Porcine pluripotent stem cells: progress, challenges and prospects

Han, Jianyong; Miao, Yi‐Liang; Hua, Jinlian; Li, Yan; Zhang, Xue; Zhou, Jilong; Li, Na; Zhang, Ying; Zhang, Jinying; Z, Liu

doi:10.15302/j-fase-2018233

Cited by 3 publications

(2 citation statements)

References 180 publications

(220 reference statements)

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“…These species-specific differences in morphology and timing during early embryogenesis have influenced efforts to determine how and when pPSCs can be stabilized in vitro . Many review articles have elegantly summarized these past efforts in detail ( Talbot and Blomberg, 2008 ; Blomberg and Telugu, 2012 ; Gandolfi et al, 2012 ; Malaver-Ortega et al, 2012 ; Koh and Piedrahita, 2014 ; Gonçalves, Ambrósio, and Piedrahita, 2014 ; Ezashi, Yuan, and Roberts, 2016 ; Han et al, 2019 ; Zhang et al, 2022 ), much of which could not be included in this mini review due to limitations in scope.…”

Section: Early Challenges In Translating Mouse and Primate Psc Deriva...mentioning

confidence: 99%

The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances

Neira,

Conrad,

Rusteika

et al. 2024

Front. Cell Dev. Biol.

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Pigs (Sus scrofa) are widely acknowledged as an important large mammalian animal model due to their similarity to human physiology, genetics, and immunology. Leveraging the full potential of this model presents significant opportunities for major advancements in the fields of comparative biology, disease modeling, and regenerative medicine. Thus, the derivation of pluripotent stem cells from this species can offer new tools for disease modeling and serve as a stepping stone to test future autologous or allogeneic cell-based therapies. Over the past few decades, great progress has been made in establishing porcine pluripotent stem cells (pPSCs), including embryonic stem cells (pESCs) derived from pre- and peri-implantation embryos, and porcine induced pluripotent stem cells (piPSCs) using a variety of cellular reprogramming strategies. However, the stabilization of pPSCs was not as straightforward as directly applying the culture conditions developed and optimized for murine or primate PSCs. Therefore, it has historically been challenging to establish stable pPSC lines that could pass stringent pluripotency tests. Here, we review recent advances in the establishment of stable porcine PSCs. We focus on the evolving derivation methods that eventually led to the establishment of pESCs and transgene-free piPSCs, as well as current challenges and opportunities in this rapidly advancing field.

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Section: Early Challenges In Translating Mouse and Primate Psc Deriva...mentioning

confidence: 99%

The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances

Neira,

Conrad,

Rusteika

et al. 2024

Front. Cell Dev. Biol.

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“…Hearts from genetically modified pigs could be transplanted into baboons and that can be kept alive 155 . However, until now, the true pig embryonic stem cells have not been established 156 …”

Section: Prospectmentioning

confidence: 99%

Super enhancers—Functional cores under the 3D genome

Zhang

Zhou

et al. 2020

Cell Proliferation

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Cell fate is precisely regulated in the developmental process and human disease. Since the discovery of Mendelian, gene regulation models are continuously developing. 1-4 In the beginning, the transcriptional models were linear. 5-8 In the 1980s, the first enhancer was identified which can increase gene expression by 200-fold. 9 With technological development, the study of chromatin is expanded into three dimensions (3D). Distal enhanced elements transmit the activation signal to the promoter by chromosome folding. 10,11 These advances further explained the accuracy of gene expression regulation, enriching theories of transcriptional models. In 2013, Young and colleagues came up with the concept of the super enhancer (SE), as the region in the embryonic stem (ES) cells where Oct4, Sox2 and Nanog co-bind, 12,13 with a huge region of the genome. 14-16 Later, more and more biological macromolecules were found in the formation of SEs, and more biological functions were found to be controlled by them. 17-21 Based on the

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