PI3K/mTOR/S6K signaling pathway – new players and new functional links

Filonenko, Valeriy

doi:10.7124/bc.00081a

Cited by 10 publications

(8 citation statements)

References 51 publications

(60 reference statements)

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“…Serine 2481 localizes at the regulatory domain of the kinase and could be involved in the modulation of its activity [8,9]. In mammalian cells mTOR exists as a core enzyme of two different complexes: mTORC1 and mTORC2 [13,14,15]. Earlier studies showed that specific mTOR inhibitor rapamycin treatment did not affect Serine 2481 phosphorilation.…”

Section: Discussionmentioning

confidence: 99%

Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase

et al. 2016

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Aim.To study the subcellular localization of phospho-mTOR (Ser2481) in cultured cells of human cancer cell lines. Methods. Immunofluorescent analysis of phospho-mTOR (Ser2481) subcellular localization in cultured MCF-7 (human breast adenocarcinoma) and HepG2 (human liver carcinoma) cells. Results. For the first time phospho-mTOR (Ser2481) was detected as bright spots situated in the equatorial region of chromosomes and co-localized with condensed chromosomes during metaphase. Conclusions. In this study, we describe for the first time the co-localization of phospho-mTOR (Ser2481) and chromosomes of the metaphase plate in the MCF-7 and HepG2 cells. Our data support the hypothesis that phospho-mTOR (Ser2481) could act as a CPP (chromosomal passenger protein)-like kinase, which takes part in the regulation of mitosis progression. K e y w o r d s: mTOR, phospho-mTOR (Ser2481), mitosis, mTOR phosphorylation, metaphase.

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Section: Discussionmentioning

confidence: 99%

Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase

et al. 2016

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“…To compare the proposed approach with the routine measurement of the distance between the layers of cells, we performed an analysis of the MCF-7 and HeLa cells moti- mTOR is a central connecting link in the signaling network that regulates the basic cellular function such as translation and transcription, and therefore, has an important impact on the regulation of major cellular function including cell migration [15]. But the exact molecular mechanisms by which mTOR regulates the cell migration remain to be determined [16].…”

Section: Application Of the Both Methods For The Evaluation Of Migratmentioning

confidence: 99%

Optimization of cell motility evaluation in scratch assay

et al. 2014

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A scratch test is one of the most popular methods of classical cell migration assay in a monolayer culture. At the same time, the scratch assay has some disadvantages that can be easily corrected. Aim. Optimization the existent scratch assay on the base of detection of scratch wound surface area and the length of the field of observation which is more objective and less time consuming. Methods. Scratch assay. Results. The modification of scratch assay enables to perform measurement more accurately and rapidly. This approach is more simple and eliminates the main disadvantages of the classical method. Conclusions. The procedure of scratch wound width measurement calculated on the base of detection of cell free area and the length of the field of observation is more effective than the classical wound healing assay. It will be useful for the estimation of cell migration dynamics in monolayer culture for a wide range of live cell based experiments.

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“…Besides the canonical function of the mentioned mTOR targets, they are involved in the regulation of cell viability, migration, cytoskeleton remodeling etc. [17][18][19].…”

Section: Components and Substrates Of Mtor Complexmentioning

confidence: 99%

mTOR signaling and its involvement in the regulation of cell movements through remodeling the cytoskeleton architecture

2015

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mTOR kinase is one of the basic links at the crossroad of several signal transduction pathways. De re gulated mTOR kinase signaling accompanies the progress of cancer, diabetes, neurodegenerative disorders and aging. Implication of mTOR inhibitor rapamycin decreases migration and invasion of malignant cells, and metastasis formation. However, a precise mechanism of the regulation of cellular locomotion by mTOR kinase is not fully understood. This article focuses on the recent fi ndings that demonstrated a possible role of mTOR kinase in the regulation of cytoskeleton remodeling and cell migration properties. Detailed studies on this non-canonical mTOR function will extend our knowledge about cell migration and metastasis formation and might improve anti-cancer therapeutic approaches.K e y w o r d s: mTOR signaling, rapamycin, cytoskeleton remodeling, intermediate fi laments, microtubules, cancer metastasis.

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PI3K/mTOR/S6K signaling pathway – new players and new functional links

Cited by 10 publications

References 51 publications

Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase

Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase

Optimization of cell motility evaluation in scratch assay

mTOR signaling and its involvement in the regulation of cell movements through remodeling the cytoskeleton architecture

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