Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards “universal-donor” red blood cells

Seifinejad, Ali; Taei, Adeleh; Totonchi, Mehdi; Vazirinasab, Hamed; Hassani, Seyedeh‐Nafiseh; Aghdami, Nasser; Shahbazi, Ebrahim; Yazdi, Reza Salman; Salekdeh, Ghasem Hosseini; Baharvand, Hossein

doi:10.1016/j.bbrc.2009.11.058

Cited by 29 publications

(26 citation statements)

References 35 publications

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“…The cells are similar to embryonic pluripotent stem cells (ESCs) in their morphology, pluripotency marker expression, self-renewal property and ability to differentiate into the three primary germ layers both in vivo and in vitro [2], [3], [4], [5], [6], [7]. However, they do not have the ethical barriers of ESCs [4]. Moreover, a retrospective study by Peyrard et al .…”

Section: Introductionmentioning

confidence: 99%

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

et al. 2014

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Induced pluripotent stem cells (iPSC) are an attractive progenitor source for the generation of in vitro blood products. However, before iPSC-derived erythroid cells can be considered for therapeutic use their similarity to adult erythroid cells must be confirmed. We have analysed the proteome of erythroid cells differentiated from the iPSC fibroblast derived line (C19) and showed they express hallmark RBC proteins, including all those of the ankyrin and 4.1R complex. We next compared the proteome of erythroid cells differentiated from three iPSC lines (C19, OCE1, OPM2) with that of adult and cord blood progenitors. Of the 1989 proteins quantified <3% differed in level by 2-fold or more between the different iPSC-derived erythroid cells. When compared to adult cells, 11% of proteins differed in level by 2-fold or more, falling to 1.9% if a 5-fold threshold was imposed to accommodate slight inter-cell line erythropoietic developmental variation. Notably, the level of >30 hallmark erythroid proteins was consistent between the iPSC lines and adult cells. In addition, a sub-population (10–15%) of iPSC erythroid cells in each of the iPSC lines completed enucleation. Aberrant expression of some cytoskeleton proteins may contribute to the failure of the majority of the cells to enucleate since we detected some alterations in cytoskeletal protein abundance. In conclusion, the proteome of erythroid cells differentiated from iPSC lines is very similar to that of normal adult erythroid cells, but further work to improve the induction of erythroid cells in existing iPSC lines or to generate novel erythroid cell lines is required before iPSC-derived red cells can be considered suitable for transfusion therapy.

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Section: Introductionmentioning

confidence: 99%

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

et al. 2014

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“…While our laboratory has previously shown that the then existing hESC lines are not conducive to culturing universal donor RBCs [6], many additional hESC lines have been generated ever since. Furthermore, the recent advances in hiPSCs have partially obviated this obstacle, as exemplified by the generation of hiPSCs from a Bombay individual whose RBCs lack ABH antigens expression due to the absence of the H gene (FUT1-) and Secretor gene (FUT2-) encoded α 1,2 fucosyltransferase activities [7]. It has been proposed by French researchers that based on their database, as few as 15 hiPSC clones would cover 100% of the needs of all Caucasian patients with rare blood phenotypes/genotypes in France [2].…”

Section: Introductionmentioning

confidence: 99%

Generation and Characterization of Erythroid Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells: An Overview

Chang

Bönig

Papayannopoulou

2011

Stem Cells International

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Because of the imbalance in the supply and demand of red blood cells (RBCs), especially for alloimmunized patients or patients with rare blood phenotypes, extensive research has been done to generate therapeutic quantities of mature RBCs from hematopoietic stem cells of various sources, such as bone marrow, peripheral blood, and cord blood. Since human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be maintained indefinitely in vitro, they represent potentially inexhaustible sources of donor-free RBCs. In contrast to other ex vivo stem-cell-derived cellular therapeutics, tumorigenesis is not a concern, as RBCs can be irradiated without marked adverse effects on in vivo function. Here, we provide a comprehensive review of the recent publications relevant to the generation and characterization of hESC- and iPSC-derived erythroid cells and discuss challenges to be met before the eventual realization of clinical usage of these cells.

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“…ES cells or iPSC can be genetically manipulated using most of the gene transfer techniques developed so far and specific clones can be isolated prior to differentiation processes. In comparison with approaches using donor-derived hematopoietic stem cells, a major advantage of these cell types is the possibility of viable cryopreservation and of unlimited amplification in order to respond to a specific need or to maintain the stock in a bank [82,83], making the stable modification of the genome the most relevant strategy for in vitro production of RBCs from genetically manipulated ES or iPSC.…”

Section: Breaking the Gene In Stem Cellsmentioning

confidence: 99%

Silencing and overexpression of human blood group antigens in transfusion: Paving the way for the next steps

Bagnis

2015

Blood Reviews

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Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards “universal-donor” red blood cells

Cited by 29 publications

References 35 publications

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

Generation and Characterization of Erythroid Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells: An Overview

Silencing and overexpression of human blood group antigens in transfusion: Paving the way for the next steps

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