Generation of Naive-Like Porcine-Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development

Fujishiro, Shuh-hei; Nakano, K.; Mizukami, Yuya; Azami, Takuya; Arai, Yoshikazu; Matsunari, Hitomi; Ishino, Rikiya; Nishimura, Takashi; Watanabe, Masaru; Abe, Tomoyuki; Furukawa, Yutaka; Umeyama, Kazuhiro; Yamanaka, Shinya; Ema, Masatsugu; Nagashima, Hiroshi; Hanazono, Yutaka

doi:10.1089/scd.2012.0173

Cited by 116 publications

(144 citation statements)

References 62 publications

(72 reference statements)

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“…Our next objective is the derivation of rabbit naive pluripotent stem cells, which could be controlled via their hOCT3/4 expression by doxycycline and confirmed by their germ line transmission in chimeric rabbits, and then assessment of the in vitro differentiation capacity of these cells. Recently, new methods for establishing high quality pluripotent stem cells in a naive-like state have been developed in the human and pig (38,54,55), so naivelike conversion could be standardized in several research areas. Pluripotent stem cells are initially generated in rabbits in a primed state and can then be converted to the naive-like state.…”

Section: Discussionmentioning

confidence: 99%

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Honda

Hatori

Hirose

et al. 2013

Journal of Biological Chemistry

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Background: The quality of induced pluripotent stem (iPS) cells might be inherently worse than embryonic stem cells. Results: Although the differentiation capacity of iPS cells is limited, it can be enhanced. Conclusion: Improving their level of pluripotency alleviated the limited capacity of iPS cells. Significance: This report offers an effective strategy for the development of iPS cell-based research.

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Section: Discussionmentioning

confidence: 99%

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Honda

Hatori

Hirose

et al. 2013

Journal of Biological Chemistry

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“…Gene targeting by homologous recombination is extremely inefficient in porcine somatic cells 3 , and porcine embryonic stem cells (ES) have not yet been established 15 . Recently, several groups described the derivation of porcine induced pluripotent cells (iPS) [16][17][18][19][20] , however, only low chimerism has been found after blastocyst complementation experiments 21,22 . The employment of porcine iPS cells in the SCNT method resulted in extremely low rates of born piglets 23 .…”

Section: Introductionmentioning

confidence: 99%

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

et al. 2014

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2The pig has emerged as an important large animal model in biomedical and pharmaceutical research.We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beauty transposon system. The protocol is based on co-injection of a plasmid encoding the SB100X hyperactive transposase together with a second plasmid carrying a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes. The transposase mediates excision of the transgene cassette from the plasmid vector and its permanent insertion into the genome to produce stable transgenic animals. This method compares favorably in terms of both efficiency and reliable transgene expression to classic pronuclear microinjection or somatic cell nuclear transfer, and offers comparable efficacies to lentiviral approaches, without limitations on vector design, issues of transgene silencing as well as the toxicity and biosafety concerns of working with viral vectors. Microinjection of the vectors into zygotes and transfer of the embryos to recipient animals can be performed in one day; generation of germline-transgenic lines by using this protocol takes approximately one year.

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“…Porcine iPSCs have been produced in many laboratories using various induction methods and show pluripotency to some degree [59,93,96,97] . Porcine iPSCs could pass the test of germline chimera production at the molecular genotyping levels using PCR [42,98] , but stable chimerism remains to be determined.…”

Section: Progress On Inducing Pluripotent Stem Cells Of Pig and Othermentioning

confidence: 99%

“…Therefore, X-chromosome activation is considered to be a key indicator that a pluripotent cell is in a naive or primed state [155] . For porcine iPSCs, three cell lines generated by the expression of transcription factors OCT4, KLF4 and C-MYC showed naive-like iPSC properties with an activated X-chromosome, as well as high embryonic chimera incorporation efficiency [97,126,130] . In addition, Haraguchi and colleagues successfully established a unique cell line derived from ICM of porcine embryo that exhibited LIF-dependency but not bFGFdependency, and could be considered as naive-state cells as mouse ESCs; however, the X-chromosome status in this cell line was XaXi [45] .…”

Section: X-chromosome Activationmentioning

confidence: 99%

Porcine pluripotent stem cells: progress, challenges and prospects

Han¹,

Miao²,

Hua³

et al. 2019

Front. Agr. Sci. Eng.

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Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.

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Generation of Naive-Like Porcine-Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development

Cited by 116 publications

References 62 publications

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

Porcine pluripotent stem cells: progress, challenges and prospects

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