Characterization of genetic diversity and population structure within Staphylococcus chromogenes by multilocus sequence typing

Huebner, Rebeca; Mugabi, Robert; Hetesy, Gabriella; Fox, Lawrence K.; Vliegher, Sarne De; Visscher, Alex De; Barlow, John W.; Sensabaugh, George F.

doi:10.1371/journal.pone.0243688

Cited by 12 publications

(5 citation statements)

References 51 publications

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“…We noticed that strains associated with bovine mastitis clustered together as separate subclades in all the NASM genomes. Previous studies have reported that S. chromogenes ST-1 and ST-6 strains are associated with bovine mastitis (Huebner et al, 2021) (Persson Waller et al, 2023). Our observation also documents that S. chromogenes ST-1 and ST-6 are associated with bovine mastitis.…”

Section: Discussionsupporting

confidence: 86%

Genome sequencing and comparative genomic analysis of bovine mastitis-associated non-aureus staphylococci and mammaliicocci (NASM) strains from India

Ramesh,

Sivakumar,

Annamanedi

et al. 2024

Preprint

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Bovine mastitis is a significant issue causing severe economic losses in the global dairy industry, affecting animal well-being and production. Non-aureus staphylococci and mammaliicocci (NASM) are the predominant group of pathogens responsible for mastitis in dairy cattle. Understanding the prevalence of virulence factors and antimicrobial resistance among these pathogens is essential to unravel the molecular epidemiology of mastitis, and it is best accomplished through whole-genome sequencing (WGS). In this study, we describe the WGS and comparative genomic analysis of 22 mastitis-associated NASM strains isolated from India. The mean genome size of the strains was 2.55 Mbp, with an average GC content of 32.2%. We identified 14 different sequence types (STs) among the 22 NASM strains. Of these, ST1 and ST6 of S. chromogenes were exclusively associated with bovine mastitis. Genome-wide SNP-based minimum spanning tree revealed the intricate phylogenetic relationships among NASM strains from India, categorizing them into five major clades. Interestingly, mastitis-associated strains formed separate subclades in all the NASM species studied, indicating distinct host-specific co-evolution. The study identified 32 antimicrobial resistance (AMR) genes and 53 virulence-associated genes, providing insights into the genetic factors which could potentially contribute to the pathogenicity of NASM species. Some virulence and AMR genes were found in the predicted genomic islands, suggesting possible horizontal transfer events.

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Section: Discussionsupporting

confidence: 86%

Genome sequencing and comparative genomic analysis of bovine mastitis-associated non-aureus staphylococci and mammaliicocci (NASM) strains from India

Ramesh,

Sivakumar,

Annamanedi

et al. 2024

Preprint

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“…DNA was extracted from the first and last, when present, collected S. chromogenes isolate cultured from a quarter with a commercially available kit (DNeasy Blood and Tissue kit, Qiagen, Venlo, The Netherlands). The DNA was submitted to multilocus sequence typing (MLST) [42] to verify whether the recovered S. chromogenes isolates belonged to the same type as the inoculated S. chromogenes IM (ST 1). Four S. chromogenes isolates with a known sequence type (one ST1, two ST 44 and one ST 18) served as controls.…”

