Generation of excitatory postsynaptic potentials in the hippocampus after functional modification of glycosaminoglycans

Abstract

Experiments on hippocampal slices form 4-week-old rats (n=28) showed that addition of lidase (1.0 and 10.0 U/ml) to the perfusion solution (artificial cerebrospinal fluid) was accompanied by the impaired generation or blockade of excitatory postsynaptic potentials and population spikes in the hippocampal CA1 region during stimulation of Schaffer collaterals. Removal of lidase from this solution normalized the amplitude of evoked responses. Hence, lidase in these concentrations produced a reversible effect on synaptic transmission. Our results indicate that structure and function of glycosaminoglycans in the extracellular matrix determine signal transduction in the nervous tissue.

The crosstalk of hyaluronan-based extracellular matrix and synapses

“…Perfusion of acute hippocampal slices with lidase for 3 min leads to impaired generation or blockade of excitatory postsynaptic potentials and population spikes in the CA1 region (Garkun et al, 2008), which was completely reversible within minutes.…”

The influence of heterocyclic compound-PAMAM dendrimer complexes on evoked electrical responses in slices of hypoxic brain tissue

“…The recovery of the amplitude of EPSP was almost twice as slow under such conditions. Also, during the initial period of hypoxia combined with dendrimer-based complexes, a brief upturn in PS amplitude was always present, reflecting the dissociation of the input-output relationship [11]. The decrease in the ability to form EPSP (input) in the hippocampus under hypoxic conditions in the presence of dendrimer-heterocyclic compound complexes is accompanied by an activation of processes forming PS (output).…”

“…We performed the electrophysiological experiments using 450-µm thick transverse slices of the hippocampus from 4-week old male rats (n = 13) as described earlier [11]. The samples were taken from rats that were also used as a control group in a set of experiments carried out by another group of the During the experiments, the slices were placed in a temperature-controlled BSC-ZT chamber (Harvard Apparatus) at 29ºC and perfused with carbogen-saturated ACSF at a flow rate of 4 ml/min.…”

“…We chose hippocampal slices as the in vitro model to test our hypothesis. The continued presence of intercellular communication has been established both in brain slices and in cell culture [10,11]. Hippocampal cells are involved in the control of memory and learning.…”

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

“…Transverse hippocampal slices 400 µm in thickness were separated from ACSF solution using microtome (Leitz, Germany) with cooling section, which maintains temperature at 3-4°C level [7] . Then the slices were incubated in ex tempore prepared ACSF at 28°C for 30 minutes; ACSF was saturated with carbogen (95% O 2 and 5% CO 2 ) and contained (mmol/L): 124.0 NaCl; 3.0 KCl; 1.25 KH 2 PO 4 ; 1.2 MgCl 2 ; 2.0 CaCl 2 ; 26.0 NaHCO 3 ; 10.0 glucose; pH=7.4 [7] . Slices were put into incubation setter with constant ACSF flow after preincubation (ex tempore).…”