Generation of marker free salt tolerant transgenic plants of Arabidopsis thaliana using the gly I gene and cre gene under inducible promoters

Roy, Suchandra Deb; Saxena, Mukesh; Bhomkar, Prasanna; Pooggin, Mikhail M.; Höhn, Thomas; Bhalla‐Sarin, Neera

doi:10.1007/s11240-008-9402-0

Cited by 37 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The deduced TcGLX1 polypeptide was compared with Gly I and TaGly I from B. juncea and T. aestivum, respectively, which have been used to increase Zn tolerance (Singla-Pareek et al 2006;Lin et al 2010) and Zn accumulation (Singla-Pareek et al 2006) in tobacco. While the deduced polypeptide sequence of T. caerulescens GLX1 shows high identity with the polypeptide in B. rapa and ATGLX1 in A. thaliana (90-92%), the identity with T. aestivum TaGly I is 74% (Lin et al 2010) and with B. juncea Gly I only 22-23% (Veena et al 1999;SinglaPareek et al 2003SinglaPareek et al , 2006Bhomkar et al 2008;Roy et al 2008). The Gly I polypeptide of B. juncea is short, the deduced molecular mass being ca.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the glyoxalase 1 gene TcGLX1 in the metal hyperaccumulator plant Thlaspi caerulescens

Tuomainen

Ahonen

Kärenlampi

et al. 2011

Planta

View full text Add to dashboard Cite

Stress tolerance is currently one of the major research topics in plant biology because of the challenges posed by changing climate and increasing demand to grow crop plants in marginal soils. Increased Zn tolerance and accumulation has been reported in tobacco expressing the glyoxalase 1-encoding gene from Brassica juncea. Previous studies in our laboratory showed some Zn tolerance-correlated differences in the levels of glyoxalase 1-like protein among accessions of Zn hyperaccumulator Thlaspi caerulescens. We have now isolated the corresponding gene (named here TcGLX1), including ca. 570 bp of core and proximal promoter region. The predicted protein contains three glyoxalase 1 motifs and several putative sites for post-translational modification. In silico analysis predicted a number of cis-acting elements related to stress. The expression of TcGLX1 was not responsive to Zn. There was no correlation between the levels of TcGLX1 expression and the degrees of Zn tolerance or accumulation among T. caerulescens accessions nor was there co-segregation of TcGLX1 expression with Zn tolerance or Zn accumulation among F3 lines derived from crosses between plants from accessions with contrasting phenotypes for these properties. No phenotype was observed in an A. thaliana T-DNA insertion line for the closest A. thaliana homolog of TcGLX1, ATGLX1. These results suggest that glyoxalase 1 or at least the particular isoform studied here is not a major determinant of Zn tolerance in the Zn hyperaccumulator plant T. caerulescens. In addition, ATGLX1 is not essential for normal Zn tolerance in the non-tolerant, non-accumulator plant A. thaliana. Possible explanations for the apparent discrepancy between this and previous studies are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the glyoxalase 1 gene TcGLX1 in the metal hyperaccumulator plant Thlaspi caerulescens

Tuomainen

Ahonen

Kärenlampi

et al. 2011

Planta

View full text Add to dashboard Cite

show abstract

“…A number of genes orthologous to those we have isolated from S. stapfianus have been successfully utilised to enhance drought or salt or cold tolerance in model plants or important crop species. These include glyoxylase1, catalase, cysteine protease, several group 2 and group 3 LEAs, aquaporins, a myb transcription factor and the translation initiation factor eIF1 (Mohamed et al 2003;Villalobos et al 2004;Sheokand et al 2005;Brini et al 2007;Cui et al 2008;Diédhiou et al 2008;Roy et al 2008;Wang et al 2009a). Approaches to using genes associated with desiccation tolerance for improving drought tolerance in crop species have recently been reviewed by Toldi et al (2009) and Moore et al (2008b).…”

Section: Isolation Of Desiccation-tolerance Specific Genesmentioning

confidence: 99%

The resurrection plant Sporobolus stapfianus: An unlikely model for engineering enhanced plant biomass?

