Investigating the mincing method for isolation of adipose-derived stem cells from pregnant women fat

Abstract

The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery. We used a homogenizer to mince fat and compared the results with those obtained from the traditional cut method involving a sterile scalpel and forceps. Our results showed that our method provides another stable and quality source of stem cells that could be used in cases with a large quantity of fat. Furthermore, we found that pregnancy adipose-derived stem cells (P-ADSCs) could be maintained in vitro for extended periods with a stable population doubling and low senescence levels. P-ADSCs could also differentiate in vitro into adipogenic, osteogenic, chondrogenic, and insulin-producing cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirates, adipose tissues obtained from pregnant women contain multipotent cells with better proliferation and showed great promise for use in both stem cell banking studies as well as in stem cell therapy.

Isolation of Adipose Tissue–Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue–Derived Stem Cell Yield from Human Aspirated Fat

“…Mechanical disruption finely dissociates the tissue and, by increasing the surface area of the tissue, provides a more efficient enzymatic digestion, resulting in an increased MSC yield (Table ). The method is supported by a study investigating excised adipose tissue (Li et al., ), where the authors found an increase in SVF cell yield with prior tissue disruption for 15 sec before enzymatic digestion. Their study, however, reports specific yield of the heterogeneous SVF population and not a precise measurement of phenotypically characterized MSCs.…”

“…To optimize MSC extraction, different isolation techniques have been investigated (Domenis et al., ; Faustini et al., ), including enzymatic isolation, mechanical isolation, and automated systems. Only a single published study combines enzymatic and mechanical MSC isolation (Li et al., ), and these authors describe an increased yield of the stromal vascular fraction (SVF) from excised adipose tissue. However, excision of adipose tissue is a more complicated procedure than aspiration.…”

Nöroanastomoz Sonrası Stromal Vasküler Fraksiyonun Nörorejeneratif Etkisinin Histolojik ve Elektromiyografik Değerlendirilmesi

“…Subsequent adipose tissues were washed with Dulbecco's phosphate buff ered saline (D-PBS) separately performed in sterile petri dish. Adipose tissue samples was removed from D-PBS, placed into the 100 mm 2 petri dish and cut into the more small pieces (1-2 mm 2 ) for mincing process [26] . After this mincing process, 1.5 g of fat tissue + 1.5 mL of 0.08% isotonic content were added to 2 centrifuge tubes (15 mL volume).…”