Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-Derived Multipotent Germline Stem Cells

Baba, Shiro; Heike, Toshio; Umeda, Katsutsugu; Iwasa, Takeshi; Kaichi, Shinji; Hiraumi, Yoshimi; Doi, Hiraku; Yoshimoto, Momoko; Kanatsu-Shinohara, Mito; Shinohara, Takashi; Nakahata, Tatsutoshi

doi:10.1634/stemcells.2006-0574

Cited by 46 publications

(32 citation statements)

References 34 publications

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“…Baba et al (2007) utilized an OP9 co-culture system to compare mGS, ES and embryonic germ cells and found a comparable yield in vitro of mesodermal derivatives including endothelial and cardiac cells from mGS, although the derivative cell types were not assessed for function in vivo. 41 Another study found that maGSCs could form functional cardiac cells in vitro but the fate of these cells in vivo is less clear since only a transient fluorescent cell label (CM-DiI) was employed. 42 We similarly found that MASCs spontaneously differentiate into mesodermal derivatives, including contractile cardiac tissue.…”

Section: Therapeutic Implementation Of Mascsmentioning

confidence: 99%

Niche players: Spermatogonial progenitors marked by GPR125

Seandel¹,

Falciatori²,

Shmelkov³

et al. 2008

Cell Cycle

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The undifferentiated spermatogonia of adult mouse testes are composed of both true stem cells and committed progenitors.It is unclear what normally prevents these adult germ cells from manifesting multipotency. The critical elements of the spermatogonial stem cell niche, while poorly understood, are thought to be composed of Sertoli cells with several other somatic cell types in close proximity. We recently discovered a novel orphan G-protein coupled receptor (GPR125) that is restricted to undifferentiated spermatogonia within the testis. GPR125 expression was maintained when the progenitor cells were extracted from the in vivo niche and propagated under growth conditions that recapitulate key elements of the niche. Such conditions preserved the ability of the cells to generate multipotent derivatives, known as multipotent adult spermatogonial derived progenitor cells (MASCs). Upon differentiation, the latter produced a variety tissues including functional endothelium, illustrating the potential applications of such cells. Thus, GPR125 represents a novel target for purifying adult stem and progenitors from tissues, with the goal of developing autologous multipotent cell lines.

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Section: Therapeutic Implementation Of Mascsmentioning

confidence: 99%

Niche players: Spermatogonial progenitors marked by GPR125

Seandel¹,

Falciatori²,

Shmelkov³

et al. 2008

Cell Cycle

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“…Another study, carried out by Baba et al, showed that Flk1 + cells from differentiating GPSCs could give rise to mature cardiomyocytes and endothelial cells as efficiently as ES cells. 57 The same group also demonstrated that transplantation of Flk1 + GPSCs directly into the heart of ischemic mice improved cardiac function. 58 Although 4 weeks after treatment, the number of cardiomyocytes derived from Flk1 + GPSCs were too small to account for the improvement in cardiac function, angiogenesis around ischemic area was enhanced compared to control group, and senescence was also remarkably diminished in the early phase of ischemia.…”

Section: Gpscs and Heart Regenerationmentioning

confidence: 94%

Potential applications of germline cell-derived pluripotent stem cells in organ regeneration

et al. 2011

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“…Embryonic mouse cell tracking and ESC differentiation studies have lead many groups to believe that myocardial and endothelial lineages develop from a common Flk-1-positive progenitor (Kattman et al, 2006). The Flk-1 positive cell population isolated from mESCs differentiates into a higher portion of cardiac muscle than the Flk-1-negative cell population in vivo, however this marker was not specific enough on its own to generate a pure population of precursors cells that exclusively differentiated into cardiomyocytes (Baba et al, 2007a;Baba et al, 2007b;Iida et al, 2005;Nelson et al, 2009). When Flk1+ mESCs were transplanted in vivo into the hearts of a cardiomyopathic mouse, significant improvement in cardiac function was recorded (Adler et al, 2010;Baba et al, 2007b).…”

Section: -Surface Marker Purification Of Es Cellsmentioning

confidence: 99%

Cardiac Tissue Engineering

Momtahan

Castleton

Ford

et al. 2014

Methods in Molecular Biology

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The limited treatment options available for heart disease patients has lead to increased interest in the development of embryonic stem cell (ESC) therapies to replace heart muscle. The challenges of developing usable ESC therapeutic strategies are associated with the limited ability to obtain a pure, defined population of differentiated cardiomyocytes, and the design of in vivo cell delivery platforms to minimize cardiomyocyte loss. These challenges were addressed in Chapter 2 by designing a cardiomyocyte selectable progenitor cell line that permitted evaluation of a collagen- iii and function. Notably, no significant differences in cell survival, cardiac phenotype, and cardiomyocyte function were detected with the addition of the RGD domain to the collagen scaffold. Thus, in summary, these three studies have resulted in the identification of a potential cell surface marker for ESC-derived cardiomyocyte purification, and prove that collagen-based scaffolds can sustain ES-cardiomyocyte growth and function. This has set the framework for further studies that will move the field closer to obtaining a safe and effective delivery strategy for transplanting ESCs onto human hearts.

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Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-Derived Multipotent Germline Stem Cells

Abstract: ABSTRACT

Cited by 46 publications

References 34 publications

Niche players: Spermatogonial progenitors marked by GPR125

Niche players: Spermatogonial progenitors marked by GPR125

Potential applications of germline cell-derived pluripotent stem cells in organ regeneration

Cardiac Tissue Engineering

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