Genes Related to Suppression of Malignant Phenotype Induced by Maitake D-Fraction in Breast Cancer Cells

Alonso, Ernesto; Orozco, Manuela; Nieto, A.E.; Balogh, Gabriela Andrea

doi:10.1089/jmf.2012.0222

Cited by 25 publications

(37 citation statements)

References 87 publications

(102 reference statements)

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“…With the objective to determine if Maitake D‐Fraction PRO4X modifies the gene expression of tumoral phenotype, total RNA was isolated from all breast tumors of experimental groups and from the mammary glands of non‐tumor‐bearing mice from Maitake Pro4X treatment. ABCG2, CUL3, IGFBP5, PTEN, and SPACR gene expressions were modified after Maitake treatment in MCF‐7 cells as were previously published by us . Figure A shows the RT‐PCR bands in the agarose gels from each of those genes in breast tumors after treatment with or without Maitake.…”

Section: Resultsmentioning

confidence: 99%

“…Later studies were performed to determine whether the administration of Maitake Pro4X‐modulated gene expression on breast tumor tissue. ABCG2, Cul3, IGFBPR5, PTEN, and SPARC genes levels of expression were related to the malignant phenotype in tumor cells, and those differential expressions were found to be modulated by Maitake in our previous studies .…”

Section: Discussionmentioning

confidence: 99%

“…Our previous data support the concept that Maitake D‐Fraction can influence the switching on and off of genes expressed in human breast cancer MCF‐7 cells, and thus could be able to control the breast cancer phenotype inducing apoptosis and probably involved in the reversion of the malignant phenotype .…”

Section: Introductionmentioning

confidence: 99%

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Maitake Pro4X has anti‐cancer activity and prevents oncogenesis in BALBc mice

et al. 2016

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The understanding of the molecular mechanisms of the immune tolerance induced by the tumoral microenvironment is fundamental to prevent cancer development or to treat cancer patients using immunotherapy. Actually, there are investigations about “addressed‐drugs” against cancer cells without affecting normal cells. It could be ideal to find selective and specific compounds that only recognize and destroy tumor cells without damaging the host normal cells. For thousands of years, mushrooms have been used for medicinal purposes because of their curative properties. D‐Fraction, an extract of Maitake (from the edible Grifola frondosa mushroom), rich in β‐glucans, exert notable effects in the immune system. Until now, some published articles suggest that Maitake D‐Fraction could have anti‐tumoral activity, prevent oncogenesis and metastasis in some tumor types. However, there are no clear data about Maitake D‐Fraction action on breast cancer prevention and its exact molecular mechanisms are not yet elucidated. The experiments were performed employing 25 female BALBc mice that were treated with and without Maitake D‐Fraction Pro4X or Maitake Standard for 15 days by daily intraperitoneal injection. After treatment period, all mice were implanted with murine tumor cells LM3 to induce mammary tumorigenesis. Animals were checked weekly and killed after 46 days of LM3 transplant; percentage of cancer prevention, rate of tumor growing, and overall survival were determined. Under dissection, the internal organs were evaluated histologically and genetically by RT‐PCR. We found that 5 mg/kg per day of Maitake D‐Fraction Pro4X, administered dairy during 15 days to BALBc mice was able to block more than 60% breast cancer development. However, Maitake Standard prevents oncogenesis in 26% to respect control. In this work, we found that Maitake D‐Fraction Pro4X, administered to BALBc mice, prevents breast carcinogenesis, block tumor invasiveness, reduce angiogenesis, and increase overall survival.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Maitake Pro4X has anti‐cancer activity and prevents oncogenesis in BALBc mice

et al. 2016

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“…So far, we have demonstrated that the treatment with Maitake D-Fraction Pro4X prevents the development of mammary tumorigenesis, blocks tumor invasiveness, reduce tumor angiogenesis, increases overall survival in animals, and exhibits selective cytotoxicity [24][25][26]. Moreover, we also demonstrated that the use of Maitake D-Fraction Pro4X is safe and nontoxic as well.…”

