Generation of human iPSCs from urine derived cells of a non-affected control subject

Afzal, Muhammad Bilal; Gartz, Melanie; Klyachko, Ekaterina; Khan, Sadiya S.; Shah, Sanjiv J.; Gupta, Sweta; Shapiro, Amy D.; Vaughan, Douglas E.; Strande, Jennifer L.

doi:10.1016/j.scr.2016.12.008

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…Briefly, cells are seeded into a 96 well plate at densities of 0.5 and 1 cell per well. After 14 days, colonies derived from single cells become visible, and can be expanded [78].…”

Section: Molecular and Functional Characterization Of Kidney Derived Cell Linesmentioning

confidence: 99%

Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Bondue

Arcolino

Veys

et al. 2021

Cells

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Epithelial cells exfoliated in human urine can include cells anywhere from the urinary tract and kidneys; however, podocytes and proximal tubular epithelial cells (PTECs) are by far the most relevant cell types for the study of genetic kidney diseases. When maintained in vitro, they have been proven extremely valuable for discovering disease mechanisms and for the development of new therapies. Furthermore, cultured patient cells can individually represent their human sources and their specific variants for personalized medicine studies, which are recently gaining much interest. In this review, we summarize the methodology for establishing human podocyte and PTEC cell lines from urine and highlight their importance as kidney disease cell models. We explore the well-established and recent techniques of cell isolation, quantification, immortalization and characterization, and we describe their current and future applications.

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“…Briefly, cells are seeded into a 96 well plate at densities of 0.5 and 1 cell per well. After 14 days, colonies derived from single cells become visible, and can be expanded [78].…”

Section: Molecular and Functional Characterization Of Kidney Derived Cell Linesmentioning

confidence: 99%

Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Bondue

Arcolino

Veys

et al. 2021

Cells

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“…This study also indicated that the induced hepatocyte-like cells displayed altered PCSK9 secretion and LDL uptake, which mimic the pathophysiology of hypercholesterolemia and could be applied in drug screening [ 55 ]. Except for these abovementioned disease modeling techniques with functional experiments, further functional experiments need to be conducted on other UDSC-derived iPSCs after being induced into target cell types [ 41 – 44 , 48 – 50 , 53 ].…”

Section: Udscs Served As Original Cells For Reprogramming Into Dismentioning

confidence: 99%

Urine-Derived Stem Cells: The Present and the Future

Wang

Chen

et al. 2017

Stem Cells International

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Stem cell research provides promising strategies in improving healthcare for human beings. As a noninvasively obtained and easy-to-culture cell resource with relatively low expense, urine-derived stem cells have special advantages. They have been extensively studied on its proliferation ability and differentiation potential and were being reprogrammed to model diseases during the last decade. In this review, we intend to summarize the latest progress on the research of urine-derived stem cells for its broad application mainly in regenerative medicine and disease modeling, as well as in what is challenging currently. This minireview will highlight the potential application of urine-derived stem cells and provides possible direction of further research in the future.

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“…As a proof-of-concept study, we used previously characterized iPSC lines to pursue preliminary mechanistic in vitro studies. [7][8][9] Cardiomyocyte differentiation of iPSCs was achieved by following published protocols 10,11 with details outlined in the eMethods in the Supplement from 1 participant homozygous for the c.699_700dupTA SERPINE1 variant and 1 wildtype control participant. Experimental results examining cardiomyocyte response to angiotensin II and metabolic stress are presented as mean (SEM).…”

Section: Stem Cell-derived Cardiomyocytesmentioning

confidence: 99%

Identification of Cardiac Fibrosis in Young Adults With a Homozygous Frameshift Variant in SERPINE1

et al. 2021

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IMPORTANCECardiac fibrosis is exceedingly rare in young adults. Identification of genetic variants that cause early-onset cardiomyopathy may inform novel biological pathways. Experimental models and a single case report have linked genetic deficiency of plasminogen activator inhibitor-1 (PAI-1), a downstream target of cardiac transforming growth factor β, with cardiac fibrosis. OBJECTIVE To perform detailed cardiovascular phenotyping and genotyping in young adults from an Amish family with a frameshift variant (c.699_700dupTA) in SERPINE1, the gene that codes for PAI-1. DESIGN, SETTING, AND PARTICIPANTSThis observational study included participants from 3 related nuclear families from an Amish community in the primary analysis and participants from the extended family in the secondary analysis.

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Generation of human iPSCs from urine derived cells of a non-affected control subject

Cited by 6 publications

References 4 publications

Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Urine-Derived Stem Cells: The Present and the Future

Identification of Cardiac Fibrosis in Young Adults With a Homozygous Frameshift Variant in SERPINE1

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