et al. 2010

View full text Add to dashboard Cite

The resurrection grass Sporobolus stapfianus Gandoger can rapidly recover from extended periods of time in the desiccated state (water potential equilibrated to 2% relative humidity) (Gaff and Ellis, Bothalia 11:305-308 1974; Gaff and Loveys, Transactions of the Malaysian Society of Plant Physiology 3:286-287 1993). Physiological studies have been conducted in S. stapfianus to investigate the responses utilised by these desiccationtolerant plants to cope with severe water-deficit. In a number of instances, more recent gene expression analyses in S. stapfianus have shed light on the molecular and cellular mechanisms mediating these responses. S. stapfianus is a versatile research tool for investigating desiccation-tolerance in vegetative grass tissue, with several useful characteristics for differentiating desiccation-tolerance adaptive genes from the many dehydration-responsive genes present in plants. A number of genes orthologous to those isolated from dehydrating S. stapfianus have been successfully used to enhance drought and salt tolerance in model plants as well as important crop species. In addition to the ability to desiccate and rehydrate successfully, the survival of resurrection plants in regions experiencing short sporadic rainfall events may depend substantially on the ability to tightly down-regulate cell division and cell wall loosening activities with decreasing water availability and then grow rapidly after rainfall while water is plentiful. Hence, an analysis of gene transcripts present in the desiccated tissue of resurrection plants may reveal important growth-related genes. Recent findings support the proposition that, as well as being a versatile model for devising strategies for protecting plants from water-loss, resurrection plants may be a very useful tool for pinpointing genes to target for enhancing growth rate and biomass production.

show abstract

“…Although previous studies have reported the best performance of constitutive promoters in regulating the glyoxalase pathway gene(s) during abiotic stress conditions, data regarding the performance of the same during unstressed conditions are missing. A few studies have been conducted using stress-inducible ''responsive to desiccation'' (rd29A) gene promoter to regulate the expression of gly I gene in transgenic plants (Rajwanshi et al 2007;Roy et al 2008). Previous studies have shown that rd29A and rd29B genes are induced under high temperature, high salt, and drought or with exogenous abscisic acid (ABA) treatment due to the presence of drought-responsive element (DRE) and ABA-responsive element (ABRE) Shinozaki 1993, 1994;Bihmidine et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Stress-inducible overexpression of glyoxalase I is preferable to its constitutive overexpression for abiotic stress tolerance in transgenic Brassica juncea

et al. 2016

View full text Add to dashboard Cite

The glyoxalase system catalyzes the conversion of cytotoxic methylglyoxal to D-lactate via the intermediate S-D-lactoylglutathione. It comprises two enzymes, Glyoxalase I (Gly I) and Glyoxalase II (Gly II), and reduced glutathione which acts as a cofactor by anchoring the substrates in the active sites of the two enzymes. The overexpression of both Gly I and Gly II, either alone or in combination, has earlier been reported to confer tolerance to multiple abiotic stresses. In the present study, we sought to evaluate the consequences of constitutive and stress-induced overexpression of Gly I on the performance and productivity of plants. Towards this end, several Gly I transgenic Brassica juncea lines (designated as R and S lines) were generated in which the glyoxalase I (gly I) gene was expressed under the control of either a stress-inducible rd29A promoter or a constitutive CaMV 35S promoter. Both the R and S lines showed enhanced tolerance to salinity, heavy metal, and drought stress when compared to untransformed control plants. However, the S lines showed yield penalty under non-stress conditions while no such negative effect was observed in the R lines. Our results indicate that the overexpression of the gly I gene under the control of stress-inducible rd29A promoter is a better option for improving salt, drought and heavy metal stress tolerance in transgenic plants.Keywords Brassica juncea Á Glyoxalase I Á CaMV 35S promoter Á rd29A promoter Á Abiotic stress Á Transgenic Electronic supplementary material The online version of this article (

show abstract

Generation of marker free salt tolerant transgenic plants of Arabidopsis thaliana using the gly I gene and cre gene under inducible promoters

Cited by 37 publications

References 35 publications

Characterization of the glyoxalase 1 gene TcGLX1 in the metal hyperaccumulator plant Thlaspi caerulescens

Characterization of the glyoxalase 1 gene TcGLX1 in the metal hyperaccumulator plant Thlaspi caerulescens

The resurrection plant Sporobolus stapfianus: An unlikely model for engineering enhanced plant biomass?

Stress-inducible overexpression of glyoxalase I is preferable to its constitutive overexpression for abiotic stress tolerance in transgenic Brassica juncea

Contact Info

Product

Resources

About