Section: Introductionmentioning

confidence: 70%

“…We choose genes such as ABCG2, CUL3, IGFBP5, PTEN, and SPACR, whose expressions were modiied after Maitake treatment in MCF-7 cells previously published [25]. For this purpose, total RNA were isolated and after puriication RT-PCR were performed in each breast tumor tissue from control, Maitake Standard, and Maitake Pro4X groups.…”

Section: Efect Of Maitake Pro4x On Speciic Gene Expression Related Tomentioning

confidence: 99%

Immunotherapeutic and Preventive Role of Purified Extract Rich in Beta-Glucans Derived from D-Fraction of Grifola frondosa Mushroom in Experimental Mice Biomodel of Mammary Carcinogenesis

Braico¹,

Máximo²,

Balogh³

2017

Breast Cancer - From Biology to Medicine

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The overall vision of the modern science needs to change to a revalorization of the natural compounds and their beneicial efects on human diseases, such as cancer. Medicinal mushrooms have been used since thousands of years due to its healing properties. Maitake (Grifola frondosa) is presented as one of the most interesting medicinal mushrooms that have been studied. Until now, Maitake D-Fraction may have anticarcinogenic activity, preventing oncogenesis and metastasis in certain tumor types. However, the exact molecular mechanism by which D-Fraction acts are yet unknown. The results shown in this chapter suggest that Maitake D-Fraction Pro4X, administered intraperitoneally, prevents signiicantly the development of mammary tumorigenesis, increases survival, and reduces the process of angiogenesis in BALBc mice. Although yet to determine the active component of the extract and the molecular mechanism by which it operates in the breast carcinogenesis process. The socioeconomic impact of this research project could be important, considering that in Argentina similar studies using natural compounds derived from medicinal mushrooms for cancer therapy have not yet been performed. The beneicial efects of Maitake, if proven, could be useful for the treatment of cancer patients who are undergoing chemotherapy or radiation or for breast cancer prevention in high-risk population.

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Methylation‐independent ITGA2 overexpression is associated with poor prognosis in de novo acute myeloid leukemia

Lian

Zhang

et al. 2018

Journal Cellular Physiology

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Previous studies have been indicated that integrin α2 (ITGA2) may be important in cell migration, invasion, survival, and angiogenesis. However, the correlation between ITGA2 expression and acute myeloid leukemia (AML) is still unclear. Real-time quantitative polymerase chain reaction was carried out to analyze ITGA2 messenger RNA level. Methylation-specific polymerase chain reaction (PCR) and bisulfite sequencing PCR were performed to detect the methylation of ITGA2 promoter. ITGA2 expression was significantly upregulated in 134 de novo AML patients compared with 33 controls (p = 0.007). ITGA2 group had markedly lower complete remission (CR) rate than ITGA2 group (p = 0.011). Furthermore, the overall survival in ITGA2 patients was significantly shorter than ITGA2 patients throughout AML cohort, non-acute promyelocytic leukemia (APL) and cytogenetic normal-AML (p = 0.001, 0.002, and 0.044, respectively). Multivariate analysis confirmed that ITGA2 overexpression served as an independent prognostic factor in both whole-cohort AML patients (p = 0.018) and non-APL AML patients (p = 0.021). Besides, ITGA2 expression level was significantly decreased in AML patients after CR (p = 0.011), and was returned at the time of relapse phase (p = 0.021). Moreover, unmethylated ITGA2 promoter was identified in normal controls, leukemia cell lines, and primary leukemia cells with low or high ITGA2 expression. In conclusions, methylation-independent ITGA2 overexpression is associated with poor prognosis in AML.

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Genes Related to Suppression of Malignant Phenotype Induced by Maitake D-Fraction in Breast Cancer Cells

Cited by 25 publications

References 87 publications

Maitake Pro4X has anti‐cancer activity and prevents oncogenesis in BALBc mice

Maitake Pro4X has anti‐cancer activity and prevents oncogenesis in BALBc mice

Immunotherapeutic and Preventive Role of Purified Extract Rich in Beta-Glucans Derived from D-Fraction of Grifola frondosa Mushroom in Experimental Mice Biomodel of Mammary Carcinogenesis

Methylation‐independent ITGA2 overexpression is associated with poor prognosis in de novo acute myeloid leukemia

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