Generation of induced pluripotent stem cell line (FMCPGHi001-A) from a 25-year-old Chinese Han healthy male donor

Wang, Tao; Tang, Wenjing; Zeng, Quan; Miao, Shuai; Pi, Chenghui; Li, Yingji; Yue, Wen; Yu, Shengyuan

doi:10.1016/j.scr.2022.102735

Cited by 3 publications

(1 citation statement)

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“…Ethical Approval All research that involved human cells and tissues was carried out in accordance with the principles of the Helsinki Declaration. hiPSC Culture and Generation of hiPSC-Derived Retinal Organoids As previously shown, the hiPSC line (FMCPGHi001-A) used in this study was validated using standardised methods [18] . The hiPSC line was grown in mTeSR1 media (StemCell Technologies, Vancouver, Canada) on Matrigel-coated (Corning Inc., Corning, NY, USA) plates and passaged at 80% confluence every 5-7d.…”

Section: Methodsmentioning

confidence: 99%

Influence of hypoxia on retinal progenitor and ganglion cells in human induced pluripotent stem cell-derived retinal organoids

Du,

Wang,

et al. 2023

Int J Ophthalmol

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AIM: To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell (hiPSC)-derived retinal organoids (ROs). METHODS: The hiPSC and a three-dimensional culture method were used for the experiments. Generated embryoid bodies (EBs) were randomly and equally divided into hypoxic and normoxic groups. Photographs of the EBs were taken on days 38, 45, and 52, and the corresponding volume of EBs was calculated. Simultaneously, samples were collected at these three timepoints, followed by fixation, sectioning, and immunofluorescence. RESULTS: The proportion of Ki67-positive proliferating cells increased steadily on day 38; this proliferation-promoting effect tended to increase tissue density rather than tissue volume. On days 45 and 52, the two groups had relatively similar ratios of Ki67-positive cells. Further immunofluorescence analysis showed that the ratio of SOX2-positive cells significantly increased within the neural retina on day 52 (P<0.05). In contrast, the percentage of PAX6- and CHX10-positive cells significantly decreased following hypoxia treatment at all three timepoints (P<0.01), except for CHX10 at day 45 (P>0.05). Moreover, the proportion of PAX6-/TUJ1+ cells within the neural retinas increased considerably (P<0.01, <0.05, <0.05 respectively). CONCLUSION: Low oxygen promotes stemness and proliferation of neural retinas, suggesting that hypoxic conditions can enlarge the retinal progenitor cell pool in hiPSC-derived ROs.

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Section: Methodsmentioning

confidence: 99%