Genes Encoding Proteins Homologous to Halobacterial Gvps N, J, K, F &amp; L are Located Downstream of<i>gvpC</i>in the Cyanobacterium<i>Anabaena flos-aquae</i>

Kinsman, Rachel; Hayes, P.K.

doi:10.3109/10425179709020156

Cited by 25 publications

(22 citation statements)

References 30 publications

(19 reference statements)

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“…there are 10 different gvp genes in a linear cluster gvpA, C, N, J, X, K, F/L, G, V, W (Mlouka et al, 2004;Dunton & Walsby, 2005). In A. flos-aquae, nine of the same genes are found in the same order, but one, gvpX, is absent (see Kinsman & Hayes, 1997, and GenBank accession no. U17109).…”

Section: Introductionmentioning

confidence: 99%

Analysis of tryptic digests indicates regions of GvpC that bind to gas vesicles of Anabaena flos-aquae

et al. 2006

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The gas vesicles of the cyanobacterium Anabaena flos-aquae contain two main proteins: GvpA, which forms the ribs of the hollow cylindrical shell, and GvpC, which occurs on the outer surface. Analysis by MALDI-TOF MS shows that after incubating Anabaena gas vesicles in trypsin, GvpA was cleaved only at sites near the N-terminus, whereas GvpC was cleaved at most of its potential tryptic sites. Many of the resulting tryptic peptides from GvpC remained attached to the underlying GvpA shell: the pattern of attachment indicated that there are binding sites to GvpA at both ends of the 33-residue repeats (33RRs) in GvpC, although one of the tryptic peptides within the 33RR did not remain attached. Tryptic peptides near the two ends of the GvpC molecule were also lost. The mean critical collapse pressure of Anabaena gas vesicles decreased from 0.63 MPa to 0.20 MPa when GvpC was removed with urea or fully digested with trypsin; partial digestion resulted in partial decrease in critical pressure.

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Section: Introductionmentioning

confidence: 99%

Analysis of tryptic digests indicates regions of GvpC that bind to gas vesicles of Anabaena flos-aquae

et al. 2006

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“…The third copy of gvpC is followed by the gvpN and gvpJ genes. A similar order of the last copies of gvpA and gvpC and of gvpN and gvpJ has been found in other cyanobacterial genera, such as Anabaena (Kinsman & Hayes, 1997), Pseudanabaena (Albouy et al, 2001) and Microcystis (Mlouka et al, 2004).…”

Section: Discussionmentioning

confidence: 75%

“…GvpA and gvpC are not the only genes involved in gas vesicle formation. In cyanobacteria up to eight other gas vesicle genes have been identified (Kinsman & Hayes, 1997;Albouy et al, 2001;Mlouka et al, 2004). In the case of halophilic archaea as many as 12 additional genes have been found (DasSarma & Arora, 1997;Pfeifer et al, 1997).…”

Section: Introductionmentioning

confidence: 98%

“…PCC 6901 (CAC32465) are similar in length (127 and 126 residues, respectively) and show the highest percentage of identity among the cyanobacterial sequences of this gene presently known. The GvpJs found in other cyanobacteria are homologous to GvpA (Kinsman & Hayes, 1997;Mlouka et al, 2004). In the case of GvpJ and GvpA of Arthrospira sp.…”

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confidence: 98%

“…The Arthrospira GvpN contains a putative nucleotide binding site, LRGPAGTGKT, previously reported in cyanobacterial and halobacterial counterparts (Pfeifer & Englert, 1992;DasSarma & Arora, 1997;Kinsman & Hayes, 1997;Albouy et al, 2001;Mlouka et al, 2004).…”

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Elucidation of the gas vesicle gene clusters in cyanobacteria of the genusArthrospira(Oscillatoriales, Cyanophyta) and correlation with ITS phylogeny

