Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor

Al-Shehri, Mohammad; Baadhaim, Moayad; Jamalalddin, Shereen; Aboalola, Doaa; Daghestani, Mustafa; Alzahrani, Hajar; Malibari, Dalal; Mubaraki, Mohammad; Aldubayan, Kholoud; Balwi, Mohammed Al; Alsayegh, Khaled

doi:10.1016/j.scr.2021.102548

Cited by 4 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to their lack of genetic mutations and genomic structural variation, including the absence of TCR/BCR gene recombination found in T cells, EPCs were selected for reprogramming [ 40 ]. We, therefore, established two DRVT-iPSC lines using a non-integrative and virus-free reprogramming approach as previously described [ 29 ]. Briefly, the episomal vectors encoding OCT4, SOX2, KLF4, L-MYC, LIN28A, dominant-negative form of TP53, and EBNA1 were delivered to EPCs by electroporation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

Alowaysi,

Al-Shehri,

Badkok

et al. 2023

Human Cell

Self Cite

View full text Add to dashboard Cite

The most prevalent form of epileptic encephalopathy is Dravet syndrome (DRVT), which is triggered by the pathogenic variant SCN1A in 80% of cases. iPSCs with different SCN1A mutations have been constructed by several groups to model DRVT syndrome. However, no studies involving DRVT-iPSCs with rare genetic variants have been conducted. Here, we established two DRVT-iPSC lines harboring a homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A gene. Therefore, the derivation of these iPSC lines provides a unique cellular platform to dissect the molecular mechanisms underlying the cellular dysfunctions consequent to CPLX1 and SCN9A mutations.

show abstract

Section: Resultsmentioning

confidence: 99%

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

Alowaysi,

Al-Shehri,

Badkok

et al. 2023

Human Cell

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to their lack of genetic mutations and genomic structural variation, including the absence of TCR/BCR gene recombination found in T-cells, EPCs were selected for reprogramming (Chou et al, 2011). We; therefore, established two DRVT-iPSC lines using a non-integrative and virus-free reprogramming approach as previously described [29]. Brie y, the episomal vectors encoding OCT4, SOX2, KLF4, L-MYC, LIN28A, dominant-negative form of TP53, and EBNA1 was delivered to EPCs by electroporation (Fig.…”

Section: The Generation Of Integration-free Drvt-ipsc Linesmentioning

confidence: 99%

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet Syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

Alowaysi,

Al-Shehri,

Badkok

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The most prevalent form of epileptic encephalopathy is Dravet Syndrome (DRVT), which is triggered by the pathogenic variant SCN1A in 80% of cases. iPSCs with different SCN1A mutations have been constructed by several groups to model DRVT syndrome. However, no studies involving DRVT-iPSCs with rare genetic variants have been conducted. Here, we established two DRVT-iPSC lines harboring a homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A gene. Therefore, the derivation of these iPSC lines provides a unique cellular platform to dissect the molecular mechanisms underlying the cellular dysfunctions consequent to CPLX1 and SCN9A mutations.

show abstract

iPSC-Derived Corneal Endothelial Cells

Zhou

2023

Human iPSC-derived Disease Models for Drug Discovery

View full text Add to dashboard Cite

Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor

Cited by 4 publications

References 1 publication

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet Syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A

iPSC-Derived Corneal Endothelial Cells

Contact Info

Product

Resources

About