Genetic analysis and molecular characterisation of laboratory and field mutants of <i>Botryotinia fuckeliana</i> (<i>Botrytis cinerea</i>) resistant to QoI fungicides

Angelini, Rita Milvia De Miccolis; Rotolo, Caterina; Masiello, Mario; Pollastro, Stefania; Ishii, Hideo; Faretra, F.

doi:10.1002/ps.3281

Cited by 30 publications

(28 citation statements)

References 51 publications

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“…, second round). These results suggested that the repeated introduction of QoI fungicide selection pressure increased the frequency of G143A‐mutated cytochrome b genes in the B. cinerea mitochondrial population, as reported previously …”

Section: Resultsmentioning

confidence: 99%

Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi-quantitative AS-PCR assay

et al. 2014

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Section: Resultsmentioning

confidence: 99%

Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi-quantitative AS-PCR assay

et al. 2014

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“…The PCR amplifications were conducted using the parameters described by Jiang et al (2009). The PCR products were examined by electrophoresis on a 1.5% agarose gel in 1× Tris-acetate-EDTA (TAE) buffer (De Miccolis Angelini et al, 2012).…”

Section: Genetic Characterizationmentioning

confidence: 99%

“…Moreover, field resistance to novel succinate dehydrogenase inhibitor (SDHI) fungicides was detected soon after their introduction (Bardas et al, 2010). Molecular characterization of B. cinerea isolates that were sensitive or resistant to single-site fungicides showed the involvement of major genes of the fungus and a strong association between resistant phenotypes and point mutations (single nucleotide polymorphisms, SNPs) (De Miccolis Angelini et al, 2012). These results have been observed for several groups of fungicides, including SDHIs (Yin et al, 2012), SBIs-III (Fillinger et al, 2008) and quinone outside inhibitors (QoIs) (De Miccolis Angelini et al, 2012;Ishii et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Grez¹,

Copier²,

Román³

et al. 2017

Ciencia e investigación agraria

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were sensitive to all fungicides tested; from north to south, the frequency of sensitive isolates in the regions of Valparaiso, Metropolitana and O'Higgins ranged from 48.15% to 21.1% and 5.88%, respectively. Four hundred and twenty isolates (79%) showed resistance to single or multiple fungicides, 134 (25.4%) were simultaneously resistant to azoxystrobin and pyrimethanil. No fludioxonil-resistant isolates were found, indicating that fludioxonil has great potential for gray mold control in table grapes in Chile. From sixty randomly selected B. cinerea isolates, only the azoxystrobin-resistant isolates carried the G143A point mutation; according to the cytochrome b (cyt b) gene structure, the third intron Bcbi-143/144 was only detected in the azoxystrobin-sensitive isolates. The H272R and H272Y point mutations in the succinate dehydrogenase subunit B (sdhB) gene were associated only with the boscalid-resistant isolates. The F412S and F412V point mutations were found in the sequenced erg27 gene of randomly selected fenhexamid-resistant isolates. These results contribute to the knowledge of B. cinerea fungicide resistance for table grape vine crops in Central Chile, particularly for the development of multiple-resistance and the associated resistance mechanisms of azoxystrobin, boscalid and fenhexamid-resistant isolate populations. Antiresistance strategies are discussed in a general manner.

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“…The screening for resistant A143 alleles directly from CFUs was found suitable for detecting resistance. In vitro a loss of the resistance might be expected even from the first transfer of the culture in fungicide‐free media (De Miccolis Angelini et al ., ). High resistance to anilinopyrimidine fungicides (AP) has also detected in B. cinerea isolates.…”

Section: Resultsmentioning

confidence: 97%

Development of a two-step high-resolution melting (HRM) analysis for screening sequence variants associated with resistance to the QoIs, benzimidazoles and dicarboximides in airborne inoculum ofBotrytis cinerea

Chatzidimopoulos

Ganopoulos

Vellios

et al. 2014

FEMS Microbiol Lett

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A rapid, high-resolution melting (HRM) analysis protocol was developed to detect sequence variations associated with resistance to the QoIs, benzimidazoles and dicarboximides in Botrytis cinerea airborne inoculum. HRM analysis was applied directly in fungal DNA collected from air samplers with selective medium. Three and five different genotypes were detected and classified according to their melting profiles in BenA and bos1 genes associated with resistance to benzimidazoles and dicarboximides, respectively. The sensitivity of the methodology was evident in the case of the QoIs, where genotypes varying either by a single nucleotide polymorphism or an additional 1205-bp intron were separated accurately with a single pair of primers. The developed two-step protocol was completed in 82 min and showed reduced variation in the melting curves' formation. HRM analysis rapidly detected the major mutations found in greenhouse strains providing accurate data for successfully controlling grey mould.

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Genetic analysis and molecular characterisation of laboratory and field mutants of Botryotinia fuckeliana (Botrytis cinerea) resistant to QoI fungicides

Cited by 30 publications

References 51 publications

Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi-quantitative AS-PCR assay

Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi-quantitative AS-PCR assay

Frequency of fungicide-resistant Botrytis cinerea populations isolated from ‘Thompson Seedless’ table grapes in the Central Valley of Chile

Development of a two-step high-resolution melting (HRM) analysis for screening sequence variants associated with resistance to the QoIs, benzimidazoles and dicarboximides in airborne inoculum ofBotrytis cinerea

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