Miklaszewska

Waleron

Morin

et al. 2012

European Journal of Phycology

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The genus Arthrospira comprises filamentous cyanobacteria in which the trichomes form an open helix and contain gas vacuoles. The gas vesicle gene cluster of five Arthrospira strains was amplified by PCR and sequenced. The genes are organized in one operon, in the order gvpA1-gvpC1-gvpA2-gvpC2-gvpA3-gvpC3-gvpN. In Arthrospira sp. strain PCC 8005, the genes gvpJ, gvpK, gvpV and gvpW were also identified. Each of the three copies of gvpA encodes a protein of 71 amino acids. In the case of gvpC, there are two different length variants. Each of the two shorter genes, gvpC1 and gvpC2, encodes a putative protein of 151 amino acids, while the longer one, gvpC3, codes for a putative protein of 284 residues. The amino acid sequences of GvpC1 and GvpC2 are identical to the N-terminal part of GvpC3. In spite of the presence of stop codons downstream of gvpC1 and gvpC2, the deduced amino acid sequences in these regions are highly similar to the C-terminal part of GvpC3 (residues 160 to 229). The GvpC1, GvpC2 and GvpC3 proteins contain contiguous repeats of 33 amino acids as previously reported for other cyanobacteria. The sequences of the gvpA1, gvpC1, gvpA2 and gvpC2 genes were not found in the genome data of Arthrospira sp. PCC 8005, A. maxima CS-328, and A. platensis NIES-39 as a result of incomplete assembly. The genes gvpN and gvpJ located downstream of gvpC3, encode putative proteins of 394 and 127 amino acids, respectively. The deduced amino acid sequences of gvpK, gvpV and gvpW contain 151, 112 and 227 residues, respectively. The analysis of gvp sequences of five strains of Arthrospira revealed the presence of polymorphic positions, which distinguished the strains in agreement with their previous assignments to ITS clusters I and II. This is the first report of gvp genes in members of the genus Arthrospira.

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Microscopic, molecular, and biochemical investigations to characterize a benthic cyanoprokaryote Leptolyngbya strainfrom Egypt

Semary

2012

Microscopy Res & Technique

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Microscopic, molecular, and biochemical investigations were conducted to describe a benthic mat-forming Leptolyngbya isolate collected from wastewater canal in Helwan area, Egypt. Microscopic examination revealed that the isolate was filamentous, nonheterocystous, with obvious granular surface ornamentation. Electron microscopy was used to reveal the isolate's ultrastructure. Cross walls were thick with uneven deposition. Thylakoids were convoluted and irregularly distributed. Granular content differed from one cell to the other probably due to their physiological stages/position within the filaments and/or their age. Nycridial cells were present. Highly refractile gas vesicle-like structures were detected and their identity as gas vesicles was confirmed by amplifying the gene coding for the gas vesicle protein GvpA. The presence of gas vesicles in benthic microorganisms is intriguing, and it is possible that those vesicles serve as a floating and dispersal mechanism as they increase in filaments that are about to break and release vacuolated hormogonia. To further confirm the isolate's identity, molecular analysis using 16S rRNA gene was performed. The sequence showed only 94% similarity to Leptolyngbya badia and less than 92% to other leptolyngbya. The phylogenetic analyses showed the coclustering of this strain with other Leptolyngbya strain. The fatty acid composition, used as a chemotaxonomic marker, revealed the presence of a considerable amount of polyunsaturated acids. Nevertheless, saturated fatty acids represented the highest proportion of the total fatty. Surprisingly, fatty acids of relatively limited occurrence within oscillatorian cyanobacteria such as saturated myristic fatty acid and polyunsaturated fatty acid C16:3 were found.

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Genes Encoding Proteins Homologous to Halobacterial Gvps N, J, K, F & L are Located Downstream ofgvpCin the CyanobacteriumAnabaena flos-aquae

Cited by 25 publications

References 30 publications

Analysis of tryptic digests indicates regions of GvpC that bind to gas vesicles of Anabaena flos-aquae

Analysis of tryptic digests indicates regions of GvpC that bind to gas vesicles of Anabaena flos-aquae

Elucidation of the gas vesicle gene clusters in cyanobacteria of the genusArthrospira(Oscillatoriales, Cyanophyta) and correlation with ITS phylogeny

Microscopic, molecular, and biochemical investigations to characterize a benthic cyanoprokaryote Leptolyngbya strainfrom Egypt

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