Section: Maldi-tof Mass Spectrometry and Multilocus Sequence Typingmentioning

confidence: 99%

Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Beuckelaere

Visscher

Souza

et al. 2021

Vet Res

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Although extensive research has been performed on bovine non-aureus staphylococci (NAS), several aspects such as bacteria-host interaction remain largely unstudied. Moreover, only a few mastitis pathogen challenge studies in cows have been conducted in the dry period, an important period that allows intramammary infection (IMI) to cure and new IMI to occur. We challenged 16 quarters of 4 Holstein Friesian cows at dry off with 100; 100 000 or 10 000 000 CFU of the udder-adapted S. chromogenes IM strain. Four quarters from one cow served as negative controls. Internally sealed quarters remained untouched, whereas non-sealed quarters were sampled 3 times during the dry period. After parturition, colostrum and daily milk samples were taken during the first week of lactation of all quarters. In total, 8 quarters appeared to be colonized, since S. chromogenes IM was recovered at least once during the experiment, as substantiated using Multilocus Sequence Typing. S. chromogenes IM shedding was highest in dry quarters inoculated with 10 000 000 CFU. Colonized quarters had the highest quarter somatic cell count (qSCC) in early lactation. Inoculated quarters (both colonized and non-colonized) had lower IL-6 and IL-10 concentrations in the dry period, whilst IFN-γ levels tended to be higher in colonized quarters compared to non-inoculated quarters. Also, IgG2 levels were higher in inoculated compared to non-inoculated quarters and the IgG2/IgG1 ratio was on average above 1. To conclude, we showed that dry quarters can be colonized with S. chromogenes IM, resulting in a shift towards a Th1 response in late gestation and early lactation characterised by an increased IgG2 concentration. However, further research is needed to confirm our findings.

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“…Staphylococcus hyicus is the major causative agent of piglets’ exudative epidermitis [ 101 ]. Staphylococcus chromogenes has been identified as a frequent cause of bovine mastitis and intramammary infections [ 102 , 103 ]. Interestingly, colonization of mice with Staphylococcus epidermidis, a skin commensal bacteria producing 6-N-hydroxyaminopurine (6-HAP), has reduced the incidence of ultraviolet-induced tumours [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis of skin microbiome and gut microbiome in melanoma progression

et al. 2022

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Background The microbiome alterations are associated with cancer growth and may influence the immune system and response to therapy. Particularly, the gut microbiome has been recently shown to modulate response to melanoma immunotherapy. However, the role of the skin microbiome has not been well explored in the skin tumour microenvironment and the link between the gut microbiome and skin microbiome has not been investigated in melanoma progression. Therefore, the aim of the present study was to examine associations between dysbiosis in the skin and gut microbiome and the melanoma growth using MeLiM porcine model of melanoma progression and spontaneous regression. Results Parallel analysis of cutaneous microbiota and faecal microbiota of the same individuals was performed in 8 to 12 weeks old MeLiM piglets. The bacterial composition of samples was analysed by high throughput sequencing of the V4-V5 region of the 16S rRNA gene. A significant difference in microbiome diversity and richness between melanoma tissue and healthy skin and between the faecal microbiome of MeLiM piglets and control piglets were observed. Both Principal Coordinate Analysis and Non-metric multidimensional scaling revealed dissimilarities between different bacterial communities. Linear discriminant analysis effect size at the genus level determined different potential biomarkers in multiple bacterial communities. Lactobacillus, Clostridium sensu stricto 1 and Corynebacterium 1 were the most discriminately higher genera in the healthy skin microbiome, while Fusobacterium, Trueperella, Staphylococcus, Streptococcus and Bacteroides were discriminately abundant in melanoma tissue microbiome. Bacteroides, Fusobacterium and Escherichia-Shigella were associated with the faecal microbiota of MeLiM piglets. Potential functional pathways analysis based on the KEGG database indicated significant differences in the predicted profile metabolisms between the healthy skin microbiome and melanoma tissue microbiome. The faecal microbiome of MeLiM piglets was enriched by genes related to membrane transports pathways allowing for the increase of intestinal permeability and alteration of the intestinal mucosal barrier. Conclusion The associations between melanoma progression and dysbiosis in the skin microbiome as well as dysbiosis in the gut microbiome were identified. Results provide promising information for further studies on the local skin and gut microbiome involvement in melanoma progression and may support the development of new therapeutic approaches.

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Characterization of genetic diversity and population structure within Staphylococcus chromogenes by multilocus sequence typing

Cited by 12 publications

References 51 publications

Genome sequencing and comparative genomic analysis of bovine mastitis-associated non-aureus staphylococci and mammaliicocci (NASM) strains from India

Genome sequencing and comparative genomic analysis of bovine mastitis-associated non-aureus staphylococci and mammaliicocci (NASM) strains from India

Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Dysbiosis of skin microbiome and gut microbiome in melanoma progression

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