Generation of insulin-producing cells from rat mesenchymal stem cells using an aminopyrrole derivative XW4.4

Ouyang, Jingping; Huang, Wei; Yu, Wei; Xiong, Wei; Mula, Ramanjaneya V.R.; Zou, Haifeng; Yu, Yongping

doi:10.1016/j.cbi.2013.11.007

Cited by 14 publications

(3 citation statements)

References 37 publications

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“…Many protocols have been developed to differentiate MSCs into functional insulin-producing β-cells by exposing the cells to chemical and biological factors or by genetic manipulation introducing the PDX1 gene, which is a master regulator in pancreas organogenesis [ 232 , 437 , 440 , 441 , 442 , 443 , 444 , 445 , 446 , 447 , 448 , 449 , 450 , 451 , 452 , 453 , 454 , 455 , 456 , 457 , 458 , 459 ]. A common dominator for the different differentiation protocols is the sequential exposure of MSCs to different combinations of growth factors (e.g., EGF, bFGF, betacellulin, activin A, HGF, extendin-4, insulin) chemical compounds (e.g., nicotinamide), and B27 supplement (containing among others insulin, biotin, vitamin E, Vitamin A, selenium, putrescine, transferrin, catalase, superoxide dismutase, triodo-L-thyronine, linoleic and linolenic acids) for different time periods [ 441 , 442 ].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

A Supportive Role of Mesenchymal Stem Cells on Insulin-Producing Langerhans Islets with a Specific Emphasis on The Secretome

Sionov,

Ahdut-HaCohen

2023

Biomedicines

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Type 1 Diabetes (T1D) is a chronic autoimmune disease characterized by a gradual destruction of insulin-producing β-cells in the endocrine pancreas due to innate and specific immune responses, leading to impaired glucose homeostasis. T1D patients usually require regular insulin injections after meals to maintain normal serum glucose levels. In severe cases, pancreas or Langerhans islet transplantation can assist in reaching a sufficient β-mass to normalize glucose homeostasis. The latter procedure is limited because of low donor availability, high islet loss, and immune rejection. There is still a need to develop new technologies to improve islet survival and implantation and to keep the islets functional. Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor cells with high plasticity that can support human pancreatic islet function both in vitro and in vivo and islet co-transplantation with MSCs is more effective than islet transplantation alone in attenuating diabetes progression. The beneficial effect of MSCs on islet function is due to a combined effect on angiogenesis, suppression of immune responses, and secretion of growth factors essential for islet survival and function. In this review, various aspects of MSCs related to islet function and diabetes are described.

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Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

A Supportive Role of Mesenchymal Stem Cells on Insulin-Producing Langerhans Islets with a Specific Emphasis on The Secretome

Sionov,

Ahdut-HaCohen

2023

Biomedicines

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“…Differentiation of P-ADSCs into insulin-producing cells P-ADSCs were differentiated into insulin-secreting cells using the methods described previously (Zhang et al 2011;Dave et al 2014;Ouyang et al 2014) with some modifications. The first step of the method was seeding P-ADSCs into a 100 mm dish (1 x10 6 cells/ dish) containing 2% FBS/DMEM (high glucose) supplemented with 1% non-essential amino acids (NEAA) and 0.5 mM b-mercaptoethanol (Sigma) for 2 days.…”

Section: Chondrogenic Differentiationmentioning

confidence: 99%

Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat

Chen

et al. 2017

Cytotechnology

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The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery. We used a homogenizer to mince fat and compared the results with those obtained from the traditional cut method involving a sterile scalpel and forceps. Our results showed that our method provides another stable and quality source of stem cells that could be used in cases with a large quantity of fat. Furthermore, we found that pregnancy adipose-derived stem cells (P-ADSCs) could be maintained in vitro for extended periods with a stable population doubling and low senescence levels. P-ADSCs could also differentiate in vitro into adipogenic, osteogenic, chondrogenic, and insulin-producing cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirates, adipose tissues obtained from pregnant women contain multipotent cells with better proliferation and showed great promise for use in both stem cell banking studies as well as in stem cell therapy.

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“…In a recent study, Ouyang et al induced rat BMMSCs to differentiate into IPCs with various growth factors and small molecules through 3-step protocols. They concluded that, in their comparative study, aminopyrrole derivative compound XW4.4 revealed the best activity among them and differentiated cells exhibited morphological and functional characteristics of IPCs and expression of pancreatic endocrine cell marker genes [ 111 ].…”

Section: Mscs Application In Experimental Studies and Differentiatmentioning

confidence: 99%

Mesenchymal Stem Cells: Rising Concerns over Their Application in Treatment of Type One Diabetes Mellitus

Hashemian

Kouhnavard

Nasli‐Esfahani

2015

Journal of Diabetes Research

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Type 1 diabetes mellitus (T1DM) is an autoimmune disorder that leads to beta cell destruction and lowered insulin production. In recent years, stem cell therapies have opened up new horizons to treatment of diabetes mellitus. Among all kinds of stem cells, mesenchymal stem cells (MSCs) have been shown to be an interesting therapeutic option based on their immunomodulatory properties and differentiation potentials confirmed in various experimental and clinical trial studies. In this review, we discuss MSCs differential potentials in differentiation into insulin-producing cells (IPCs) from various sources and also have an overview on currently understood mechanisms through which MSCs exhibit their immunomodulatory effects. Other important issues that are provided in this review, due to their importance in the field of cell therapy, are genetic manipulations (as a new biotechnological method), routes of transplantation, combination of MSCs with other cell types, frequency of transplantation, and special considerations regarding diabetic patients' autologous MSCs transplantation. At the end, utilization of biomaterials either as encapsulation tools or as scaffolds to prevent immune rejection, preparation of tridimensional vascularized microenvironment, and completed or ongoing clinical trials using MSCs are discussed. Despite all unresolved concerns about clinical applications of MSCs, this group of stem cells still remains a promising therapeutic modality for treatment of diabetes.

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Generation of insulin-producing cells from rat mesenchymal stem cells using an aminopyrrole derivative XW4.4

Cited by 14 publications

References 37 publications

A Supportive Role of Mesenchymal Stem Cells on Insulin-Producing Langerhans Islets with a Specific Emphasis on The Secretome

A Supportive Role of Mesenchymal Stem Cells on Insulin-Producing Langerhans Islets with a Specific Emphasis on The Secretome

Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat

Mesenchymal Stem Cells: Rising Concerns over Their Application in Treatment of Type One Diabetes Mellitus

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