Potential applications of germline cell-derived pluripotent stem cells in organ regeneration

Fagoonee, Sharmila; Pellicano, Rinaldo; Silengo, Lorenzo; Altruda, Fiorella

doi:10.4161/org.7.2.16284

Cited by 12 publications

(7 citation statements)

References 71 publications

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“…In the last decade, pluripotent stem cells derived from spermatogonial stem cells (SSCs) have been much studied as a potential alternative to these above-mentioned cells[ 9 ]. Mouse SSCs can be isolated and cultured on mouse embryonic fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Long Term Liver Engraftment of Functional Hepatocytes Obtained from Germline Cell-Derived Pluripotent Stem Cells

et al. 2015

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One of the major hurdles in liver gene and cell therapy is availability of ex vivo-expanded hepatocytes. Pluripotent stem cells are an attractive alternative. Here, we show that hepatocyte precursors can be isolated from male germline cell-derived pluripotent stem cells (GPSCs) using the hepatoblast marker, Liv2, and induced to differentiate into hepatocytes in vitro. These cells expressed hepatic-specific genes and were functional as demonstrated by their ability to secrete albumin and produce urea. When transplanted in the liver parenchyma of partially hepatectomised mice, Liv2-sorted cells showed regional and heterogeneous engraftment in the injected lobe. Moreover, approximately 50% of Y chromosome-positive, GPSC-derived cells were found in the female livers, in the region of engraftment, even one month after cell injection. This is the first study showing that Liv2-sorted GPSCs-derived hepatocytes can undergo long lasting engraftment in the mouse liver. Thus, GPSCs might offer promise for regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Long Term Liver Engraftment of Functional Hepatocytes Obtained from Germline Cell-Derived Pluripotent Stem Cells

et al. 2015

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“…SSCs are derived from adult testes, and have the propensity to convert to pluripotent stem cells sharing features with ESCs in vitro. We and others have demonstrated that mouse SSCs can be efficiently induced to differentiate into functional HLCs in vitro, and that the transplanted HLCs engraft into mice livers [71][72][73][74][75]. The pluripotency characteristics of human SSCs are still being investigated.…”

Section: Liver Stem Cellsmentioning

confidence: 99%

Evolving Cell-Based and Cell-Free Clinical Strategies for Treating Severe Human Liver Diseases

Cernigliaro

Peluso

Zedda

et al. 2020

Cells

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Liver diseases represent a major global health issue, and currently, liver transplantation is the only viable alternative to reduce mortality rates in patients with end-stage liver diseases. However, scarcity of donor organs and risk of recidivism requiring a re-transplantation remain major obstacles. Hence, much hope has turned towards cell-based therapy. Hepatocyte-like cells obtained from embryonic stem cells or adult stem cells bearing multipotent or pluripotent characteristics, as well as cell-based systems, such as organoids, bio-artificial liver devices, bioscaffolds and organ printing are indeed promising. New approaches based on extracellular vesicles are also being investigated as cell substitutes. Extracellular vesicles, through the transfer of bioactive molecules, can modulate liver regeneration and restore hepatic function. This review provides an update on the current state-of-art cell-based and cell-free strategies as alternatives to liver transplantation for patients with end-stage liver diseases.

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“…Despite all hurdles and controversies, clinical trials in treatment of spinal cord injury, macular degeneration of retina, type 1 diabetes and heart failure are under way [57]. [58]. The first clue that GPSCs can be directed to differentiate into hepatocyte-like cells came from the study of Guan et al [59].…”

Section: Adult Pluripotent Stem Cellsmentioning

confidence: 99%

Prospects for Adult Stem Cells in the Treatment of Liver Diseases

Fagoonee

Famulari

Silengo

et al. 2016

Stem Cells and Development

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Hepatocytes constitute the main bulk of the liver and perform several essential functions. After injury, the hepatocytes have a remarkable capacity to regenerate and restore functionality. However, in some cases, the endogenous hepatocytes cannot replicate or restore the function, and liver transplantation, which is not exempt of complications, is required. Stem cells offer in theory the possibility of generating unlimited supply of hepatocytes in vitro due to their capacity to self-renew and differentiate when given the right cues. Stem cells isolated from an array of tissues have been investigated for their capacity to differentiate into hepatocyte-like cells in vitro and are employed in rescue experiments in vivo. Adult stem cells have gained in attractiveness over embryonic stem cells for liver cell therapy due to their origin, multipotentiality, and the possibility of autologous transplantation. This review deals with the promise and limitations of adult stem cells in clinically restoring liver functionality.

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Potential applications of germline cell-derived pluripotent stem cells in organ regeneration

Cited by 12 publications

References 71 publications

Long Term Liver Engraftment of Functional Hepatocytes Obtained from Germline Cell-Derived Pluripotent Stem Cells

Long Term Liver Engraftment of Functional Hepatocytes Obtained from Germline Cell-Derived Pluripotent Stem Cells

Evolving Cell-Based and Cell-Free Clinical Strategies for Treating Severe Human Liver Diseases

Prospects for Adult Stem Cells in the Treatment of Liver Diseases

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