Genes that control a temperature-compensated ultradian clock in Caenorhabditis elegans.

Iwasaki, Kouichi; Liu, Dennis W. C.; Thomas, James H.

doi:10.1073/pnas.92.22.10317

Cited by 82 publications

(67 citation statements)

References 26 publications

(6 reference statements)

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“…It was later noted that these mutants are also resistant to high osmolarity (Osr) (K. Mase, Y. Ohshima and M. Koga, personal communication). dec-2(sa89) was identified in a separate screen for mutants with abnormal defecation cycle period (Dec) (Iwasaki et al 1995). osm-7(n1515) and dec-2(sa89) are alleles of the same gene; thus we refer to this gene as osm-7 for the remainder of the article.…”

Section: Resultsmentioning

confidence: 99%

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Identification of a Novel Gene Family Involved in Osmotic Stress Response inCaenorhabditis elegans

2006

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Organisms exposed to the damaging effects of high osmolarity accumulate solutes to increase cytoplasmic osmolarity. Yeast accumulates glycerol in response to osmotic stress, activated primarily by MAP kinase Hog1 signaling. A pathway regulated by protein kinase C (PKC1) also responds to changes in osmolarity and cell wall integrity. C. elegans accumulates glycerol when exposed to high osmolarity, but the molecular pathways responsible for this are not well understood. We report the identification of two genes, osm-7 and osm-11, which are related members of a novel gene family. Mutations in either gene lead to high internal levels of glycerol and cause an osmotic resistance phenotype (Osr). These mutants also have an altered defecation rhythm (Dec). Mutations in cuticle collagen genes dpy-2, dpy-7, and dpy-10 cause a similar Osr Dec phenotype. osm-7 is expressed in the hypodermis and may be secreted. We hypothesize that osm-7 and osm-11 interact with the cuticle, and disruption of the cuticle causes activation of signaling pathways that increase glycerol production. The phenotypes of osm-7 are not suppressed by mutations in MAP kinase or PKC pathways, suggesting that C. elegans uses signaling pathways different from yeast to mount a response to osmotic stress.

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Section: Resultsmentioning

confidence: 99%

“…Mapping and transgenic rescue: osm-7(sa89) was previously mapped to the right arm of chromosome III by standard mapping crosses (Iwasaki et al 1995). Deletion mapping was carried out by crossing osm-7(sa89) to GE2175 and GE2204, strains carrying the deletions tDf6 and tDf10, respectively.…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Gene Family Involved in Osmotic Stress Response inCaenorhabditis elegans

2006

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“…elegans were maintained using standard culture methods (Lewis and Fleming, 1995). The following strains were used: Bristol N2 wild-type; JT73 (itr-1 (sa73) IV, Iwasaki et al, 1995); CB1091 (unc-13 (e1091) I, Waterston and Brenner, 1978); BA1 (fer-1 (hc1) I, Ward and Miwa, 1978); CB1467 (him-5 (e1467) V, Hodgkin et al, 1979); CB1489 (him-8 (e1489) IV, Hodgkin et al, 1979);and CB138 (unc-24 (e138) IV, Brenner, 1974). HB100 (itr-1 (sa73); him-5 (e1467)) was constructed by mating CB1467 males and JT73 hermaphrodites.…”

Section: Elegans Strains and Culturementioning

confidence: 99%

Inositol 1,4,5-Trisphosphate Signaling Regulates Mating Behavior inCaenorhabditis elegansMales

Gower

Walker

Baylis

2005

MBoC

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Complex behavior requires the coordinated action of the nervous system and nonneuronal targets. Male mating in Caenorhabditis elegans consists of a series of defined behavioral steps that lead to the physiological outcomes required for successful impregnation. We demonstrate that signaling mediated by inositol 1,4,5-trisphosphate (IP 3 ) is required at several points during mating. Disruption of IP 3 receptor (itr-1) function results in dramatic loss of male fertility, due to defects in turning behavior (during vulva location), spicule insertion and sperm transfer. To elucidate the signaling pathways responsible, we knocked down the six C. elegans genes encoding phospholipase C (PLC) family members. egl-8, which encodes PLC-␤, functions in spicule insertion and sperm transfer. itr-1 and egl-8 are widely expressed in the male reproductive system. An itr-1 gain-of-function mutation rescues infertility caused by egl-8 RNA interference, indicating that egl-8 and itr-1 function together as central components of the signaling events controlling sperm transfer.

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“…Indeed, temperature compensation is often cited as an essential property of circadian and ultradian rhythms. [5][6][7][8][9][10] Cases where the temperature coefficient is considerably smaller than 1 are referred to as overcompensated and reflect the counterintuitive situation in which the process becomes slower as the temperature increases. Such behavior would result, as seen in eq 3, in a negative activation energy; whereas when q 10 ∼ 1, the activation energy approaches zero.…”

Section: Introductionmentioning

confidence: 99%

Temperature (Over)Compensation in an Oscillatory Surface Reaction

et al. 2008

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Biological rhythms are regulated by homeostatic mechanisms that assure that physiological clocks function reliably independent of temperature changes in the environment. Temperature compensation, the independence of the oscillatory period on temperature, is known to play a central role in many biological rhythms, but it is rather rare in chemical oscillators. We study the influence of temperature on the oscillatory dynamics during the catalytic oxidation of formic acid on a polycrystalline platinum electrode. The experiments are performed at five temperatures from 5 to 25°C, and the oscillations are studied under galvanostatic control. Under oscillatory conditions, only non-Arrhenius behavior is observed. Overcompensation with temperature coefficient (q 10 , defined as the ratio between the rate constants at temperature T + 10°C and at T) < 1 is found in most cases, except that temperature compensation with q 10 ≈ 1 predominates at high applied currents. The behavior of the period and the amplitude result from a complex interplay between temperature and applied current or, equivalently, the distance from thermodynamic equilibrium. High, positive apparent activation energies were obtained under voltammetric, nonoscillatory conditions, which implies that the non-Arrhenius behavior observed under oscillatory conditions results from the interplay among reaction steps rather than from a weak temperature dependence of the individual steps.

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Genes that control a temperature-compensated ultradian clock in Caenorhabditis elegans.

Cited by 82 publications

References 26 publications

Identification of a Novel Gene Family Involved in Osmotic Stress Response inCaenorhabditis elegans

Identification of a Novel Gene Family Involved in Osmotic Stress Response inCaenorhabditis elegans

Inositol 1,4,5-Trisphosphate Signaling Regulates Mating Behavior inCaenorhabditis elegansMales

Temperature (Over)Compensation in an Oscillatory Surface Reaction

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