Generic simple enzyme immunoassay approach to avert small molecule immobilization problems on solid phasesApplication to the determination of tobramycin in serum

Darwish, Ibrahim A.; Mahmoud, Ashraf M.; Al-Majed, Abdulhakeem A.

doi:10.1016/j.talanta.2007.01.036

Cited by 13 publications

(2 citation statements)

References 23 publications

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“…This is the case of tobramycin determination in serum. 20 In addition, β-galactosidase is an enzyme which can be modified to exhibit allosteric responses. In particular, Escherichia coli β-galactosidase has been successfully tailored for the diagnosis of either foot-and-mouth disease 21 and human immunodeficiency virus (HIV) infection, 22,23 and conditions for assays in liquid and immobilized form have been completely established.…”

Section: Low-temperature Cofired Ceramics (Ltcc) Technology Is a Vers...mentioning

confidence: 99%

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Ceramic Microsystem Incorporating a Microreactor with Immobilized Biocatalyst for Enzymatic Spectrophotometric Assays

et al. 2009

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Low-temperature cofired ceramics (LTCC) technology is a versatile fabrication technique used to construct microflow systems. It permits the integration of several unitary operations (pretreatment, separation, (bio)chemical reaction, and detection stage) of an analytical process in a modular or monolithic way. Moreover, because of its compatibility with biological material, LTCC is adequate for analytical applications based on enzymatic reactions. Here we present the design, construction, and evaluation of a LTCC microfluidic system that integrates a microreactor (internal volume, 24.28 microL) with an immobilized beta-galactosidase from Escherichia coli (0.479 activity units) and an optical flow cell to measure the product of the enzymatic reaction. The enzyme was immobilized on a glyoxal-agarose support, maintaining its activity along the time of the study. As a proof of concept, the LTCC-beta-galactosidase system was tested by measuring the conversion of ortho-nitrophenyl beta-D-galactopyranoside, the substrate usually employed for activity determinations. Once packed in a monolithically integrated microcolumn, the miniaturized flow system was characterized, the operational conditions optimized (flow rate and injection volume), and its performance successfully evaluated by determining the beta-galactosidase substrate concentration at the millimolar level.

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Section: Low-temperature Cofired Ceramics (Ltcc) Technology Is a Vers...mentioning

confidence: 99%

“…On the other hand, the determination of its enzymatic activity has been also proposed as an indirect measure related to an analyte concentration. This is the case of tobramycin determination in serum …”

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confidence: 99%