Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1

Abstract

The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1, which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs specific to ribosomal protein S6 kinase 1 have been generated. Specificity of mAbs has been confirmed by Western blot and immunoprecipitation technique. Conclusions. The obtained antibodies are suitable for elucidating signal transduction pathways involving S6K1

PI3K/mTOR/S6K signaling pathway – new players and new functional links

“…For analysis of S6K1 and S6K2 expression level and kinases subcellular localization we generated a set of monoclonal and polyclonal antibodies specific for Nand C-terminal parts of S6K1/2 [11][12][13]. According to our data S6K1 and S6K2 have a tissue specific expression profile with the highest level in brain and testis respectively [14].…”