Generation of PGS/PCL Blend Nanofibrous Scaffolds Mimicking Corneal Stroma Structure

Abstract

Morphological studies of aligned nanofibers scaffolds made of poly(glycerol sebacate)(PGS)/poly(ϵ‐caprolactone)(PCL) blends for application as corneal tissue scaffolds are presented. Parallel conductive bars are used as ground electrode to generate unidirectional nanofibers. Scaffolds have fibers diameter in the range of 550–300 nm. The structural stability and wettability, especially the in vitro degradation rate, of the electrospun scaffolds can be controlled by regulating the blended ratio of the polymers. The results indicate that PGS/PCL nanofiber scaffolds can be considered as ideal candidates for corneal tissue engineering scaffolds. The particular motivation for this work is to engineer a tissue‐like construct that will mimic the stromal tissue of the cornea.

Design and fabrication of poly (glycerol sebacate)‐based fibers for neural tissue engineering: Synthesis, electrospinning, and characterization

“…The chemical groups of the pPGS, crosslinked PVA‐PGS (60:40, 50:50, and 40:60), and PVA fibers were assessed using the FTIR spectroscopy as shown in Figure and 5. As described earlier, several pPGS polymer characteristic bands are observed in 1181 (CO stretching asymmetric), 1736 (CO carbonyl stretching), 2854 and 2926 (CH 2 stretching asymmetric), and 3100 to 3650 cm −1 (OH stretching) (Figure A) . The critical infrared bands of crosslinked PVA fibers are located in 867 (CH out of plane bending), 1122 (CO stretching), 1410 to 1510 (COH stretching), 1710 (CO stretching), 2730 and 2860 (CH 2 and CH 3 stretching), and 3200 to 3650 cm −1 (OH stretching) (Figure B) .…”

“…It is found that the water uptake values decrease with increasing PGS ratios. This behavior is ascribed to the low intrinsic water uptake of PGS reducing water uptake ratio . Therefore, the water contact angle decreases by increasing PGS ratio and subsequently reduces structural deformation that is a key requirement for neural tissue engineering …”

Poly(glycerol sebacate) biomaterial: synthesis and biomedical applications

“…The presence of these contacts is essential to establish the perception of vision in a subject. 52 Further biocompatibility tests of the material was carried out in vitro by the MTT assay on days 3, 5, and 7 to test the cell viability of HCECs. 51 The photoreceptor layers attached on the RGD-surface modified PGS membranes facilitate the handling of the entire entity as a free standing film.…”

Neue Möglichkeiten der Augenoberflächenrekonstruktion

“…Es verfügt über eine sehr gute Verträglichkeit in der Wechselwirkung mit Zellen [48,49]. Durch Optimierung der Elastomerherstellung im Elektrospinningverfahren konnte eine Degradierung erreicht werden, die jener einer humanen Amnionmembran sehr nahe kommt ( [50,51], . Abb.…”

“…Dieser Zusammenhang konnte nicht nur hinsichtlich kornealer Zellen, sondern auch hinsichtlich Muskelzellen, limbaler Epithelzellen und Fibroblasten demonstriert werden [13,52,53,54]. So wurde gezeigt, dass das Wachstumsverhalten der kornealen Zellen sowohl von der Anordnung als auch von der Dicke der Nanofibrillen anhängig ist [50,51]. Durch optimierte Spinningprozesse ist es möglich, eine parallele Ausrichtung der Nanofibrillen zu erreichen.…”