Generation of stably expressing IRF5 spliced isoform in Jurkat cells

Abstract

Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein function. Here, we describe step by step optimized protocols for generating clonal stably expressing using crude lentiviral supernatant in Jurkat cells. Although the protocol is for general use, we will detail how to create stable cell lines based on Jurkat cells expressing IRF5 spliced isoform. These protocols will be broadly useful for researchers seeking to apply overexpression by viral transduction and generation of stable clone to study gene function in mammalian cells.

Therapeutic Targeting of MOAP-1 in Cancer: A Systematic Review of Current Approaches and Future Directions

“…The unprecedented COVID-19 pandemic has brought such a new challenge to biomedical scientists to push the frontier of biomedical research to innovate new molecules or pathways that could cut down the test-tube to market timeline [12][13][14] . This has also impacted cancer drug discovery by moving towards the use of in-silico modeling to elucidate the mechanistic pathway of newly found small molecules or proteins [15,16] that have good potential as vaccines and cancer therapeutics [17,18] .…”