chromogenes not only directly provides the necessary nutrition factor nicotinamide adenine dinucleotide (NAD+) but also accelerates its biosynthesis and release from host cells to promote the survival and growth of Av. paragallinarum. Last, we successfully intervened in Av. paragallinarum-associated infections in animal models using antibiotics that specifically target S. chromogenes. Our findings show that opportunistic pathogens can hijack commensal bacteria to initiate infection and expansion and suggest a new paradigm to ameliorate opportunistic infections by modulating the dynamics of resident bacteria.

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Section: Extensive Spread Of Multi-drug Resistant Av Paragallinarumsupporting

confidence: 66%

“…Notably, genes encoding toxins, such as beta hemolysin ( hlb ), lipases ( lip and geh ) and thermonuclease ( nuc ), were also confirmed ( S5B Fig ), which have been identified in S . chromogenes -mediated mastitis of bovines and goats [ 29 , 30 ]. Taken together, our results indicate that both S .…”

Section: Resultsmentioning

confidence: 99%

Resident bacteria contribute to opportunistic infections of the respiratory tract

Wang

Yang

et al. 2021

PLoS Pathog

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“…RT-PCR conditions for amplification consist of denaturation at 94 °C for 10 s, anealing at 60 °C for 15 s, and extension at 72 °C for 10 s. The whole reaction was maintained for 40 amplification cycles . The target gene expression was normalized to GAPDH gene expression, and the relative gene expression was evaluated using the 2 –ΔΔCt method . The information about primers is listed in Table .…”

Section: Materials and Methodsmentioning

confidence: 99%

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Huang

Chen

Yao

et al. 2023

J. Agric. Food Chem.

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Ovotransferrin (OVT) has been confirmed to have anti-inflammatory activity. However, its effect and mechanism on gastric inflammation are unclear. In this study, the effect and mechanism of the OVT on the tumor necrosis factor-α (TNF-α) induced inflammatory response in gastric epithelial cells (GES-1) were investigated. The enzyme linked immunosorbent assay (ELISA) was used to determine the levels of inflammation cytokines, followed by RNA sequencing to explore the potential pathways of its anti-inflammatory effect, and then it was validated by Western blotting and pathways inhibitors. Results showed that the OVT at concentrations of 50–400 μg/mL displayed nontoxicity against GES-1 cells. Additionally, 100 μg/mL of OVT obviously reduced the secretion of interleukin (IL)-8, IL-6, and TNF-α by 63.02% (630.09/1703.98), 35.53% (935.81/1451.43), and 36.19% (964.60/1511.63), respectively. The results of RNA sequencing exhibited that the OVT significantly influences the activation of mitogen-activated protein kinase (MAPK) and the nuclear factor kappa-light-chain enhancer of activated B cell (NF-κB) pathways, which was verified by the levels of p-IKK, p-IκB, p-P65, p-ERK, p-JNK, and p-P38 protein. IL-8 contents released by GES-1 cells after incubation with inhibitors of NF-κB and MAPK pathways further confirmed that OVT hindered activation of these two pathways. Collectively, these results suggested that OVT was a natural protein with the potential to treat gastric inflammation.

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“…Previous studies have implicated S. chromogenes as a frequent cause of intramammary infections in cattle on dairy farms and have demonstrated strain diversity can vary within and between farms. However the DNA fragment profiling methods used in these studies to characterize strain diversity, notably PFGE, ALPF, RAPD-PCR, and MLVA, have yielded conflicting estimates of diversity, ranging from a high degree of genetic conservation to considerable genetic heterogeneity [10,11,18,19,22,23,[44][45][46][47][48][49]. Given the stand-alone nature of these studies and the incompatibility of their measures of strain diversity, the results of different studies cannot be directly compared or consolidated.…”

Section: Genetic Diversity In S Chromogenesmentioning

confidence: 99%

“…Pulsed field gel electrophoresis (PFGE), ribotyping, amplification fragment length polymorphism (AFLP), random amplification of polymorphic DNA (RAPD)-PCR, and multiple locus variable-number tandem repeat analysis (MLVA) have been used to discriminate between S . chromogenes strains in epidemiological studies [ 10 , 11 , 15 , 18 , 19 , 22 , 23 ]; these methods demonstrate sufficient discriminatory power in short-term epidemiological investigations. However, DNA fragment electrophoretic band pattern (i.e.…”

Section: Introductionmentioning

confidence: 99%

Characterization of genetic diversity and population structure within Staphylococcus chromogenes by multilocus sequence typing

et al. 2021

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Staphylococcus chromogenes is a common skin commensal in cattle and has been identified as a frequent cause of bovine mastitis and intramammary infections. We have developed a seven locus Multilocus Sequence Typing (MLST) scheme for typing S. chromogenes. Sequence-based typing systems, such as MLST, have application in studies of genetic diversity, population structure, and epidemiology, including studies of strain variation as a factor in pathogenicity or host adaptation. The S. chromogenes scheme was tested on 120 isolates collected from three geographic locations, Vermont and Washington State in the United States and Belgium. A total of 46 sequence types (STs) were identified with most of the STs being location specific. The utility of the typing scheme is indicated by a discrimination power of 95.6% for all isolates and greater than 90% for isolates from each of the three locations. Phylogenetic analysis placed 39 of the 46 STs into single core group consistent with a common genetic lineage; the STs in this group differ by less than 0.5% at the nucleotide sequence level. Most of the diversification in this lineage group can be attributed to mutation; recombination plays a limited role. This lineage group includes two clusters of single nucleotide variants in starburst configurations indicative of recent clonal expansion; nearly 50% of the isolates sampled in this study are in these two clusters. The remaining seven STs were set apart from the core group by having alleles with highly variable sequences at one or more loci. Recombination had a higher impact than mutation in the diversification of these outlier STs. Alleles with hypervariable sequences were detected at five of the seven loci used in the MLST scheme; the average sequence distances between the hypervariable alleles and the common core alleles ranged from 12 to 34 nucleotides. The extent of these sequence differences suggests the hypervariable alleles may be remnants of an ancestral genotype.

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Genetic Analysis Method for Staphylococcus chromogenes Associated with Goat Mastitis

Cited by 3 publications

References 32 publications

Resident bacteria contribute to opportunistic infections of the respiratory tract

Resident bacteria contribute to opportunistic infections of the respiratory tract

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Characterization of genetic diversity and population structure within Staphylococcus chromogenes by multilocus sequence typing

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