One-step homogeneous non-stripping chemiluminescence metal immunoassay based on catalytic activity of gold nanoparticles

Qi, Yingying; Xiu, Fu-Rong; Li, Baoxin

doi:10.1016/j.ab.2013.12.007

Cited by 20 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar effect was observed for luminol-H 2 O 2 - Cit-AuNPs. Based on previous reports 41 44 45 46 47 48 we suggested that the enhancement effect of low concentrations of Cys on luminol–AgNO 3 -AuNPs reaction may be due to the change of the gold nanoparticles surface-charge density. It is obvious that the negative charge density of AuNPs surface decreases after aggregation which occurs in the presence of Cys.…”

Section: Resultsmentioning

confidence: 66%

Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

Shahrajabian

Hormozi-Nezhad

2016

Sci Rep

View full text Add to dashboard Cite

Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma.

show abstract

Section: Resultsmentioning

confidence: 66%

Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

Shahrajabian

Hormozi-Nezhad

2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Compared with Figure b, TEM image (Figure c) demonstrated that specific binding between anti-hIgG and hIgG led to the aggregation of MF-GNPs and a subsequent decrease in CL intensity. It was reported that aggregated GNPs induced by immunoreaction showed the enhanced catalytic effect on luminol CL reactions when luminol was in a solution because of low activation energy for electron transfer, proper surface area, and high electron density and oscillation effect in the conduction bands . Other than the previously reported work, in this case, CL reagent ABEI was directly bonded onto GNPs by the Au–N covalent interaction.…”

Section: Resultsmentioning

confidence: 79%

“…It was reported that aggregated GNPs induced by immunoreaction showed the enhanced catalytic effect on luminol CL reactions when luminol was in a solution because of low activation energy for electron transfer, proper surface area, and high electron density and oscillation effect in the conduction bands. 18 Other than the previously reported work, in this case, CL reagent ABEI was directly bonded onto GNPs by the Au−N covalent interaction. The intra-and interparticle quenching effects of GNPs on the fluorescence of the oxidation products of ABEI molecules on their surface were observed, 19 which were relatively small when CL molecules were in a solution.…”

Section: Resultsmentioning

confidence: 99%

Assembly of Multifunctionalized Gold Nanoparticles with Chemiluminescent, Catalytic, and Immune Activity for Label-Free Immunoassays

Huang

Gao

Cui

2018

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

In this study, we report a universal label-free immunoassay to detect antigen based on multifunctionalized gold nanoparticles (MF-GNPs), which were obtained by successive assembly of N-aminobutyl- N-ethylisoluminol functionalized gold nanoparticles (ABEI-GNPs) with antibody, bovine serum albumin (BSA) and Co. MF-GNPs exhibited excellent chemiluminescent (CL), catalytic and immune activity. It was demonstrated that the CL signal of MF-GNPs decreased in the presence of antigens via antigen-antibody specific binding using human immunoglobulin G (hIgG) and corresponding antibody goat anti-human IgG (anti-hIgG) as a model system, due to that immunoreaction led to the aggregation of GNPs. According to the decreased CL intensity, hIgG could be determined in the range of 1.0 fM to 1.0 nM with a low detection limit of 0.13 fM. Furthermore, this CL strategy was also confirmed to be a general one by replacing hIgG with heart-type fatty acid-binding protein (H-FABP), which is a biomarker of early acute myocardial infarction (AMI). The CL strategy could be employed to detect H-FABP ranging from 10.0 fM to 10.0 nM, and the detection limit is 7.8 fM. The CL strategy also showed good selectivity. It might be extended to detect other antigens if their corresponding antibodies are available.

show abstract

“…Subsequently, the author reported a similar CL sensor for thrombin detection using aptamer-mediated AuNP aggregation or dispersion . Combined with traditional immunoreaction recognition-induced cross-linking aggregation, a no-wash CL biosensor using AuNPs as catalysts was proposed for protein biomarker detection. , No-wash CL immunosensor is an excellent analytical technique of CL combined with conventional immunoassay, which has been extensively applied for the analysis of protein markers. The first demonstration of no-wash CL immunosensor was offered by Tatsu and co-workers .…”

Section: Chemiluminescence Biosensorsmentioning

confidence: 99%

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

et al. 2017

View full text Add to dashboard Cite

In vitro biosensors have been an integral component for early diagnosis of cancer in the clinic. Among them, no-wash biosensors, which only depend on the simple mixing of the signal generating probes and the sample solution without additional washing and separation steps, have been found to be particularly attractive. The outstanding advantages of facile, convenient, and rapid response of no-wash biosensors are especially suitable for point-of-care testing (POCT). One fast-growing field of no-wash biosensor design involves the usage of nanomaterials as signal amplification carriers or direct signal generating elements. The analytical capacity of no-wash biosensors with respect to sensitivity or limit of detection, specificity, stability, and multiplexing detection capacity is largely improved because of their large surface area, excellent optical, electrical, catalytic, and magnetic properties. This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells. Representative examples are selected to demonstrate the proof-of-concept with promising applications for in vitro diagnostics of cancer. Finally, a brief discussion of common unresolved issues and a perspective outlook on the field are provided.

show abstract

One-step homogeneous non-stripping chemiluminescence metal immunoassay based on catalytic activity of gold nanoparticles

Cited by 20 publications

References 39 publications

Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

Assembly of Multifunctionalized Gold Nanoparticles with Chemiluminescent, Catalytic, and Immune Activity for Label-Free Immunoassays

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

Contact Info

Product

Resources

About